An antibody response to cholera toxin, but not soy proteins, following oral administration of adjuvanted soybean formulations

Abstract

In the present study, we questioned whether an oral formulation made from soybean seeds and containing the mucosal adjuvant, cholera toxin (CT), could induce any detectable antibody responses against soy proteins. Groups of mice were repeatedly gavaged with formulations containing high (15 mg) and low (1 mg) doses of soybean seed protein extracts, along with CT as an oral adjuvant. While robust mucosal and systemic antibody responses against CT were observed, no detectable IgA, IgM, IgG or IgE anti-soy protein antibodies could be found. Furthermore, mice in all treatment groups remained healthy and continued to gain weight over the course of the study. Collectively, these data demonstrate that oral soybean-based vaccine formulations do not induce detectable antibody responses to soy proteins, even when the formulations contain a potent mucosal adjuvant. These studies underscore the safety of oral vaccine formulations which might contain soy proteins as a component.

Allergen-specific immunoglobulin, histamine and T-cell responses induced by soybean glycinin and β-conglycinin in BALB/c mice of oral sensitisation

“…We acknowledged that the results are somewhat in conflict with Gizzarelli et al (2006) and Oakes et al (2009), who detected no allergen-specific response in mice immunised with soybean protein alone. The discrepancy is probably due to the differences in animal sensitisation procedures, as mice used in these experiments received allergens on separated days.…”

“…As a result, pigs (Hao, Zhan, Guo, Piao, & Li, 2009;Huang, Xu, Yu, Gao, & Liu, 2010;Sun, Li, Dong, Qiao, & Ma, 2008;, rats (Guo, Piao, Ou, Li, & Hao, 2007;Han, Ma, & Yin, 2010) and mice (Gizzarelli et al, 2006;Oakes, Piller, & Bost, 2009) have been used for study of allergic reactions to soybean allergens. However, to our knowledge, there are few reports available on allergenic effects of purified soybean glycinin and b-conglycinin in mice.…”