An animal model in sheep for biocompatibility testing of biomaterials in cancellous bones

Nuss, Katja; Auer, Joerg A.; Boos, Alois; Rechenberg, Brigitte von

doi:10.1186/1471-2474-7-67

Cited by 81 publications

(83 citation statements)

References 43 publications

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“…The results in small animals such as rodents have to be considered with caution if we want to extrapolate them to clinical applications and need, at least, to be confirmed by studies in large animals. The sheep is often chosen as an experimental animal because its bone structure and bone remodelling rate are very similar to those of humans [13].…”

Section: Introductionmentioning

confidence: 99%

Augmentation of bone defect healing using a new biocomposite scaffold: An in vivo study in sheep

et al. 2010

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a b s t r a c tPrevious studies support resorbable biocomposites made of poly(L-lactic acid) (PLA) and b-tricalcium phosphate (TCP) produced by supercritical gas foaming as a suitable scaffold for tissue engineering. The present study was undertaken to demonstrate the biocompatibility and osteoconductive properties of such a scaffold in a large animal cancellous bone model. The biocomposite (PLA/TCP) was compared with a currently used b-TCP bone substitute (ChronOS™, Dr. Robert Mathys Foundation), representing a positive control, and empty defects, representing a negative control. Ten defects were created in sheep cancellous bone, three in the distal femur and two in the proximal tibia of each hind limb, with diameters of 5 mm and depths of 15 mm. New bone in-growth (osteoconductivity) and biocompatibility were evaluated using microcomputed tomography and histology at 2, 4 and 12 months after surgery. The in vivo study was validated by the positive control (good bone formation with ChronOS™) and the negative control (no healing with the empty defect). A major finding of this study was incorporation of the biocomposite in bone after 12 months. Bone in-growth was observed in the biocomposite scaffold, including its central part. Despite initial fibrous tissue formation observed at 2 and 4 months, but not at 12 months, this initial fibrous tissue does not preclude long-term application of the biocomposite, as demonstrated by its osteointegration after 12 months, as well as the absence of chronic or long-term inflammation at this time point.

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Section: Introductionmentioning

confidence: 99%

Augmentation of bone defect healing using a new biocomposite scaffold: An in vivo study in sheep

et al. 2010

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“…This study contributes information that is extremely relevant to the use of LBG, produced according to the protocol developed by the main author, in humans, since it demonstrates its osseointegration. Previous studies endorse the use of this LBG both in animals (15,16) and in humans (2,5,18) . Meyer et al (21) brought forth the first data relating to histological osseointegration in relation to LBGTutobone ® -in humans.…”

Section: Discussionmentioning

confidence: 99%

“…Our study is justified since there is little detailed information on the subject and the available data are controversial (17)(18)(19)(20)(21)(22) .…”

Section: Introductionmentioning

confidence: 99%

Enxerto bovino liofilizado: comportamento histológico após seguimento de 49 meses em seres humanos

Gália

Júnior²,

Ávila³

et al. 2012

Rev. bras. ortop.

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Objective: To analyze the histological behavior of bovine lyophilized grafts (BLG) produced according to a protocol developed by the first author, in humans over a 49-month period by measuring the graft/bone neoformation ratio in relation to the total mineralized area. Methods: This was a case series involving 12 patients: eight females (66%) and four males (34%), totaling 13 biopsies. BLG was used, and surgical reintervention was subsequently required during the period 2000 to 2011. The slides produced were stained with hematoxylin-eosin (HE), were analyzed by a pathologist and were digitized for the proposed evaluation. Results: The mean age was 57 years and the mean follow-up was 49 months (range: 6-115). The average proportion of BLG was 42% (range: 13-85) and neoformed bone, 58% (range: 15-87) in relation to the total area mineralized. Conclusions: This study demonstrated that the BLG used presented osteoconductive characteristics and biocompatibility. BLG is a therapeutic option that can be used in orthopedic surgery in which bone defects need to be filled.

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“…This model has previously been used to investigate the osseointegration properties of various implant designs [9]. The ovine model is well established in orthopaedic and biomedical research [1][2][3][4][5]10]. The similarity in lamellar bone structure to humans has made this model common in research involving fracture healing, osteosynthesis techniques and implant osseointegration [3,21,22].…”

Section: Discussionmentioning

confidence: 99%

“…Numerous animal models have been described and utilized in literature. These included: pig, dog, sheep, goat, rat, guinea pig, chicken, and mice [2][3][4][5][6][7]. These animal models differ with respect to: anatomical limitations, biologic acceptability of certain materials, availability of the animals, and ethical considerations.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Various Positions on the Ovine Pelvis for Dental Implant Research

Kloppers¹

2016

IJDOS

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Aims: An ex vivo and in vivo study to determine various positions in the ovine pelvis to evaluate dental implant placement. Settings and Design:• A novel, unique surgical dissection was described as the caudal roll-up technique and was utilized to obtain maximal exposure to the Linea Glutea, with the least morbidity.• Insertion torque (IT) of osseo-integrated dental implants was measured at predetermined positions described as A-F along the ovine linea glutea.• The amount of outer cortical bone was measured at predetermined sections A-F on harvested ovine pelvi, to coincide with the implant positions as placed. Statistical analysis used: Descriptive Study.Intraoral dental implant research in animals is challenging, owing in particular to different tooth morphology. One of the most challenging problems with intraoral implant research in animals is an infection form contaminated oral cavity, which is evident in intraoral research related to pigs and dogs [8]. Intraoral dental implant studies by Vlaminck confirmed that the specific oral biomechanics inherent to a sheep's constant ruminant activity accounted for high degrees of implant failures [13,25]. A novel, unique surgical dissection was described as the caudal roll-up technique and was utilized to obtain maximal exposure to the Linea Glutea, with the least morbidity. Data collected concerning the insertion torque of implants placed and the cortical thickness measurements of the sheep's ilia was discussed.

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An animal model in sheep for biocompatibility testing of biomaterials in cancellous bones

Cited by 81 publications

References 43 publications

Augmentation of bone defect healing using a new biocomposite scaffold: An in vivo study in sheep

Augmentation of bone defect healing using a new biocomposite scaffold: An in vivo study in sheep

Enxerto bovino liofilizado: comportamento histológico após seguimento de 49 meses em seres humanos

Evaluation of Various Positions on the Ovine Pelvis for Dental Implant Research

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