An antibiotic agent pyrrolo[1,2-a]pyrazine-1,4-dione,hexahydro isolated from a marine bacteria Bacillus tequilensis MSI45 effectively controls multi-drug resistant Staphylococcus aureus

Kiran, George Seghal; Priyadharsini, Sethu; Sajayan, Arya; Ravindran, Amrudha; Selvin, Joseph

doi:10.1039/c8ra00820e

Cited by 87 publications

(55 citation statements)

References 41 publications

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“…Furthermore, the isolated compound was non-cytotoxic towards mouse embryo fibroblast and non-haemolytic. Hence, compound (5) was recommended as an interesting compound that warrants further study considering its safety, non-haemolytic property as well as having dual role of reducing oxidative stress and inhibiting multidrug-resistant Staphylococcus aureus strains [87].…”

Section: Discussionmentioning

confidence: 99%

Streptomyces sp. Strain MUSC 125 from Mangrove Soil in Malaysia with Anti-MRSA, Anti-Biofilm and Antioxidant Activities

et al. 2020

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There is an urgent need to search for new antibiotics to counter the growing number of antibiotic-resistant bacterial strains, one of which is methicillin-resistant Staphylococcus aureus (MRSA). Herein, we report a Streptomyces sp. strain MUSC 125 from mangrove soil in Malaysia which was identified using 16S rRNA phylogenetic and phenotypic analysis. The methanolic extract of strain MUSC 125 showed anti-MRSA, anti-biofilm and antioxidant activities. Strain MUSC 125 was further screened for the presence of secondary metabolite biosynthetic genes. Our results indicated that both polyketide synthase (pks) gene clusters, pksI and pksII, were detected in strain MUSC 125 by PCR amplification. In addition, gas chromatography-mass spectroscopy (GC-MS) detected the presence of different chemicals in the methanolic extract. Based on the GC-MS analysis, eight known compounds were detected suggesting their contribution towards the anti-MRSA and anti-biofilm activities observed. Overall, the study bolsters the potential of strain MUSC 125 as a promising source of anti-MRSA and antibiofilm compounds and warrants further investigation.

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Section: Discussionmentioning

confidence: 99%

Streptomyces sp. Strain MUSC 125 from Mangrove Soil in Malaysia with Anti-MRSA, Anti-Biofilm and Antioxidant Activities

et al. 2020

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“…For this reason, GC-MS was used in the present study to detect chemical constituents in the methanolic extract MUSC 5 (Table 4 and Pyrrolopyrazines exist as a class of heterocyclic compounds frequently recovered from microbial extracts. For example, compound (1) isolated from a Bacillus strain was identified as the active compound with strong DPPH radical scavenging activity as well as exhibiting activity against multi-drug resistant Staphylococcus aureus [82] .Compound (2) among the 3 constituents present in ethyl acetate extract was identified in a marine Streptomyces sp. S2A as the major constituent responsible for the antioxidant activities (DPPH, ABTS, metal chelating and FRAP), antibacterial, enzyme inhibitory and cytotoxic effects [83] .…”

Section: Discussionmentioning

confidence: 99%

Streptomyces sp. strain MUSC 5 from mangrove forest in Malaysia: Identification, antioxidant potential and chemical profiling of its methanolic extract

Kemung¹,

Tan²,

Chan³

et al. 2020

Prog Micobes Mol Biol

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“…Several studies showed bioactivity from bioactive compound: 1-Naphthylbut-1-yn-3-one, Neophytadiene, 3-benzyl-1,4-diaza-2,5-dioxobicyclo, 13-Docosenamide, Stigmast-5-en-3-ol, I-nonadecene , oleic acid amide (Chajjed et al 2011;Singh et al 2012;Yousry et al 2009;Praddhessh et al 2017;Seanego and Roland (2012); Naragani et al 2016;Zaher et al 2015). On the other hand, pyrrolo [1,2-a] pyrazine-1,4-dione obtained from marine bacterial isolates effectively inhibits S. aureus (Kiran et al 2018), more than pyrrolo [1,2-a] pyrazine-1,4dione isolated from mangrove land found that Streptomyces mangrosivoli isolate can be used as an antioxidant agent (Ser et al 2015). Linolenic acid compounds are reported to have antioxidant abilities and Stigmasterol potential as antimicrobial and anticancer agents (Subaiyyan et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Characterization, identification, and analysis of bioactive compound of endophytic bacteria from Hoya multiflora Blume

2019

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Hoya multiflora Blume has been used for various purposes. Extract hoya has been reported to have bioactivity but extraction from the plant has been inefficient because it requires large biomass. Therefore, one of the appropriate ways to extract its bioactive compounds is using endophytic bacteria. The purposes of this research were to isolate, characterize, test its antimicrobial activity against two pathogenic bacteria and identify its bioactive compound. A total of 18 isolates from H. multiflora Blume were successfully obtained. Based on antimicrobial test, one isolate was able to inhibit Escherichia coli, one isolate inhibited Staphylococcus aureus and two isolates inhibited both of pathogenic bacteria. In addition, minimum inhibitory concentration (MIC) of ethyl acetate extract from HMB 1 was 62.5 ppm, while from HMD 4 was 125 ppm, both against E. coli and S. aureus. Based on 16S rRNA gene sequencing analysis, HMB 1 was the most similar (99.91%) with Bacillus siamensis KCTC-13613 and HMD 4 was the most similar (100%) with Bacillus aryabhattai B82W22. Using GC-MS, pyrollo[1,2-a]-pyrazine-1,4-dione was found to be dominant compound in the exctract bacteria of HMB 1and HMD 4 while Stigmasterol was found in extract HMD 4 and extract leaves H. multiflora Blume, respectively.

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An antibiotic agent pyrrolo[1,2-a]pyrazine-1,4-dione,hexahydro isolated from a marine bacteria Bacillus tequilensis MSI45 effectively controls multi-drug resistant Staphylococcus aureus

Abstract: A new antibiotic agent from sponge associated marine bacteria.

Cited by 87 publications

References 41 publications

Streptomyces sp. Strain MUSC 125 from Mangrove Soil in Malaysia with Anti-MRSA, Anti-Biofilm and Antioxidant Activities

Streptomyces sp. Strain MUSC 125 from Mangrove Soil in Malaysia with Anti-MRSA, Anti-Biofilm and Antioxidant Activities

Streptomyces sp. strain MUSC 5 from mangrove forest in Malaysia: Identification, antioxidant potential and chemical profiling of its methanolic extract

Characterization, identification, and analysis of bioactive compound of endophytic bacteria from Hoya multiflora Blume

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