An anisotropic nanocomposite hydrogel guides aligned orientation and enhances tenogenesis of human tendon stem/progenitor cells

Xu, Yingbin; Yin, Heyong; Chu, Jin; Eglin, David; Serra, Tiziano; Docheva, Denitsa

doi:10.1039/d0bm01127d

Cited by 30 publications

(31 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Alignment of fibrous ECM architecture is known to influence fibroblast spreading and polarization. For applications in tendon tissue engineering, alignment of tendon fibroblasts (tenocytes) within 3D hydrogels may be critical to mechanosensing and ECM deposition ( Schoenenberger et al, 2018 ; Xu et al, 2021 ). To enable fibroblast adhesion to magnetic fibers, residual VS groups were functionalized with the cell-adhesive peptide, CG RGD S, via Michael-type addition.…”

Section: Resultsmentioning

confidence: 99%

Magnetic Alignment of Electrospun Fiber Segments Within a Hydrogel Composite Guides Cell Spreading and Migration Phenotype Switching

Hiraki

Matera

Rose

et al. 2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Fibrous extracellular matrix (ECM) proteins provide mechanical structure and adhesive scaffolding to resident cells within stromal tissues. Aligned ECM fibers play an important role in directing morphogenetic processes, supporting mechanical loads, and facilitating cell migration. Various methods have been developed to align matrix fibers in purified biopolymer hydrogels, such as type I collagen, including flow-induced alignment, uniaxial tensile deformation, and magnetic particles. However, purified biopolymers have limited orthogonal tunability of biophysical cues including stiffness, fiber density, and fiber alignment. Here, we generate synthetic, cell-adhesive fiber segments of the same length-scale as stromal fibrous proteins through electrospinning. Superparamagnetic iron oxide nanoparticles (SPIONs) embedded in synthetic fiber segments enable magnetic field induced alignment of fibers within an amorphous bulk hydrogel. We find that SPION density and magnetic field strength jointly influence fiber alignment and identify conditions to control the degree of alignment. Tuning fiber length allowed the alignment of dense fibrous hydrogel composites without fiber entanglement or regional variation in the degree of alignment. Functionalization of fiber segments with cell adhesive peptides induced tendon fibroblasts to adopt a uniaxial morphology akin to within native tendon. Furthermore, we demonstrate the utility of this hydrogel composite to direct multicellular migration from MCF10A spheroids and find that fiber alignment prompts invading multicellular strands to separate into disconnected single cells and multicellular clusters. These magnetic fiber segments can be readily incorporated into other natural and synthetic hydrogels and aligned with inexpensive and easily accessible rare earth magnets, without the need for specialized equipment. 3D hydrogel composites where stiffness/crosslinking, fiber density, and fiber alignment can be orthogonally tuned may provide insights into morphogenetic and pathogenic processes that involve matrix fiber alignment and can enable systematic investigation of the individual contribution of each biophysical cue to cell behavior.

show abstract

Section: Resultsmentioning

confidence: 99%

Magnetic Alignment of Electrospun Fiber Segments Within a Hydrogel Composite Guides Cell Spreading and Migration Phenotype Switching

Hiraki

Matera

Rose

et al. 2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Tissue engineering practices have arisen as means to boost the engineering of tendon tissue. Diverse tissue engineering protocols including growth factors, scaffolds, cells, other bio-factors, and sometimes a mixture of them were adopted to improve the reconstruction of tendon tissues [ 394 , 395 ].…”

Section: Smart/stimuli-responsive Hydrogels Employed For Different Biomedical Applicationsmentioning

confidence: 99%

Smart/stimuli-responsive hydrogels: Cutting-edge platforms for tissue engineering and other biomedical applications

El-Husseiny

Mady

Hamabe

et al. 2022

Materials Today Bio

181

139

View full text Add to dashboard Cite

Recently, biomedicine and tissue regeneration have emerged as great advances that impacted the spectrum of healthcare. This left the door open for further improvement of their applications to revitalize the impaired tissues. Hence, restoring their functions. The implementation of therapeutic protocols that merge biomimetic scaffolds, bioactive molecules, and cells plays a pivotal role in this track. Smart/stimuli-responsive hydrogels are remarkable three-dimensional (3D) bioscaffolds intended for tissue engineering and other biomedical purposes. They can simulate the physicochemical, mechanical, and biological characters of the innate tissues. Also, they provide the aqueous conditions for cell growth, support 3D conformation, provide mechanical stability for the cells, and serve as potent delivery matrices for bioactive molecules. Many natural and artificial polymers were broadly utilized to design these intelligent platforms with novel advanced characteristics and tailored functionalities that fit such applications. In the present review, we highlighted the different types of smart/stimuli-responsive hydrogels with emphasis on their synthesis scheme. Besides, the mechanisms of their responsiveness to different stimuli were elaborated. Their potential for tissue engineering applications was discussed. Furthermore, their exploitation in other biomedical applications as targeted drug delivery, smart biosensors, actuators, 3D and 4D printing, and 3D cell culture were outlined. In addition, we threw light on smart self-healing hydrogels and their applications in biomedicine. Eventually, we presented their future perceptions in biomedical and tissue regeneration applications. Conclusively, current progress in the design of smart/stimuli-responsive hydrogels enhances their prospective to function as intelligent, and sophisticated systems in different biomedical applications.

show abstract

“…Besides tissue regeneration, CNTs have been applied on soft robots that mimic muscle and cartilage, through alignment and micropatterning [ 168 ]. Indeed, the anisotropic structure of CNTs can be beneficial to attain correct hierarchical organization to recapitulate natural tissues, which is an important aspect also for tendon regeneration [ 169 ]. The conductivity of CNTs can be exploited to this end also in the preparation of the hydrogel scaffold, for instance by using electrophoresis to align the CNTs within the biomaterial matrix [ 170 ].…”

Section: Recent Advancements On Hydrogels With Carbon Nanomaterials For Medicinementioning

confidence: 99%

Smart Hydrogels Meet Carbon Nanomaterials for New Frontiers in Medicine

2021

View full text Add to dashboard Cite

Carbon nanomaterials include diverse structures and morphologies, such as fullerenes, nano-onions, nanodots, nanodiamonds, nanohorns, nanotubes, and graphene-based materials. They have attracted great interest in medicine for their high innovative potential, owing to their unique electronic and mechanical properties. In this review, we describe the most recent advancements in their inclusion in hydrogels to yield smart systems that can respond to a variety of stimuli. In particular, we focus on graphene and carbon nanotubes, for applications that span from sensing and wearable electronics to drug delivery and tissue engineering.

show abstract

An anisotropic nanocomposite hydrogel guides aligned orientation and enhances tenogenesis of human tendon stem/progenitor cells

Abstract: An anisotropic magnetically-responsive collagen hydrogel loaded with iron oxide nanoparticles augments aligned cell row formation and tenogenic gene expression of tendon stem/progenitor cells.

Cited by 30 publications

References 39 publications

Magnetic Alignment of Electrospun Fiber Segments Within a Hydrogel Composite Guides Cell Spreading and Migration Phenotype Switching

Magnetic Alignment of Electrospun Fiber Segments Within a Hydrogel Composite Guides Cell Spreading and Migration Phenotype Switching

Smart/stimuli-responsive hydrogels: Cutting-edge platforms for tissue engineering and other biomedical applications

Smart Hydrogels Meet Carbon Nanomaterials for New Frontiers in Medicine

Contact Info

Product

Resources

About