An aptasensor for the label-free detection of thrombin based on turn-on fluorescent DNA-templated Cu/Ag nanoclusters

Zhang, Baozhu; Wei, Chunying

doi:10.1039/d0ra04609d

Cited by 13 publications

(4 citation statements)

References 57 publications

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“…In addition to fluorescence, gaining a more comprehensive understanding of MNCs can be achieved through the use of both transmission electron microscope (TEM), high-resolution transmission electron microscope (HRTEM), Fourier transform infrared spectroscopy (FTIR), and nuclear magnetic resonance (NMR). − TEM and HRTEM can be used to determine the morphology and size of metal nanoclusters and display their three-dimensional structural information. FTIR and NMR can be used to determine the surface chemical properties of metal nanoclusters, such as surface chemical modification or molecular-based interaction . Therefore, the characterization of metal nanoclusters requires comprehensive application of many technologies to obtain information on their structure, composition, surface properties, and optical properties and provide basic support for their application and performance regulation.…”

Section: Properties Of Dna-templated Metal Nanoclustersmentioning

confidence: 99%

“…FTIR and NMR can be used to determine the surface chemical properties of metal nanoclusters, such as surface chemical modification or molecular-based interaction. 53 Therefore, the characterization of metal nanoclusters requires comprehensive application of many technologies to obtain information on their structure, composition, surface properties, and optical properties and provide basic support for their application and performance regulation.…”

Section: Properties Of Dna-templated Metal Nanoclustersmentioning

confidence: 99%

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Recent Advances of DNA-Templated Metal Nanoclusters for Food Safety Detection: From Synthesis, Applications, Challenges, and Beyond

Tang,

Lu,

Wang

et al. 2024

J. Agric. Food Chem.

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Food safety concerns have become a significant threat to human health and well-being, catching global attention in recent years. As a result, it is imperative to research conceptually novel biosensing and effective techniques for food matrices detection. Currently, DNA-templated metal nanoclusters (DNA-MNCs) are considered as one of the most promising nanomaterials due to their excellent properties in biosensing. While DNA-MNCs have garnered increasing interest, the reviews of design strategies, applications, and futuristic prospects for biosensing have been hardly found especially in food safety. The synthesis of DNA-MNCs and their use as biosensing materials in food contamination detection, including pathogenic bacteria, toxins, heavy metals, residues of pesticides, and others were comprehensively reviewed. In addition, we summarize the properties of DNA-MNCs briefly and discuss the challenges and future trends. The application of DNA-MNCs powered biosensing has been demonstrated and actively studied, which is a promising paradigm for food safety testing that can supplement or even replace current existing methods. Despite the challenges of difficulty regulating accurately, poor stability, low quantum yield, and difficult commercial transformation, the application prospects of DNA-MNCs biosensors are promising. This review aims to provide insights and directions for the future development of DNA-MNCs based food detection technology.

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Section: Properties Of Dna-templated Metal Nanoclustersmentioning

confidence: 99%

Section: Properties Of Dna-templated Metal Nanoclustersmentioning

confidence: 99%

Recent Advances of DNA-Templated Metal Nanoclusters for Food Safety Detection: From Synthesis, Applications, Challenges, and Beyond

Tang,

Lu,

Wang

et al. 2024

J. Agric. Food Chem.

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“…A simple aptasensor based on DNA-Cu/Ag NCs was developed for TB detection, wherein the DNA patterns were made up of a Cu/Ag NC-nucleation section at either end and a TBA section in the center. Due to the structural change of the aptamer in the attendance of TB, two DNA-Cu/Ag NCs approach close to each other, causing an increase in the fluorescence intensity of DNA-Cu/Ag NCs [ 107 ].…”

Section: Optical Biosensorsmentioning

confidence: 99%

Recent Progresses in Development of Biosensors for Thrombin Detection

et al. 2022

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Thrombin is a serine protease with an essential role in homeostasis and blood coagulation. During vascular injuries, thrombin is generated from prothrombin, a plasma protein, to polymerize fibrinogen molecules into fibrin filaments. Moreover, thrombin is a potent stimulant for platelet activation, which causes blood clots to prevent bleeding. The rapid and sensitive detection of thrombin is important in biological analysis and clinical diagnosis. Hence, various biosensors for thrombin measurement have been developed. Biosensors are devices that produce a quantifiable signal from biological interactions in proportion to the concentration of a target analyte. An aptasensor is a biosensor in which a DNA or RNA aptamer has been used as a biological recognition element and can identify target molecules with a high degree of sensitivity and affinity. Designed biosensors could provide effective methods for the highly selective and specific detection of thrombin. This review has attempted to provide an update of the various biosensors proposed in the literature, which have been designed for thrombin detection. According to their various transducers, the constructions and compositions, the performance, benefits, and restrictions of each are summarized and compared.

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“…Among these nanoclusters, DNA-templated AgNCs (DNA/AgNCs) have attracted special interest as a novel type of fluorophores. Compared with organic dyes and quantum dots (QDs), DNA/AgNCs have the superiority such as longer fluorescence lifetime, higher quantum yield and easy synthesis, low cost, and low toxicity [13,14]. In addition, the formation of AgNCs on the nucleation domain has no significant influence on the other parts of a DNA molecule and the remaining DNA domains still can fold and hybridize [15].…”

Section: Introductionmentioning

confidence: 99%

A novel DNA detection using spherical identification probe and strand displacement reaction-initiated silver nanocluster switch

et al. 2023

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Herein, we developed a novel fluorescent assay using spherical identification probes and toehold-mediated strand displacement reaction-initiated silver nanoclusters (AgNCs) "on-off" signal switch. In this strategy, the target was captured by the spherical probes to induce the activity of exonuclease III (Exo III), catalyzing the cyclic cleavage of substrates to produce a mass of trigger strands. After magnetic bead separation, the intermediates in the supernatant activated downstream toeholdmediated strand displacement reaction to change the structure of silver nanocluster templates, leading to fluorescence intensity reduction. Furthermore, it is demonstrated that the application of spherical identification probes could reduce the signal leakage and the limit of detection. In addition, AgNCs with perfect optical property were ingeniously combined to realize signal output, which reduced the cost and time of synthesis. Under the optimal conditions, the sensing method displayed a good linear range from 250 pM to 25 nM with a detectable minimum concentration of 250 pM. And the practical application potential in complex biological matrices was also evaluated. Considering these advantages, this constructed strategy opens a new path for nucleic acid detection with better performance.

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An aptasensor for the label-free detection of thrombin based on turn-on fluorescent DNA-templated Cu/Ag nanoclusters

Abstract: A highly sensitive thrombin aptasensor was constructed based on the alteration of the aptamer conformation induced by the target recognition and the turn-on fluorescence due to the proximity of two darkish DNA-templated copper/silver nanoclusters (DNA-Cu/Ag NCs).

Cited by 13 publications

References 57 publications

Recent Advances of DNA-Templated Metal Nanoclusters for Food Safety Detection: From Synthesis, Applications, Challenges, and Beyond

Recent Advances of DNA-Templated Metal Nanoclusters for Food Safety Detection: From Synthesis, Applications, Challenges, and Beyond

Recent Progresses in Development of Biosensors for Thrombin Detection

A novel DNA detection using spherical identification probe and strand displacement reaction-initiated silver nanocluster switch

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