An analysis of suppressing migratory effect on human urinary bladder cancer cell line by silencing of snail-1

Salehi, Shima; Mansoori, Behzad; Mohammadi, Ali; Davoudian, Sadaf; Shenas, Seyed Mohammad Hossein Musavi; Shajari, Neda; Majidi, Jafar; Baradaran, Behzad

doi:10.1016/j.biopha.2017.10.044

Cited by 11 publications

(10 citation statements)

References 16 publications

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“…Multivariate Cox analysis indicated that Snail is an independent prognostic factor for ESCC. This is consistent with the results of several previous studies (35,36).…”

Section: Os Pfs -----------------------------------------------------supporting

confidence: 94%

“…In studies conducted by Salehi et al (35) and Shenas et al (36), when Snail was silenced, the expression of vimentin was decreased, E-cadherin was induced and EMT was inhibited, and Snail was indicated to play a crucial role in the survival of bladder cancer. The high expression of Snail has also been reported to indicate a poor prognosis in patients with infiltrative breast lesions (37).…”

Section: Os Pfs -----------------------------------------------------mentioning

confidence: 99%

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Expression of Twist, Slug and Snail in esophageal squamous cell carcinoma and their prognostic significance

Zhou

Biekemitoufu

et al. 2021

Oncol Lett

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Esophageal cancer is one of the most common types of malignancy worldwide. At present, surgical resection is the main treatment for esophageal cancer, but recurrence and distant metastasis are the main causes of mortality. The transcription factors Twist, Slug and Snail regulate epithelial-mesenchymal transition and thereby participate in tumor invasion and metastasis. The aim of the present study was to investigate the expression of Twist, Slug and Snail in esophageal squamous cell carcinoma (ESCC) and their prognostic significance. The expression of Twist, Slug and Snail in 229 paraffin-embedded ESCC and matched normal mucosal tissues was detected by immunohistochemistry. The expression differences of Twist, Slug and Snail in the ESCC and normal tissues were compared by χ 2 test, and the associations between the three proteins and the clinicopathological parameters of ESCC were analyzed. The expression levels of Twist, Slug and Snail in 29 fresh frozen ESCC and matched normal mucosal tissues were detected by reverse transcription-quantitative PCR. The correlations among Twist, Slug and Snail in ESCC were examined by Pearson's correlation analyses. In addition, single factor and multivariate Cox regression analyses were used to analyze the influence of Twist, Slug and Snail on the prognosis of ESCC. Twist was found to be highly expressed in ESCC. The difference of Slug expression in ESCC was associated with differentiation degree, TNM stage and vascular invasion, but no significant association was observed between Snail expression and any clinicopathological parameters. In ESCC, there were significant differences in protein expression between Twist and Snail, and Slug and Snail. The mRNA expression level of Twist in ESCC was significantly higher than that in normal esophageal mucosa. However, the mRNA expression of Slug in normal esophageal mucosa was higher than that in ESCC, and the mRNA expression levels of Twist and Snail were positively correlated in ESCC. Kaplan-Meier analysis of 229 patients with ESCC revealed that Snail influenced the overall survival, as did the co-expression of Twist and Snail. Nerve invasion was also identified as an independent factor affecting the progression-free survival of ESCC. The results indicate that Twist is highly expressed, Slug may be a tumor suppressor, and Snail is an independent prognostic factor in ESCC. Twist and Snail are positively correlated, and the simultaneous inhibition of Twist and Snail protein expression may be beneficial for prolonging the overall survival of patients with ESCC.

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“…Multivariate Cox analysis indicated that Snail is an independent prognostic factor for ESCC. This is consistent with the results of several previous studies (35,36).…”

Section: Os Pfs -----------------------------------------------------supporting

confidence: 94%

Section: Os Pfs -----------------------------------------------------mentioning

confidence: 99%

Expression of Twist, Slug and Snail in esophageal squamous cell carcinoma and their prognostic significance

Zhou

Biekemitoufu

et al. 2021

Oncol Lett

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“…Snail and Slug are zinc-finger transcription factors that serve as suppressors of E-cadherin transcription and promote EMT ( Yu et al, 2010 ; Wu et al, 2013 ; Ganesan et al, 2016 ). Furthermore, their overexpression is linked with tumor progression, invasiveness and poor survival ( Bolós et al, 2003 ; Yu et al, 2010 ; Nomura et al, 2013 ; Salehi et al, 2017 ). The inhibition of Snail, Slug or Twist through siRNA or antisense transfer leads to inhibition of tumor metastasis or growth inhibition and increased susceptibility to chemotherapeutic agents ( Vannini et al, 2007 ; Zhang et al, 2008 , 2010 ), which demonstrates the potential of manipulating EMT markers to achieve a better outcome in bladder cancer treatment.…”

Section: Discussionmentioning

confidence: 99%

The Cells and Extracellular Matrix of Human Amniotic Membrane Hinder the Growth and Invasive Potential of Bladder Urothelial Cancer Cells

Ramuta

Jerman

Tratnjek

et al. 2020

Front. Bioeng. Biotechnol.

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“…Despite the fact that many approaches have so far been applied for CRC treatment, therapy resistance as well as metastasis to regional lymph nodes and distinct organs, especially liver, still remain as major challenges . One of the most important steps in the conversion of primary tumors to metastatic form is known as epithelial‐mesenchymal transition (EMT) process . Mesenchymal markers such as vimentin , in the cells underlying this process, are overexpressed and cause progression to migratory phenotype.…”

Section: Introductionmentioning

confidence: 99%

microRNA‐193a‐5p inhibits migration of human HT‐29 colon cancer cells via suppression of metastasis pathway

Shirafkan

Shomali

Kazemi

et al. 2018

J of Cellular Biochemistry

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Purpose Altered expression of microRNAs (miRNAs) is indicated strongly in colorectal cancer (CRC). This study aims to evaluate the inhibitory role of miR‐193a‐5p on epithelial‐mesenchymal transition markers in CRC lines. The cellular effects and potential mechanisms of miR‐193a‐5p were also examined. Methods Quantitative reverse‐transcription polymerase chain reaction (RT‐PCR) was performed to determine the expression of miR‐193a‐5p in three CRC cell lines (HCT‐116, SW‐480, and HT‐29) and its impact on metastasis‐related genes ( vimentin and CXCR4) before and after mimic transfection. Of those, the cell line with the highest changes was selected for the next upcoming experiments such as wound‐healing assay, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT), and annexin‐V staining tests. Results Our results revealed that miR‐193a‐5p was significantly downregulated in three CRC cell lines and that HT‐29 displayed the most decrease ( P < 0.0001). The restoration of miR‐193a‐5p in human HT‐29 cell line inhibited cell migration. But, miR‐193a‐5p transfection did not affect cell viability and had no significant effect on apoptosis induction. Also, the quantitative RT‐PCR analysis of miR‐193a‐5p mimic transfected cells revealed a significant increase in miR‐193a‐5p messenger RNA (mRNA) expression level ( P < 0.0001) with reduction of vimentin and CXCR4 mRNA expression levels in HT‐29 cell line ( P < 0.01 and < 0.05, respectively). Conclusion Our results indicated that miR‐193a‐5p acts as a tumor suppressor miRNA and its downregulation plays an important role in metastasis via upregulation of metastasis‐related genes in CRC. Therefore, it can be considered as a potential therapeutic target for applying in CRC management in the future.

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An analysis of suppressing migratory effect on human urinary bladder cancer cell line by silencing of snail-1

Cited by 11 publications

References 16 publications

Expression of Twist, Slug and Snail in esophageal squamous cell carcinoma and their prognostic significance

Expression of Twist, Slug and Snail in esophageal squamous cell carcinoma and their prognostic significance

The Cells and Extracellular Matrix of Human Amniotic Membrane Hinder the Growth and Invasive Potential of Bladder Urothelial Cancer Cells

microRNA‐193a‐5p inhibits migration of human HT‐29 colon cancer cells via suppression of metastasis pathway

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