Allergen-specific immunoglobulin, histamine and T-cell responses induced by soybean glycinin and β-conglycinin in BALB/c mice of oral sensitisation

Abstract

2013) Allergenspecific immunoglobulin, histamine and T-cell responses induced by soybean glycinin and β-conglycinin in BALB/c mice of oral sensitisation, Food and Agricultural Immunology, 24:4, 489-501, Glycinin and b-conglycinin are major soybean allergens involved in food hypersensitivity. However, the mechanism of immune responses induced by glycinin and b-conglycinin has not been fully understood. Balb/c mice were oral sensitised with different doses (0.1, 1.0 and 10 mg/day) of soybean glycinin and b-conglycinin for five weeks. Allergen-specific immunoglobulin (Ig), serum histamine and T-cell responses were tested to assess the allergenic activity of glycinin and b-conglycinin. Mice sensitised with 0.1 or 1.0 mg/day allergens induced high levels of specific IgE, IgG1 and serum histamine compared with mice treated with saline. Furthermore, specific T-cell proliferation and significant up-regulation of interleukin (IL)-4, IL-5 and interferon (IFN)-g were observed in splenocytes from mice gavaged with 0.1 or 1.0 mg/day soybean proteins. Low doses of glycinin or b-conglycinin can induce allergic reactions in BALB/c mice, which might be associated with increased IgE and cytokine production.

Dynamics of Intestinal Inflammatory Cytokines and Tight Junction Proteins of Turbot (Scophthalmus maximus L.) During the Development and Recovery of Enteritis Induced by Dietary β-Conglycinin

“…NF-κB is considered to play an important role in regulating inflammatory response (Peng et al, 2018). In mice and piglets, the secretion of IFN-γ and IL-8 as well as the activation of NF-κB were remarkably induced by dietary 7S (Hao et al, 2009;Sun et al, 2013;Peng et al, 2018). Similar results were observed in the present study; the excessive expression of IL-1β, IL-8, IL-22, IFN-γ, and NF-κB p65 of turbot was induced by dietary 8% 7S in the first 3 weeks.…”

“…allergenic protein in soybean that favors the development of mammal enteritis (Lamia and Boye, 2007;Krishnan et al, 2009). In mice and piglets, the supplementation of 7S in diet inhibited the growth performance and impaired the histological structure and mucosal barrier of intestine (Guo et al, 2007;Liu et al, 2008;Hao et al, 2009;Xu et al, 2010;Sun et al, 2013;Zhao et al, 2014). However, very few studies are available regarding the effects of 7S on intestinal health of fish despite the widely reported studies with mammals.…”

Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies

“…T cell proliferation is one of the most common ways to assess T cell activation upon ex vivo re-stimulation with the allergen. To this end, several methods such as labeling of cells with a fluorescent dye (Pochard et al, 2010;Sun et al, 2016), incorporate a radioactive nucleoside into new strands of chromosomal DNA during mitotic cell division (Freidl et al, 2017;Stojadinovic et al, 2014) or colorimetric assays for assessing cell metabolic activity (Sun, Liu, Wang, Liu, & Feng, 2013;Wai, Leung, Leung, & Chu, 2016) have been used.…”

“…Proliferation assays have been performed for β-lactoglobulin (Mizumachi, Tsuji, & Kurisaki, 2008;Stojadinovic et al, 2014), ovalbumin (Castro et al, 2012;Sun et al, 2016), tropomyosin (Capobianco et al, 2008;Wai et al, 2016), Cyp c 1 from carp (Freidl et al, 2017), peanut (Pochard et al, 2010), cashew and walnut extract (Kulis, Pons, & Burks, 2009), and soybean proteins (Sun et al, 2013), showing in all the cases a strong proliferative response of the cells. Expression of specific surface markers have been measured in sensitized mice after challenge with whey and ovalbumin, showing an increase in the percentage of both, activated Th1 and Th2 cells compare to control animals (Lozano-Ojalvo, Perez-Rodriguez, Pablos-Tanarro, Molina, & Lopez-Fandino, 2017;Vonk et al, 2017).…”

“…Levels of secreted cytokines are often evaluated by ELISA or flow cytometry in cell culture supernatants. This method has been used to evaluate several food allergens from cow's milk (Kanjarawi et al, 2011;Stojadinovic et al, 2014), peanut (Smit et al, 2015;Zhu et al, 2016), hen's egg (Pablos-Tanarro, Lopez-Exposito, Lozano-Ojalvo, Lopez-Fandino, & Molina, 2016;Sun et al, 2016), soybeans (Sun et al, 2013) and shrimp (Wai et al, 2016). Moreover, extracts from whey, hen's egg white, peanut, cashew, walnut and sesame seeds have also been evaluated using this method (Lozano-Ojalvo et al, 2017;Smit et al, 2015;Vonk et al, 2017).…”