An arabinogalactan from Lycium barbarum attenuates DSS-induced chronic colitis in C57BL/6J mice associated with the modulation of intestinal barrier function and gut microbiota

Abstract

Ulcerative colitis (UC) is an incurable chronic inflammation of the enteric tract. The aim of this study was to investigate the protective effects of arabinogalactan from Lycium barbarum on DSS-induced...

Impact ofLycium barbarumarabinogalactan on the fecal metabolome in a DSS-induced chronic colitis mouse model

“…28,29 Our previous studies indicated that LBP-3 relieved the symptoms (e.g., diarrhea and weight loss) of DSSinduced colitis in mice, which was associated with the composition and diversity of gut microbiota. 17 However, little is known about how the gut microbiota affects the metabolic changes in chronic colitis mice after supplementation with arabinogalactan from L. barbarum. Herein, besides considering specific organisms of the microbiome, untargeted and targeted LC-MS metabolomics in feces were performed to investigate the metabolome and metabolic pathways altered by LBP-3, elucidating the possible mechanism of LBP-3 against UC.…”

“…LBP-3 ( M w 100 ± 4 kDa) was prepared by our previously reported method. 17 Briefly, the crude polysaccharides were extracted with hot water (80 °C, 2 h) and ethanol precipitation (four times volume of ethanol) from L. barbarum fruits (Ningqi no. 1, Ningxia, China).…”

“…The above SCFAs were positively correlated with Ruminococcaceae, Butyricimonas, Alloprevotella, Alistipes, Paraprevotella and Candidatus_Saccharimonas in our previous study. 17 Except for amino acid metabolism and organic acid metabolism, it has also been found that purine and pyrimidine metabolism was reduced in UC mice and patients 32,56 and supplementation with LBP-3 also changed the levels of purines, pyrimidines and their derivatives.…”

“…edly reduced the relative abundance of Parasutterella (belonging to the phylum of Proteobacteria) and consequently lowered the levels of cysteine, taurine, and inflammatory cytokines (e.g., TNF-α and IL-1β), which was consistent with previous studies. 17,[63][64][65] In addition, Odoribacter can generate butyrate and has a highly active pentose phosphate pathway as well as the capability of tryptophan metabolism in the intestinal environment. 66,67 As expected, LBP-3 and ASA treatment significantly enhanced the abundance of Odoribacter and the contents of butyrate and tryptophan.…”

“…15,16 Our previous study has demonstrated that supplementation with Lycium barbarum arabinogalactan (LBP-3) with a characteristic structure could modulate the composition of gut microbiota and improve the gut barrier function, thus alleviating DSS-induced colitis in mice. 17 Based on the metabolic disorders in UC and the modulation of LBP-3 of gut microbiota, we hypothesized that LBP-3 could possibly recover the metabolic profiles of gut microbiota in DSS-induced colitis. Therefore, a combination of 16S rRNA gene sequencing and HPLC-MS-based metabolomics in feces was performed to investigate the influence of gut microbiota on the metabolic profiles after LBP-3 treatment in chronic colitis in the present study.…”