An Aqueous High-Performance Liquid Chromatographic Procedure for the Determination of 5-Hydroxymethylfurfural in Honey and Other Sugar-containing Materials

Abstract

ABSTRA ABSTRA ABSTRA ABSTRA ABSTRACT CT CT CT CT: A new : A new : A new : A new : A new, all aqueous high-per , all aqueous high-per , all aqueous high-per , all aqueous high-per , all aqueous high-perfor for for for formance liquid chr mance liquid chr mance liquid chr mance liquid chr mance liquid chromatogr omatogr omatogr omatogr omatography (HPL aphy (HPL aphy (HPL aphy (HPL aphy (HPLC) pr C) pr C) pr C) pr C) procedur ocedur ocedur ocedur ocedure for the deter e for the deter e for the deter e for the deter e for the determinamina-mina-minamination of 5-hydroxymethylfurfural (HMF) in honey and other sugar-containing materials was developed without tion of 5-hydroxymethylfurfural (HMF) in honey and other sugar-containing materials was developed without tion of 5-hydroxymethylfurfural (HMF) in honey and other sugar-containing materials was developed without tion of 5-hydroxymethylfurfural (HMF) in honey and other sugar-containing materials was developed without tion of 5-hydroxymethylfurfural (HMF) in honey and other sugar-containing materials was developed without harmful chemicals. The method involved dilution of the sample in water and the analysis of the diluent using an harmful chemicals. The method involved dilution of the sample in water and the analysis of the diluent using an harmful chemicals. The method involved dilution of the sample in water and the analysis of the diluent using an harmful chemicals. The method involved dilution of the sample in water and the analysis of the diluent using an harmful chemicals. The method involved dilution of the sample in water and the analysis of the diluent using an HPLC system equipped with an absorbance detector and a 30-mm sulfonated poly(styrene-divinylbenzene) HPLC system equipped with an absorbance detector and a 30-mm sulfonated poly(styrene-divinylbenzene) HPLC system equipped with an absorbance detector and a 30-mm sulfonated poly(styrene-divinylbenzene) HPLC system equipped with an absorbance detector and a 30-mm sulfonated poly(styrene-divinylbenzene) HPLC system equipped with an absorbance detector and a 30-mm sulfonated poly(styrene-divinylbenzene) cation ex cation ex cation ex cation ex cation exchange column. change column. change column. change column. change column. W W W W Water was used as the mobile phase at a high flo ater was used as the mobile phase at a high flo ater was used as the mobile phase at a high flo ater was used as the mobile phase at a high flo ater was used as the mobile phase at a high flow r w r w r w r w rate of 2.00 mL/min ate of 2.00 mL/min ate of 2.00 mL/min ate of 2.00 mL/min ate of 2.00 mL/min . . .. . U U U U Using this high sing this high sing this high sing this high sing this high flow rate in conjunction with a column heater set at 55 °C, HMF eluted in less than 4 min. Overall precision was flow rate in conjunction with a column heater set at 55 °C, HMF eluted in less than 4 min. Overall precision was flow rate in conjunction with a column heater set at 55 °C, HMF eluted in less than 4 min. Overall precision w...

In house validation from direct determination of 5-hydroxymethyl-2-furfural (HMF) in Brazilian corn and cane syrups samples by HPLC–UV

“…Highest levels of HMF were observed in corn syrup samples (406.6 -2121.3). Besides this, for most samples analyzed the concentrations observed for HMF were also much higher than those reported in the literature by other researchers (1.3 -1410 mg kg-1) (Vorlová, Borkovcová, Kalábová, & Večerek, 2006;Risner, Kiser, & Dube, 2006). Cane syrups were characterized by lower amounts of HMF (109.2 -893.1 mg kg -1 ) ( Table 3) and so as observed above for corn syrup, the concentrations of this contaminant found in Brazilian samples were higher than that reported in literature (100 -300 mg kg -1 ) (Kowalski, Lukasiewicz, Duda-Chodak, & Zieć, 2013).…”

5-Hydroxymethylfurfural (HMF) levels in honey and other food products: effects on bees and human health

“…The presence of HMF in brown sugar may be due to the addition of treacle to the preparation preserved at 50 °C to maintain its liquidity. Similarly, using HPLC, Risner et al [ 139 ] found similar ranges of HMF concentration (11.9–16.4 and 12.3–23.3 mg/kg) for light and dark-brown sugars, respectively.…”

Effect of Storage on 5-Hydroxymethylfurfural (HMF) Formation and Color Change in Jams

“…[6,7,15,16] However, studies related with color changes together with HMF formation during storage in jams are limited. This study aims at investigating the relationship of color change with HMF formation in strawberry, cherry and apricot jams at different storage temperatures and times.…”