An aptamer-based electrochemical biosensor for the detection of Salmonella

Ma, Xiaoyuan; Jiang, Yihui; Jia, Fei; Yu, Ye; Chen, Jie; Wang, Zhouping

doi:10.1016/j.mimet.2014.01.003

Cited by 196 publications

(73 citation statements)

References 26 publications

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“…LOD is 10 CFU/g in biological samples.Book et al (2011), Joshi et al (2009); Jyoti et al (2011), and Singh et al (2012) Electrochemical aptasensor. LOD is 3 CFU/ml in bacterial suspension.Ma et al (2014) S. typhimurium whole live cellsDNASandwich-type system with fluorescent detection. LOD is 25 CFU/ml in bacterial suspension.Duan et al (2013a) S. typhimurium whole live cellsDNAImpedimetric aptasensor.…”

Section: Antiviral Aptamersmentioning

confidence: 99%

“…Therefore, both aptamers were considered to be suitable for the development of detection systems. Aptamer 33 was successfully applied for the concentration and detection of pathogenic bacteria in samples obtained from natural reservoirs (such as Ganges and Gomati rivers) (Jyoti et al, 2011; Singh et al, 2012) and also for the development of an electrochemical biosensor (Ma et al, 2014). …”

Section: Antibacterial Aptamersmentioning

confidence: 99%

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Aptamers against pathogenic microorganisms

Davydova

Vorobjeva

Pyshnyi

et al. 2015

Critical Reviews in Microbiology

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An important current issue of modern molecular medicine and biotechnology is the search for new approaches to early diagnostic assays and adequate therapy of infectious diseases. One of the promising solutions to this problem might be a development of nucleic acid aptamers capable of interacting specifically with bacteria, protozoa, and viruses. Such aptamers can be used for the specific recognition of infectious agents as well as for blocking of their functions. The present review summarizes various modern SELEX techniques used in this field, and of several currently identified aptamers against viral particles and unicellular organisms, and their applications. The prospects of applying nucleic acid aptamers for the development of novel detection systems and antibacterial and antiviral drugs are discussed.

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Section: Antiviral Aptamersmentioning

confidence: 99%

Section: Antibacterial Aptamersmentioning

confidence: 99%

Aptamers against pathogenic microorganisms

Davydova

Vorobjeva

Pyshnyi

et al. 2015

Critical Reviews in Microbiology

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show abstract

“…An aptamer-based electrochemical biosensor for rapid Salmonella detection has been developed (Ma et al, 2014). Additional electrochemical biosensing strategies for Salmonella detection are based on the use of a screen-printed carbon electrode formed from nanoparticles (Noguera et al, 2011) or carbon nanotubes (Zelada-Guillen et al, 2010), resulting in a disposable immunosensor (Afonso et al, 2013).…”

Section: Salmonella Sppmentioning

confidence: 99%

Biosensors and their Applications in Food Safety: A Review

Yasmin¹,

Ahmed²,

Cho³

2016

Journal of Biosystems Engineering

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Background: Foodborne pathogens are a growing concern with respect to human illnesses and death. There is an increasing demand for improvements in global food safety. However, it is a challenge to detect and identify these harmful organisms in a rapid, responsive, suitable, and effective way. Results: Rapid developments in biosensor designs have contributed to the detection of foodborne pathogens and other microorganisms. Biosensors can automate this process and have the potential to enable fast analyses that are cost and time-effective. Various biosensor techniques are available that can identify foodborne pathogens and other health hazards. Conclusions: In this review, biosensor technology is briefly discussed, followed by a summary of foodborne pathogen detection using various transduction systems that exhibit specificity for particular foodborne pathogens. In addition, the recent application of biosensor technology to detect pesticides and heavy metals is briefly addressed.

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“…Hyeon et al [3] examined the potential of RNA aptamers in detecting Salmonella enteritidis and observed that the selected aptamer can specifically bind to Salmonella enteritidis without any cross-reactivity to other Salmonella serovars. Using a Salmonella-specific recognition aptamer, Ma et al [51] prepared an electrochemical biosensor for Salmonella detection, with a detection limit as low as 3 CFU/mL. Through ten rounds of Cell-SELEX, Park et al [52] identified single-stranded DNA (ssDNA) aptamers that can specifically bind to Salmonella enteritidis and Salmonella typhimurium, and the dissociation constants (K d ) were in the range of nanomolar to submicromolar levels.…”

Section: Microorganisms In Foodmentioning

confidence: 99%

Aptamer-Based Technology for Food Analysis

Liu

Zhang

2014

Appl Biochem Biotechnol

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Aptamers are short and functional single-stranded oligonucleotide sequences selected from systematic evolution of ligands by exponential enrichment (SELEX) process, which have the capacity to recognize various classes of target molecules with high affinity and specificity. Various analytical aptamers acquired by SELEX are widely used in many research fields, such as medicine, biology, and chemistry. However, the application of this innovative and emerging technology to food safety is just in infant stage. Food safety plays a very important role in our daily lives because varieties of poisonous and harmful substances in food affect human health. Aptamer technique is promising, which can overcome many disadvantages of existing detection methods in food safety, such as long detection time, low sensitivity, difficult, and expensive antibody preparation. This review provides an overview of various aptamer screening technologies and summarizes the recent applications of aptamers in food safety, and future prospects are also discussed.

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An aptamer-based electrochemical biosensor for the detection of Salmonella

Cited by 196 publications

References 26 publications

Aptamers against pathogenic microorganisms

Aptamers against pathogenic microorganisms

Biosensors and their Applications in Food Safety: A Review

Aptamer-Based Technology for Food Analysis

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