Detection of hepatitis C virus RNA in paraffin‐embedded tissues from patients with non‐Hodgkin's lymphoma

Paydaş, Semra; Ergïn, Melek; Tanrıverdi, Kahraman; Yavuz, Sinan; Dişel, Umut; Kilic, Nil Banu; Erdoğan, Şeyda; Şahin, Berksoy; Tuncer, İlhan; Burgut, Refik

doi:10.1002/ajh.20092

Cited by 23 publications

(18 citation statements)

References 47 publications

(38 reference statements)

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“…They found that 51.7% of the 29 cases of splenic DLB were HCV-Ab-positive, and found the HCV RNA genome in lymphoma tissue in six of the seven cases examined, and the expression of NS3 protein and E2 protein in cell cytoplasm in five of the seven cases (as in our case) [15]. Moreover, Paydas et al reported that the HCV RNA genome was detected in 37% of 30 cases of NHL (including 15 cases of DLB) and that the serum level of HCV RNA was high in 23.3% of the cases [16]. There have also been several other studies on the expression of HCV NS3 protein and E2 protein, and the detection of the HCV RNA genome in lymphoma tissues [8,17,18], and the results of those studies also suggest a strong association between NHL and HCV infection.…”

Section: Discussionsupporting

confidence: 45%

HCV non-structural protein 3 and HCV RNA genome in non-Hodgkin lymphoma and transition of the serum HCV RNA level: a retrospective analysis in one institution

et al. 2008

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There have been various reports on the association of hepatitis C virus (HCV) infection with B lymphocyte proliferative disorders, such as non-Hodgkin lymphoma (NHL). We experienced a case (Case 1) of anti-HCV antibody (HCV-Ab)-positive NHL in which HCV nonstructural protein 3 (NS3) expression was observed in lymphoma tissue at the time of recurrence and in which the serum HCV RNA level exhibited a transient increase prior to recurrence. We investigated the HCV RNA genome in lymphoma tissue in Case 1, and it could be detected at recurrence. We also investigated HCV NS3 protein expression in lymphoma tissue and changes in serum HCV RNA level during the clinical course in four other cases of HCV-Ab-positive NHL treated in our hospital. We examined lymphoma tissues for HCV NS3 protein expression in four of the five cases, but it was not identified except for in Case 1 at recurrence. In three cases with no recurrence, serum HCV RNA levels showed a tendency to decrease after completion of chemotherapy and became stable thereafter. Further studies are necessary to clarify the association between serum HCV RNA and the onset and exacerbation of NHL.

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Section: Discussionsupporting

confidence: 45%

HCV non-structural protein 3 and HCV RNA genome in non-Hodgkin lymphoma and transition of the serum HCV RNA level: a retrospective analysis in one institution

et al. 2008

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“…Various viral and environmental factors are held responsible for the development of NHL (Barista et al, 1994;Paydas et al, 2004). Spinelli et al investigated the relationship between the serum organochlorine levels and NHL.…”

Section: Discussionmentioning

confidence: 99%

Non-Hodgkin Lymphoma and Pesticide Exposure in Turkey

Yıldırım¹,

Karakilinc²,

Yıldız³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Background: Non-Hodgkin lymphoma (NHL) is a solid tumour of lymphocytes, important elements in the immune system. According to 2006 data, in Turkey the incidence was 6.5 per 100,000 in males, and 4.4 in females. The relationship between the use of pesticides and development of NHL has been extensively investigated in many studies, and it has been demonstrated that the risk of NHL is increased by exposure to such compounds. Antalya is a region of intensive agricultural activity. In this study, the relationship between the incidence of lymphoma in Antalya and the amount of pesticides employed was investigated. Materials and Methods: The study used data from 1995 to 2010 on the patients from the databank of TR Ministry of Health, Antalya Provincial Health Directorate, Cancer Registration Center and the patients who were histopathologically diagnosed with NHL during these years. Results: The relationship between the amount of pesticide used and the incidence was studied with the Spearman correlation analysis and the p value was found as 0.05. The correlation coefficient was 0.497. An increase in the NHL incidence over the years was identified, with a 2.42-fold increment found from 1995 to 2005 and a 2.77 fold elevation from 1995 to 2010. The use of pesticides increased 1.89 fold over the same period. Conclusions: Our study investigated the relationship of the pesticides used with NHL patients diagnosed during the same year. Since the time elapsing after exposure to pesticides until the development of cancer is not clear, no comparison can be made at present. We believe that the increase in use of pesticides since 1995 may be associated with the increase in the incidence of NHLand therefore that further studies on the issue including measurements of serum pesticide levels, are required.,

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“…The infection of HCV results in a higher rate of chronic infection compared to HBV infection with approximately 80% of infected subjects showing viral persistence and chronic liver diseases [5,6], thus an accurate diagnosis is essential to identify the presence of HCV RNA in apparently symptom free carriers [7,8]. Patients with HCV infection develop chronic liver disease, in this group, about 30% progress to cirrhosis, and 1-2% develops hepatocellular carcinoma per year [9,10].…”

Section: Introductionmentioning

confidence: 99%

Quantitation assay of hepatitis C virus RNA using real-time PCR technique

Taha¹,

Ali²,

Sabeer³

2017

Cihan Univ.-Erbil Sci. J.

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Hepatitis C virus is a major cause of acute and chronic infections leading to fibrosis, cirrhosis, and hepatocellular carcinoma. A real-time PCR analysis has been employed successfully for both basic research and clinical applications. The ability to monitor the real-time progress of the amplification greatly improves the PCR-based quantitation of Hepatitis C virus RNA. The study was determine the viral load of hepatitis C virus RNA by real-time PCR technique and the influence of the gender on HCV viral load. The presence of HCV antibodies were assessed in clinically suspected HCV infection patients using commercial HCV Ab ELISA test kit, then all positive test were tested for detection and quantitative of HCV RNA by real-time PCR at Public Health Laboratory in Erbil, Iraq. Among 445 patients of HCV sero positive with ELISA screening test, RNA HCV was detected in 195(43.82%) patients by quantitative realtime PCR. Based on the distribution of viral load, 38.97% of patients had viral loads of 10 6 IU/ml, 0.51% had viral loads between 10 8 and 10 9 IU/mL, and 6.67% had 10 3 IU/ml.In conclusion, the highest rate of HCV was detected among sero positive males.

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Detection of hepatitis C virus RNA in paraffin‐embedded tissues from patients with non‐Hodgkin's lymphoma

Cited by 23 publications

References 47 publications

HCV non-structural protein 3 and HCV RNA genome in non-Hodgkin lymphoma and transition of the serum HCV RNA level: a retrospective analysis in one institution

HCV non-structural protein 3 and HCV RNA genome in non-Hodgkin lymphoma and transition of the serum HCV RNA level: a retrospective analysis in one institution

Non-Hodgkin Lymphoma and Pesticide Exposure in Turkey

Quantitation assay of hepatitis C virus RNA using real-time PCR technique

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