An Aptamer-based Biosensor for Troponin I Detection in Diagnosis of Myocardial Infarction

Abstract

Background: Acute myocardial infarction (MI) accounts for one third of deaths. Cardiac troponin I (TnI) is a reliable biomarker of cardiac muscle tissue injury and is employed in the early diagnosis of MI. Objective:In this study, a molecular method is introduced to early diagnosis of MI by rapid detection of TnI. Materials and Methods: The detection method was based on electrochemical aptasensing, being developed using different methods and evaluation steps. A gold electrode was used as a transducer to successful immobilize 76base aptamer to fabricate a TnI biosensor. Results: The designed aptasensor could detect TnI in a range of 0.03 to 2.0 ng mL-1 without using any label, pre-concentration or amplification steps. The limit of detection was attained as 10 pg mL-1 without significant trouble of interfering species. The TnI biosensor demonestrated a stable, regenerative and reproducible function. 89 human samples were used to evaluate the performance of the TnI biosensor, and it represented 100% and 81%, diagnostic sensitivity and specificity, respectively. Conclusion: This aptasensor may be used as an applicable tool in the future of early medical diagnosis of MI.

Recent advances in cardiac biomarkers detection: From commercial devices to emerging technologies

“…Among them, acute myocardial infarction (AMI), acute coronary syndrome (ACS) and heart failure (HF) attract themost attention of scientists and clinicians [13]. Because of the very high frequency -one third of deaths [14,15] -of this pathology all over the world [2,7,8,12,[16][17][18][19][20][21], AMI is one of the WHO's major priorities [22]. Worldwide, between 5% and 20% of patients presenting to emergency departments (ED) do so with symptoms of myocardial infarction [23,24], which is defined as "cardiomyocyte necrosis in a clinical setting consistent with acute myocardial ischemia", according to the universal definition [25].…”

Electroanalysis on an Interdigitated Electrode for High-Affinity Cardiac Troponin I Biomarker Detection by Aptamer–Gold Conjugates

“… 4 , 5 Various sensing methods, such as surface plasmon resonance, optical sensing, enzyme-linked immunosorbent assay, and electrochemistry, have been used to detect cTnI. 6 − 9 Detection probes include antibodies, aptamers, DNA, and RNA, which are used to quantify the level of cTnI. 10 − 12 Among these probes, aptamers have received great attention from researchers due to their selective binding affinity to their targets.…”

“…In particular, aptamers are more attractive as biosensors because of their unique features, such as their high performance to bind to targets. 9 In this research, an aptamer generated against cTnI was used as the detection probe to diagnose MI on the IDE surface.…”

Antimicrobial Photothermal Treatment of Pseudomonas Aeruginosa by a Carbon Nanoparticles-Polypyrrole Nanocomposite

“…Nanomaterials are developed due to exhibition of novel characteristics arising from their size-dependent features, and widely used in medical diagnoses and therapies [8][9][10]. These materials have a usage in biological and medical applications such as labeling of biomolecules [11], drug and gene delivery [12][13][14], rapid and sensitive microbial detections [15,16], antimicrobial materials [10,[17][18][19], detection and recognition of DNA and proteins [20][21][22], probing of intrinsic DNA structure [23], biologic substitutes and regenerative medicine [24], hyperthermia [25], separation of specific cell types [26] and MRI contrast agents [27]. Photothermal agents employed in PTT should absorb NIR light, while they possess photostability with no cytotoxicity.…”