An Antivirally Active Sulfated Polysaccharide from Sargassum horneri (TURNER) C. AGARDH.

Hoshino, Tsuneo; Hayashi, Toshimitsu; Hayashi, Kyoko; Hamada, Jin; Lee, Jung‐Bum; Sankawa, Ushio

doi:10.1248/bpb.21.730

Cited by 119 publications

(72 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3) We have also isolated various sulfated polysaccharides showing antiviral activities. 4) Therefore, marine algae can be important resources in the discovery of antiviral compounds.…”

mentioning

confidence: 98%

Antiviral Sulfated Polysaccharide from Navicula directa, a Diatom Collected from Deep-Sea Water in Toyama Bay

Lee

Hayashi

Hirata

et al. 2006

Biological & Pharmaceutical Bulletin

Self Cite

128

View full text Add to dashboard Cite

A sulfated polysaccharide named naviculan was isolated from a diatom, Navicula directa (W. SMITH) RALFS, collected in deep sea water from Toyama Bay. The polysaccharide consisted of fucose, xylose, galactose, mannose, rhamnose and sulfate with an apparent molecular weight of 220000. It showed antiviral activities against herpes simplex viruses type 1 and 2, and influenza A virus with selectivity indices (CC 50 /IC 50 ) of 270, 510 and 32, respectively. Naviculan also showed an inhibitory effect on cell-cell fusion between CD4-expressing and human immunodeficiency virus (HIV) gp160-expressing cells that was used as a model system of infection with HIV.

show abstract

“…3) We have also isolated various sulfated polysaccharides showing antiviral activities. 4) Therefore, marine algae can be important resources in the discovery of antiviral compounds.…”

mentioning

confidence: 98%

Antiviral Sulfated Polysaccharide from Navicula directa, a Diatom Collected from Deep-Sea Water in Toyama Bay

Lee

Hayashi

Hirata

et al. 2006

Biological & Pharmaceutical Bulletin

Self Cite

128

View full text Add to dashboard Cite

show abstract

“…Many kinds of synthesized and algae-derived sulfated polysaccharides such as dextran sulfate, fucoidan, spirulan, and hornan have been reported to be antiviral components. [12][13][14][15][16][17][18][19] Some sulfated polysaccharides are known to inhibit both virus binding and penetration processes in the early phase of virus-cell interactions. In the present study, dextran sulfate was shown to have inhibitory effects on these two processes.…”

Section: Discussionmentioning

confidence: 99%

Anti-herpes Simplex Virus Target of an Acidic Polysaccharide, Nostoflan, from the Edible Blue-Green Alga Nostoc flagelliforme

Kanekiyo

Hayashi

Takenaka

et al. 2007

Biological & Pharmaceutical Bulletin

Self Cite

105

View full text Add to dashboard Cite

The acidic polysaccharide nostoflan was previously isolated as an antiviral component from the terrestrial alga Nostoc flagelliforme. In the present study, we examined the target for its anti-herpes simplex virus type 1 action. In time-of-addition experiments, the most sensitive stage of viral replication to nostoflan was found to be early events, including the virus binding and/or penetration processes. In order to determine what extent nostoflan may be involved in these processes, virus binding and penetration assays were separately performed. The results indicated that the inhibition of virus binding to but not penetration into host cells was responsible for the antiherpetic effect induced by nostoflan. Our study suggests that nostoflan may be a potential antiherpes agent.

show abstract

“…These findings suggested that the fucose residue had the b-L-configuration (an anomeric proton signal of a-L-fucose is observed at more than d 5.0). 20,22) C-4 and C-5 signals were observed in the 13 C-NMR spectrum at less than d 80. This finding suggested that component sugars possessed pyranoid forms (the chemical shifts of C-4 and C-5 for furanose forms are evident in the region d 80-85).…”

Section: -17)mentioning

confidence: 97%

Protective Effects of a Neutral Polysaccharide Isolated from the Mycelium of <i>Antrodia cinnamomea</i> on <i>Propionibacterium acnes</i> and Lipopolysaccharide Induced Hepatic Injury in Mice

Han

Nakamura

Zuo

et al. 2006

CHEMICAL & PHARMACEUTICAL BULLETIN

View full text Add to dashboard Cite

is a new species of the genus Antrodia (family polyporaceae, Aphyllophorales) that is parasitic on the inner cavity of the endemic species Cinnamomum kanehirai HAY. This endangered species has been used in Taiwan to treat food, alcohol, and drug intoxication, diarrhea, abdominal pain, hypertension, skin itching and liver cancer as a Chinese folk medicine. 1)The hot water extract of A. cinnamomea has hepatoprotective effect, 2,3) but the responsible component remains unclear. Here we describe the separation and purification of a neutral polysaccharide named ACN2a from a hot water extract of the mycelium of A. cinnamomea. We examined the hepatoprotective activity of the extract in mice with Propionibacterium acnes (P. acnes)-lipopolysaccharide (LPS) induced hepatotoxicity and elucidated the structure of this polysaccharide (ACN2a) by chemical and spectroscopic means. ExperimentalMaterials Antrodia cinnamomea mycelia (Lot No. #C082002-1) were provided by Simpson Biotech Co. Ltd. (Taiwan). Pullulans (Shodex Standard P-82) were purchased from Showa Denko Co. Ltd. (Tokyo, Japan) and used as standard molecular weight markers.General Optical rotation was determined in H 2 O using a JASCO DIP-360 automatic polarimeter and UV absorption was measured using a Shimadzu UV-2200 UV-VIS recording spectrometer. Infrared (IR) spectra were recorded in a KBr disk or liquid film using a JASCO FT/IR-230 IR spectrometer and NMR spectra were recorded on a Varian Unity Plus 500 ( 1 H, 500 MHz; Analysis by GC-MS proceeded on a Shimadzu GC-17A gas chromatograph equipped with a JEOL mass spectrometer. Samples were separated by thin-layer chromatography (TLC) on pre-coated silica-gel 60 F 254 and cellulose F plates (Merck Darmstadt Germany, 0.25, 0.1 mm, respectively), and spots were detected by spraying with 10% H 2 SO 4 or aniline hydrogen phthalate (AHP) and then heating at 100°C. Carbohydrate concentrations were determined using a phenol-H 2 SO 4 method. 4) Structural Analysis of a Neutral Polysaccharide from Antrodia cinnamomeaExtraction and Fractionation of Polysaccharides Freeze-dried mycelia of A. cinnamomea (1.5 kg) were extracted three times with 4 l of CHCl 3 at room temperature for 1 d, then combined extracts filtered and evaporated in vacuo to yield a CHCl 3 extract. The residue was dried and dipped into H 2 O (20 volumes) at room temperature for 1 h and extracted for 2 h 3 times at 100°C. The combined hot water extracts were concentrated to 800 and 3200 ml of EtOH was added. The mixture was stirred and left overnight in a refrigerator. The precipitates were filtered, washed with cold EtOH, dried and suspended in 10% trichloroacetic acid (TCA). The TCA-soluble fraction obtained by centrifugation (2500ϫg for 10 min) was dialyzed against distilled water for 3 d. The nondialyzable portion was lyophilized in vacuo to yield 14.25 g of a brownish residue (AC).Ion-Exchange Column Chromatography of AC AC (100 mg) dissolved in H 2 O was eluted through a column (Whatman International Ltd., Maidstone, England. 2.0ϫ20 cm) containing DE-52 ...

show abstract

An Antivirally Active Sulfated Polysaccharide from Sargassum horneri (TURNER) C. AGARDH.

Cited by 119 publications

References 1 publication

Antiviral Sulfated Polysaccharide from Navicula directa, a Diatom Collected from Deep-Sea Water in Toyama Bay

Antiviral Sulfated Polysaccharide from Navicula directa, a Diatom Collected from Deep-Sea Water in Toyama Bay

Anti-herpes Simplex Virus Target of an Acidic Polysaccharide, Nostoflan, from the Edible Blue-Green Alga Nostoc flagelliforme

Protective Effects of a Neutral Polysaccharide Isolated from the Mycelium of <i>Antrodia cinnamomea</i> on <i>Propionibacterium acnes</i> and Lipopolysaccharide Induced Hepatic Injury in Mice

Contact Info

Product

Resources

About