An aptamer-based dipstick assay for the rapid and simple detection of aflatoxin B1

Shim, Won‐Bo; Kim, Min Jin; Mun, Hyoyoung; Kim, Min-Gon

doi:10.1016/j.bios.2014.06.059

Cited by 189 publications

(77 citation statements)

References 22 publications

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“…Since the report of an aptamer for ochratoxin A (OTA) in 2008, 22 many aptasensors for OTA and AFB 1 have been developed for feed and food safety. [23][24][25][26][27][28][29] In our previous study, 27 an aptasensor based on qPCR was successfully designed for AFB 1 determination with high sensitivity. Aptasensors were developed for the detection of AFM 1 using electrochemical methods and impedance spectroscopy techniques.…”

Section: Introductionmentioning

confidence: 99%

A qPCR aptasensor for sensitive detection of aflatoxin M1

et al. 2016

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. In addition, the aptasensor developed here exhibits high selectivity for AFM 1 over other mycotoxins and small effects from cross-reaction with structural analogs. The method proposed here has been successfully applied to quantitative determination of AFM 1 in infant rice cereal and infant milk powder samples. Results demonstrated that the current approach is potentially useful for food safety analysis, and it could be extended to a large number of targets. IntroductionAflatoxin M 1 (AFM 1 ), one of the most toxic contaminants in dairy products, is a metabolite produced by dairy cows as a result of being fed with feeds contaminated with Aflatoxin B 1 (AFB 1 ). [3][4][5] Once present in dairy products, AFM 1 poses a hazard to humans (especially infants) who consume them. Results and Discussion Optimization of the amplification of complementary ssDNAThe complementary ssDNA is applied as the subsequent PCR template. The Optimization of streptavidin and the biotinylated aptamerOther factors would affect the performance of this method including the concentrations of streptavidin, the biotinylated aptamer and the complementary ssDNA and these all should be optimized. Under a fixed concentration of complementary ssDNA at 10 nM, the concentrations of streptavidin and the 6 biotinylated aptamer were analyzed for the PCR amplification signal change (Fig. S3).The adsorptive power of streptavidin to PCR tubes and the binding ability of streptavidin-coated tubes to biotinylated aptamer was primarily detected using different concentrations of streptavidin (Fig. S3). This also shows that there was a clear difference of Ct values between the control group and streptavidin-coated tubes, which showed the intense adsorptive ability between biotinylated aptamer and streptavidin-coated tubes. In analysis of the Ct values at different concentrations of streptavidin, the Ct value reached the lowest level when the concentration of streptavidin was 2.5 ng mL -1 . This graph also shows that the Ct values decreased with the increase of the amount of aptamer when aptamer levels were below 10.0 nM andalso that the Ct values increased with the amount of aptamer above 10.0 nM mainly because of steric hindrance. Thus, 2.5 ng mL -1 of streptavidin and 10 nM of aptamer were the optimal conditions for RT-qPCR amplification. AFM 1 determinationUnder optimal conditions, the amplification curves and calibration curve of this aptasensor for different levels of AFM 1 with AFM 1 DNA1 were determined by RT-qPCR (Fig. 1). As is shown in Fig. 1(A), the cycle number increased with an increase in AFM 1 . More complementary ssDNA would be released when more AFM 1 is present in the reaction system, which leads to a decrease in the amount of the PCR template and an increase in cycle number. Meanwhile, a good linear relationshipbetween Ct values and AFM 1 levels in the range of 1×10 -4 to 1 µg L -1 was obtained as (Table 1). Specificity analysisThe specificity of the aptasensor plays an important role in the development and practicality of this method. In ...

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Section: Introductionmentioning

confidence: 99%

A qPCR aptasensor for sensitive detection of aflatoxin M1

et al. 2016

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“…As another approach, from the illustrated figure 6, Lu and co-workers applied a strategy of AuNP networking in lateral flow aptasensor for adenosine detection This method inherently has same problem with sandwich format in the hybridization efficiency between aptamers and complemented DNA probes on test zone. To overcome this issue, the dipstick platform have been adopted in lateral flow aptasensors, which possessed two steps, pre-reaction and dipping a dipstick (Shim et al, 2014). In this method, aptamer was modified with biotin and complementary DNA (cDNA) was labelled with Cy5 dye.…”

Section: Lateral Flow Strip-based Aptasensorsmentioning

confidence: 99%

Aptamer-based nanobiosensors

Kim¹,

Raston

2016

Biosensors and Bioelectronics

355

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“…Bruno (2014) developed the sandwich format LFTSs for E. coli, L. monocytogenes, and Salmonella enterica by the use of DNA aptamers. Shim et al (2014) developed a LFTS for aflatoxin B1 (AFB1) detection based on an indirect competitive assay. A biotin-modified aptamer specific to AFB1 competes with a cy5-modified DNA probe to bind AFB1 in a sample in this assay.…”

Section: Recognition Elementsmentioning

confidence: 99%

A Review on Lateral Flow Test Strip for Food Safety

Kim¹,

Lim²,

Mo³

2015

Journal of Biosystems Engineering

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Background: Foodborne disease outbreaks from various food sources are a major health concern worldwide. Current methods for detection of foodborne pathogens are both expensive and time-consuming. Purpose: This review aims to present the current information available on the use of lateral flow test strips to detect pathogens in food products to enhance food safety. Results: Frequent foodborne disease outbreaks from various food sources have increased the need for rapid and easy methods for routine analysis of foodborne pathogens. Present detection methods for foodborne pathogens require expensive instruments, experts, and long time for sample analysis. Lateral flow test strips have drawn attention in recent years because of their ability to detect analytes quickly and easily. This review focuses on the principle of the lateral flow test, the various formats of lateral flow test strips, recognition elements, labeling tags, and reading instruments. In addition, this review also discusses the future prospects for the lateral flow test strips.

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An aptamer-based dipstick assay for the rapid and simple detection of aflatoxin B1

Cited by 189 publications

References 22 publications

A qPCR aptasensor for sensitive detection of aflatoxin M1

A qPCR aptasensor for sensitive detection of aflatoxin M1

Aptamer-based nanobiosensors

A Review on Lateral Flow Test Strip for Food Safety

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