An androgen-regulated miRNA suppresses Bak1 expression and induces androgen-independent growth of prostate cancer cells

Shi, Xu; Xue, Leigang; Yang, Joy C.; Hong, Ai; Zhao, Jianjun; Ma, Xu; Tepper, Clifford G.; Evans, Christopher P.; Kung, Hsing Jien; White, Ralph W. deVere

doi:10.1073/pnas.0706641104

Cited by 421 publications

(443 citation statements)

References 41 publications

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“…2a) to analyse the role of miRNA in prostate cancer progression. In LNCaP cells, a total of 44 miRNAs were significantly induced by DHT (41.2-fold), including representative AR-regulated miRNAs such as miR-125b 24 , miR-21 (ref. 25) and miR-148a 26 , while 30 miRNAs were induced by DHT (41.2-fold) in BicR cells.…”

Section: Resultsmentioning

confidence: 99%

TET2 repression by androgen hormone regulates global hydroxymethylation status and prostate cancer progression

et al. 2015

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Modulation of epigenetic patterns has promising efficacy for treating cancer. 5-Hydroxymethylated cytosine (5-hmC) is an epigenetic mark potentially important in cancer. Here we report that 5-hmC is an epigenetic hallmark of prostate cancer (PCa) progression. A member of the ten-eleven translocation (TET) proteins, which catalyse the oxidation of methylated cytosine (5-mC) to 5-hmC, TET2, is repressed by androgens in PCa. Androgen receptor (AR)-mediated induction of the miR-29 family, which targets TET2, are markedly enhanced in hormone refractory PCa (HRPC) and its high expression predicts poor outcome of PCa patients. Furthermore, decreased expression of miR-29b results in reduced tumour growth and increased TET2 expression in an animal model of HRPC. Interestingly, global 5-hmC modification regulated by miR-29b represses FOXA1 activity. A reduction in 5-hmC activates PCa-related key pathways such as mTOR and AR. Thus, DNA modification directly links the TET2-dependent epigenetic pathway regulated by AR to 5-hmC-mediated tumour progression.

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Section: Resultsmentioning

confidence: 99%

TET2 repression by androgen hormone regulates global hydroxymethylation status and prostate cancer progression

et al. 2015

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“…miRNA profiling has been recently performed in various malignancies, including prostate cancer. One of the miRNAs characteristic of prostate cancer is miR125b, 18 which we used as a positive control. miR-125b is upregulated in prostate cancer and confirmed increased expression of one of these targets, EIF4EBP1, in prostate cancer tissues.…”

Section: Discussionmentioning

confidence: 99%

“…Among the short RNAs, we focused on the miRNAs with known sequences based on the search by miRBase 9.1. miRNAs in a list of the 10 highest frequencies were selected and the androgen responsiveness (41.5-fold over basal) of the 10 miRNAs was validated by qPCR, using miR-125b as a positive control (Figure 1). 18 miR-141, miR-200a, and miR-148a promote LNCaP cell growth…”

Section: Identification Of Androgen-responsive Mirnasmentioning

confidence: 99%

miR-148a is an androgen-responsive microRNA that promotes LNCaP prostate cell growth by repressing its target CAND1 expression

Murata

Takayama

Katayama

et al. 2010

Prostate Cancer Prostatic Dis

129

105

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Recent advances in cancer biology reveal that microRNAs (miRNAs) are involved in the regulation of cancer-related genes, or they function as tumor suppressors or oncogenes. In prostate cancer, evidence has accumulated for the contribution of the androgen-dependent gene network to tumor growth, although the precise functions of miRNAs in prostate cancer remain to be investigated. Here, we identified androgen-responsive miRNAs by the short RNA sequencing analysis in LNCaP prostate cancer cells. Among 10 miRNAs with known sequences, we have determined that miR148a reduces the expression of cullin-associated and neddylation-dissociated 1 (CAND1), a negative regulator of SKP1-Cullin1-F-box (SCF) ubiquitin ligases, by binding to the 3 0 -untranslated region of CAND1 mRNA. CAND1 knockdown by small interfering RNA promoted the proliferation of LNCaP cells. Our study indicates the potential contribution of miR-148a to the growth of human prostate cancer.

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“…Several microRNAs (miRNAs) are now associated with the progression and classification of this and other malignancies (Lu et al, 2005;Volinia et al, 2006;Porkka et al, 2007;Shi et al, 2007;Ozen et al, 2008;Prueitt et al, 2008;Tong et al, 2009). Evidence is accumulating that small nuclear RNA and small nucleolar RNA (snoRNA) are also employed in cis and trans gene regulation and alternative splicing besides their established function in ribosomal RNA modification (Reis et al, 2004;Mattick and Makunin, 2006;Dong et al, 2008;Ender et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Diagnostic and prognostic signatures from the small non-coding RNA transcriptome in prostate cancer

et al. 2011

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Prostate cancer (PCa) is the most frequent male malignancy and the second most common cause of cancer-related death in Western countries. Current clinical and pathological methods are limited in the prediction of postoperative outcome. It is becoming increasingly evident that small non-coding RNA (ncRNA) species are associated with the development and progression of this malignancy. To assess the diversity and abundance of small ncRNAs in PCa, we analyzed the composition of the entire small transcriptome by Illumina/ Solexa deep sequencing. We further analyzed the micro-RNA (miRNA) expression signatures of 102 fresh-frozen patient samples during PCa progression by miRNA microarrays. Both platforms were cross-validated by quantitative reverse transcriptase-PCR. Besides the altered expression of several miRNAs, our deep sequencing analyses revealed strong differential expression of small nucleolar RNAs (snoRNAs) and transfer RNAs (tRNAs). From microarray analysis, we derived a miRNA diagnostic classifier that accurately distinguishes normal from cancer samples. Furthermore, we were able to construct a PCa prognostic predictor that independently forecasts postoperative outcome. Importantly, the majority of miRNAs included in the predictor also exhibit high sequence counts and concordant differential expression in Illumina PCa samples, supported by quantitative reverse transcriptase-PCR. Our findings provide miRNA expression signatures that may serve as an accurate tool for the diagnosis and prognosis of PCa.

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An androgen-regulated miRNA suppresses Bak1 expression and induces androgen-independent growth of prostate cancer cells

Cited by 421 publications

References 41 publications

TET2 repression by androgen hormone regulates global hydroxymethylation status and prostate cancer progression

TET2 repression by androgen hormone regulates global hydroxymethylation status and prostate cancer progression

miR-148a is an androgen-responsive microRNA that promotes LNCaP prostate cell growth by repressing its target CAND1 expression

Diagnostic and prognostic signatures from the small non-coding RNA transcriptome in prostate cancer

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