An aptamer-tethered DNA origami amplifier for sensitive and accurate imaging of intracellular microRNA

Xing, Chao; Chen, Shan; Lin, Qitian; Lin, Yuhong; Wang, Min; Wang, Jun; Lü, Chunhua

doi:10.1039/d1nr06399e

Cited by 14 publications

(6 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[74] Complementing structural methods, fluorescence-based techniques have gained prominence. [75,76] Dai et al integrated DNA origami-localized catalytic hairpin assembly reactions for intracellular miR-21 detection via fluorescence imaging (Figure 3D). They engineered DNA origami with an array of hairpins, restricting the reaction and enhancing the spatial resolution.…”

Section: Functionalized Dna Origami For Nucleic Acid Detectionmentioning

confidence: 99%

Functionalized DNA Origami‐Enabled Detection of Biomarkers

Yuan,

Zhou,

et al. 2024

ChemBioChem

View full text Add to dashboard Cite

Biomarkers are crucial physiological and pathological indicators in the host. Over the years, numerous detection methods have been developed for biomarkers, given their significant potential in various biological and biomedical applications. Among these, the detection system based on functionalized DNA origami has emerged as a promising approach due to its precise control over sensing modules, enabling sensitive, specific, and programmable biomarker detection. We summarize the advancements in biomarker detection using functionalized DNA origami, focusing on strategies for DNA origami functionalization, mechanisms of biomarker recognition, and applications in disease diagnosis and monitoring. These applications are organized into sections based on the type of biomarkers—nucleic acids, proteins, small molecules, and ions—and concludes with a discussion on the advantages and challenges associated with using functionalized DNA origami systems for biomarker detection.

show abstract

Section: Functionalized Dna Origami For Nucleic Acid Detectionmentioning

confidence: 99%

Functionalized DNA Origami‐Enabled Detection of Biomarkers

Yuan,

Zhou,

et al. 2024

ChemBioChem

View full text Add to dashboard Cite

show abstract

“…Their addressability endows them with the unique advantage of presenting biofunctional components with nanometric precision [6]. Furthermore, DNA origami's low toxicity has allowed their use for the targeted transport of small molecular drugs, proteins, aptamers [7], nanoparticles, antibodies [8], or quantum dots [9] to cells. Specifically, doxorubicin, which can intercalate into grooves of DNA [10], has been widely used for drug delivery experiments with DNA origami nanostructures [11,12].…”

Section: Introductionmentioning

confidence: 99%

Wireframe DNA Origami for the Cellular Delivery of Platinum(II)-Based Drugs

De Luca,

Wang,

Baars

et al. 2023

IJMS

View full text Add to dashboard Cite

The DNA origami method has revolutionized the field of DNA nanotechnology since its introduction. These nanostructures, with their customizable shape and size, addressability, nontoxicity, and capacity to carry bioactive molecules, are promising vehicles for therapeutic delivery. Different approaches have been developed for manipulating and folding DNA origami, resulting in compact lattice-based and wireframe designs. Platinum-based complexes, such as cisplatin and phenanthriplatin, have gained attention for their potential in cancer and antiviral treatments. Phenanthriplatin, in particular, has shown significant antitumor properties by binding to DNA at a single site and inhibiting transcription. The present work aims to study wireframe DNA origami nanostructures as possible carriers for platinum compounds in cancer therapy, employing both cisplatin and phenanthriplatin as model compounds. This research explores the assembly, platinum loading capacity, stability, and modulation of cytotoxicity in cancer cell lines. The findings indicate that nanomolar quantities of the ball-like origami nanostructure, obtained in the presence of phenanthriplatin and therefore loaded with that specific drug, reduced cell viability in MCF-7 (cisplatin-resistant breast adenocarcinoma cell line) to 33%, while being ineffective on the other tested cancer cell lines. The overall results provide valuable insights into using wireframe DNA origami as a highly stable possible carrier of Pt species for very long time-release purposes.

show abstract

“…Consequently, DNA nanomaterials modified by DNA assembly have improved specificity and selectivity, allowing for the more precise control of a nanomaterial structure and further speciation of nanoprobes for specific diseases. Artificial DNA nanomaterials, such as oligonucleotide-modified nanomaterials, DNA origami and three-dimensional nanomaterials, show promising potential in the field of nanomedicine [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

The Amplified DNA Logic Gates Based on Aptamer–Receptor Recognition for Cell Detection and Bioimaging

Wang,

Wu,

Cao

et al. 2023

Biosensors

View full text Add to dashboard Cite

A powerful and accurate method for identifying and isolating cells would be of great importance due to its sensitivity, gentleness and effectiveness. Here, we designed a receptor-based DNA logic device that allows Boolean logic analysis of multiple cells. For ease of expression, the molecules on the cell surface that can bind to the aptamer are referred to as “receptors”. This DNA logic device sends signals based on cell surface sgc8c and sgc4f receptor expression by performing NOT, NOR, AND and OR logic operations, and amplifies and evaluates the signals using HCR. Meanwhile, the release of ICG from the endopore of HMSNs is controlled by affecting structural changes in the DNA logic device. This approach can accurately identify and treat multiple cells on demand based on the presence or absence of cell-specific receptors, facilitating the development of personalized medicine.

show abstract

An aptamer-tethered DNA origami amplifier for sensitive and accurate imaging of intracellular microRNA

Abstract: Accurate detection and imaging of low-abundant microRNAs (miRNAs) in living cells are essential for diagnosis and prognosis of diseases. Designing nanoprobes with resistance to enzyme degradation and effective cell-binding as...

Cited by 14 publications

References 41 publications

Functionalized DNA Origami‐Enabled Detection of Biomarkers

Functionalized DNA Origami‐Enabled Detection of Biomarkers

Wireframe DNA Origami for the Cellular Delivery of Platinum(II)-Based Drugs

The Amplified DNA Logic Gates Based on Aptamer–Receptor Recognition for Cell Detection and Bioimaging

Contact Info

Product

Resources

About