The Amplified DNA Logic Gates Based on Aptamer–Receptor Recognition for Cell Detection and Bioimaging

Abstract

A powerful and accurate method for identifying and isolating cells would be of great importance due to its sensitivity, gentleness and effectiveness. Here, we designed a receptor-based DNA logic device that allows Boolean logic analysis of multiple cells. For ease of expression, the molecules on the cell surface that can bind to the aptamer are referred to as “receptors”. This DNA logic device sends signals based on cell surface sgc8c and sgc4f receptor expression by performing NOT, NOR, AND and OR logic operations, and amplifies and evaluates the signals using HCR. Meanwhile, the release of ICG from the endopore of HMSNs is controlled by affecting structural changes in the DNA logic device. This approach can accurately identify and treat multiple cells on demand based on the presence or absence of cell-specific receptors, facilitating the development of personalized medicine.

Fluorogenic Aptamer-Based Hybridization Chain Reaction for Signal-Amplified Imaging of Apurinic/Apyrimidinic Endonuclease 1 in Living Cells

“…Moreover, the creation of a binary aptamer further broadens the applications of FAs. Many entropy-driven catalytic hairpin assembly systems (CHAs) have utilized FAs as reporters to achieve sensitive molecule detection [ 7 , 12 , 14 , 15 ]. Compared with the enzyme-mediated signal amplification system that is usually used to achieve highly sensitive detection in vitro, a CHA has a moderate amplification efficiency.…”

“…A hybridization chain reaction (HCR) is an entropy-driven self-assembly system that can be used to amplify the detection signal [ 13 , 14 , 15 , 16 ]. Benefiting from high efficiency and simplicity, HCRs have been broadly applied for imaging molecules in living cells [ 17 , 18 ].…”