Enxerto bovino liofilizado: comportamento histológico após seguimento de 49 meses em seres humanos

Abstract

Objective: To analyze the histological behavior of bovine lyophilized grafts (BLG) produced according to a protocol developed by the first author, in humans over a 49-month period by measuring the graft/bone neoformation ratio in relation to the total mineralized area. Methods: This was a case series involving 12 patients: eight females (66%) and four males (34%), totaling 13 biopsies. BLG was used, and surgical reintervention was subsequently required during the period 2000 to 2011. The slides produced were stained with hematoxylin-eosin (HE), were analyzed by a pathologist and were digitized for the proposed evaluation. Results: The mean age was 57 years and the mean follow-up was 49 months (range: 6-115). The average proportion of BLG was 42% (range: 13-85) and neoformed bone, 58% (range: 15-87) in relation to the total area mineralized. Conclusions: This study demonstrated that the BLG used presented osteoconductive characteristics and biocompatibility. BLG is a therapeutic option that can be used in orthopedic surgery in which bone defects need to be filled.

Antimicrobial Activity of Bovine Bone Scaffolds Impregnated with Silver Nanoparticles on New Delhi Metallo-β-Lactamase-Producing Gram-Negative Bacilli Biofilms

“…The discs of 6 mm in diameter and 3 mm thick (0.084 mL) (retrieved from a sagittal cut in the bone using a table band saw, using 6 mm diamond drill and small bone cylinders) (Figure 2) were made. Then, using a 1 2 inch chisel, a hammer and a plastic caliper, the discs were measured and cut and processed using a modified, previously described protocol [16]. The procedure used to AgNPs synthesis was adapted from Dong et al [17], where 8.5 mg of silver nitrate was dissolved (AgNO 3 ) in 45 mL of deionized water, followed by the addition of 14.7 mg of trisodium citrate (Na 3 C 6 H 5 O 7 ).…”

“…Heterologous grafting is an option that has been widely used in the most varied areas of medicine. Its main representative is the bovine graft, which is easy to obtain, has great availability and is like human bone [1,2]. However, it may present limitations related to antigenicity [3].…”

“…Bovine bone has very similar chemical (composition), physical (porosity and size) characteristics and biological behavior to human bone, which favors osteoconduction. In addition, its inorganic matrix provides calcium and phosphorus content, which are essential to the formation of new bone tissue at the implant site [1,4].…”

Antimicrobial Activity of Bovine Bone Scaffolds Impregnated with Silver Nanoparticles on New-Delhi-Metallo-β-Lactamase Producing <em>Enterobacterales</em> Biofilms

“…Heterologous grafting is an option that has been widely used in the most varied areas of medicine. Its main representative is the bovine graft, which is easy to obtain, has great availability and is similar to human bone [5,6]. However, it may present limitations related to antigenicity [7].…”

“…Bovine bone has chemical (composition), physical (porosity and size) characteristics and biological behavior very similar to human bone, which favors osteoconduction. In addition, its inorganic matrix provides calcium and phosphorus content, which are essential for the formation of new bone tissue at the implant site [5,8].…”