An analytical approach to profile steroidal saponins in food supplements: The case of Ruscus aculeatus

Mari, Angela; Napolitano, Assunta; Perrone, Angela; Pìzza, Cosimo; Piacente, Sonia

doi:10.1016/j.foodchem.2012.02.099

Cited by 14 publications

(13 citation statements)

References 19 publications

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“…Synthesized in 26.8% overall yield (Wei & Du, 2012) Steroidal saponins from Ruscus aculeatus (Liliaceae) TOF (CHCA) To develop HPLC-ESI/ITMS analytical method (Mari, et al, 2012) Steviobioside (steroid), from Stevia rebaudiana…”

Section: Medical Applicationsmentioning

confidence: 99%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2011–2012

Harvey

2015

Mass Spectrometry Reviews

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This review is the seventh update of the original article published in 1999 on the application of MALDI mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2012. General aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, and fragmentation are covered in the first part of the review and applications to various structural types constitute the remainder. The main groups of compound are oligo- and poly-saccharides, glycoproteins, glycolipids, glycosides, and biopharmaceuticals. Much of this material is presented in tabular form. Also discussed are medical and industrial applications of the technique, studies of enzyme reactions, and applications to chemical synthesis. © 2015 Wiley Periodicals, Inc. Mass Spec Rev 36:255-422, 2017.

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Section: Medical Applicationsmentioning

confidence: 99%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2011–2012

Harvey

2015

Mass Spectrometry Reviews

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“…Extracts from the rhizome have been used in many dietary supplements (Mari et al . ; Di Novella et al . ; Di Sanzo et al .…”

Section: Conservationmentioning

confidence: 99%

“…It has indeed been used as a 'butcher's broom' to clean chopping blocks (Pedersen 1994), also to make garden besoms (Bean 1980;Kızılarslan & € Ozhatay 2012) and brooms for removing embers from bread ovens in southern Italy (Salerno, Guarrera & Caneva 2005). Extracts from the rhizome have been used in many dietary supplements (Mari et al 2012;Di Novella et al 2013;Di Sanzo et al 2013;Gonz alez et al 2013), for treating warts, chilblains, piles and minor pains (Guarrera 2005;Chauhan, Ruby & Dwivedi 2012;Akyol & Altan 2013), and more recent medicinal uses described in VI (F). R. aculeatus shoots are eaten boiled or fried in spring in Sicily and Italy (Corsi & Pagni 1979;Paoletti, Dreon & Lorenzoni 1995;Lentini & Venza 2007;Laghetti et al 2011;di Tizio et al 2012).…”

Section: Conservationmentioning

confidence: 99%

Biological Flora of the British Isles: Ruscus aculeatus

Thomas

Mukassabi

2014

Journal of Ecology

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Summary1. This account presents information on all aspects of the biology of Ruscus aculeatus L. (Butcher's broom) that are relevant to understanding its ecological characteristics and behaviour. The main topics are presented within the standard framework of the Biological Flora of the British Isles: distribution, habitat, communities, responses to biotic factors, responses to environment, structure and physiology, phenology, floral and seed characters, herbivores and disease, history and conservation. 2. Ruscus aculeatus is a multistemmed monocotyledonous shrub with leaves functionally replaced by cladodes and photosynthetic stems. It is native to southern England primarily in dry shaded woodland and hedgerows (but widely planted elsewhere) often, but not exclusively, on base-rich soil. It is rarely abundant in any habitat, usually forming widely spread discrete clumps. 3. Ruscus aculeatus is remarkably shade tolerant and drought resistant with low water conductance and transpiration, and water storage in the cladodes. Yet unusually for a drought-tolerant stemphotosynthetic plant, it prefers shady environments. 4. The flowers have few if any pollinating mechanisms, low seed production and fruit/seed dispersal are largely ineffective, which may be a relict of its evolution in a tropical Tertiary climate. Population survival primarily depends upon vegetative spread from stout rhizomes, aided by the plant's general unpalatability. 5. Over-collecting for medicinal steroidal saponins has caused some population declines, particularly in eastern Europe, but it is otherwise facing few conservation problems.

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“…Saponin molecules are composed of a sugar (glycone) and a non-sugar part (aglycone), which are connected to each other with glycosidic bonds. Studies have shown that the pharmacological effect of the Ruscus saponins increases with the decrease of the number of the sugar residues [26][27][28]. Ruscoside can hydrolyze into its derivatives with two alternative methods: a chemical approach based on acid hydrolysis and biotechnological approach comprise enzyme application.…”

mentioning

confidence: 99%

Synthesis of alginate‐silica hybrid hydrogel for biocatalytic conversion by β‐glucosidase in microreactor

Onbas

Yeşil-Çeliktaş

2018

Engineering in Life Sciences

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The organic–inorganic hybrid materials have been used in different fields to immobilize biomolecules since they offer many advantages. The aim of this study was to optimize and characterize the alginate‐silica hybrid hydrogel as a stable and injectable form for microfluidic systems using internal gelation method and increase the stability and activity of immobilized enzyme for biocatalytic conversions as well. Characterization was carried out by scanning electron microscopy, energy dispersive spectroscopy/mapping, Brunauer–Emmett–Teller, Barrett–Joyner–Halenda, and Fourier‐transform infrared spectroscopy analyses, and the shrinkages of monoliths were evaluated. Subsequent to optimizing the enzyme concentration (40 μg), hydrolytic conversion of 4‐nitrophenyl β‐d‐glucopyranoside (pNPG) was performed to understand the behavior of the bioconversion in the microfluidic system. The yield was 94% which reached the equilibrium at 24 h indicating that the alginate‐silica gel derived microsystem overcome some drawbacks of monolithic systems. Additionally, bioconversion of Ruscus aculeatus saponins was carried out at the same setup in order to obtain aglycon part, which has pharmaceutical significance. Although pure aglycon could not be achieved, an intermediate compound was obtained based on the HPLC analysis. The developed formulation can be utilized for various life science applications.

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An analytical approach to profile steroidal saponins in food supplements: The case of Ruscus aculeatus

Cited by 14 publications

References 19 publications

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2011–2012

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2011–2012

Biological Flora of the British Isles: Ruscus aculeatus

Synthesis of alginate‐silica hybrid hydrogel for biocatalytic conversion by β‐glucosidase in microreactor

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