An analytical method for the quantitative determination of iron ion chelating capacity: development and validation

Abstract

Iron is a fundamental microelement for human life; however, deficiencies or excesses of these metal ions can cause severe complications and mortality. Chelators are compounds that bind and inhibit iron. Ultraviolet-visible (UV-vis) spectrophotometric methods are key analytical tools in the identification of chemical entities, with the benefits of having good precision and accuracy, and the equipment being easily available as well as quick and simple to implement. In this study, we aimed to provide an alternative, cheaper method for the quantification of iron ion chelation by substituting ferrozine for gallic acid and validating its use with UV-vis according to official ANVISA and ICH guidelines. The parameters assessed were specificity, linearity, precision, accuracy, robustness, and finally, the percentage of iron ions chelating was calculated. The results demonstrated that this method was accurate, simple, specific, selective, precise, and reproducible, and was successfully validated for the determination of iron ions chelating. The percentage of iron ions chelating, promoted by the standard chelator EDTA, was 45% and 47% for Fe2+ and Fe3+, respectively. It is concluded that this new method is beneficial in terms of its simplicity, rapidness, low cost, and the fact that it produces very low levels of dangerous residues.

Rapid Method for Quantification of Iron (Fe+3) from Ferrazone (NaFe-EDTA) in Fortified Wheat Flour

“…These results were consistent with those reported by Harke et al 28 who found 78–88% repeatability in results during Fe +3 detection in food samples using biogenic silver nanoparticles. A similarity index was observed between the current findings and those of da Silva Santos et al, 31 where <5 RSD % in intraday results was obtained during quantification of Fe ion chelating capacity using a newly developed analytical method.…”

“…Values for relative standard deviation (%) are displayed in Table 3 . The percent recovery of 100.8, 103, and 104 for each level showed a close resemblance with the recoveries (%) stated by da Silva Santos et al 31 obtained at three concentrations (6.2, 50, and 200 μg/mL) during analytical method validation of iron ion chelating quantification with EDTA. Inter-person precision results were agreed with the findings of da Silva Santos et al, 31 who reported ruggedness RSD value at <5% during his research work on method validation of iron ion estimation.…”

“…The percent recovery of 100.8, 103, and 104 for each level showed a close resemblance with the recoveries (%) stated by da Silva Santos et al 31 obtained at three concentrations (6.2, 50, and 200 μg/mL) during analytical method validation of iron ion chelating quantification with EDTA. Inter-person precision results were agreed with the findings of da Silva Santos et al, 31 who reported ruggedness RSD value at <5% during his research work on method validation of iron ion estimation. While investigating the efficacy of two separate analytical methods for simultaneous determination of DS and DC, Mallick et al 32 calculated the ruggedness value of 0.95% for DS and 0.96% for DC, showing the acceptability between the two methods.…”

“…Inter-assay precision was found in the range of 74–94% by Harke et al 28 during their experiments on different food samples for the detection of Fe +3 using novel biogenic techniques. A similar range of inter-day precision with <5 RSD% was reported by da Silva Santos et al, 31 when they developed and validated a rapid quantitative assay for determination of iron ion chelating capacity.…”

Generating signals at converging liquid fronts to create line-format readouts of soluble assay products in three-dimensional paper-based devices

“…47,48 We minimized the background signal by incorporating ethylenediaminetetraacetic acid (EDTA) in the ferrozine side of the sample distribution layer. 49,50 We tested both the effect of different EDTA and ferrozine concentrations on the signal to background ratio (Tables S5 and S6 †). As the concentration of ferrozine increased, the intensities of both the signal formed at the readout zone and the background increased.…”