The Cells and Extracellular Matrix of Human Amniotic Membrane Hinder the Growth and Invasive Potential of Bladder Urothelial Cancer Cells

Ramuta, Taja Železnik; Jerman, Urška Dragin; Tratnjek, Larisa; Janev, A; Magatti, Marta; Vertua, Elsa; Signoroni, Patrizia Bonassi; Silini, Antonietta; Parolini, Ornella; Kreft, Mateja Erdani

doi:10.3389/fbioe.2020.554530

Cited by 11 publications

(8 citation statements)

References 99 publications

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“…One of the most widely analyzed hallmark of tumor cells is proliferation. Indeed, in vitro PnDs have been shown to mostly exert antiproliferative effects on tumor cells ( Gauthaman et al, 2012 ; Magatti et al, 2012 ; Liu et al, 2013 ; Hendijani et al, 2015 ; Kalamegam et al, 2019 ; Ramuta et al, 2020a ), but it has also been shown that they may induce tumor cell proliferation ( Kim et al, 2015 ; Li et al, 2015 ).…”

Section: The Effects Of Perinatal Derivatives On the Hallmarks Of Cancermentioning

confidence: 99%

“…While the precise nature of antimicrobial action of PnDs is not well understood, it is clear that more than one mechanism working simultaneously to inhibit microbe growth and endotoxin activity, contributes to this activity ( Magatti, Vertua et al, 2017 ). Antimicrobial properties of PnDs have been investigated in bacteria ( Talmi et al, 1991 ; Mao et al, 2016 ; Mao et al, 2017 ; Tehrani et al, 2017 ; Mao et al, 2018 ; Ashraf et al, 2019 ; Palanker et al, 2019 ; Šket et al, 2019 ; Ramuta et al, 2020a ), fungi ( Wang, Xie et al, 2012 ), bacteria-infected cell cultures ( Ramuta et al, 2021b ) and rat in vivo models ( Robson and Krizek 1973 ; Yadav et al, 2017 ). Antimicrobial peptides, such as α and β defensins, human cathelicidin LL37, lipocalin, elafin and secretory leukocyte protease inhibitor (SLPI), have been identified in various PnDs ( King et al, 2007a ; King et al, 2007b ; Ramuta T.Ž.…”

Section: Antimicrobial Effects Of Perinatal Derivativesmentioning

confidence: 99%

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Methods and criteria for validating the multimodal functions of perinatal derivatives when used in oncological and antimicrobial applications

Silini

Ramuta

Pires

et al. 2022

Front. Bioeng. Biotechnol.

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Perinatal derivatives or PnDs refer to tissues, cells and secretomes from perinatal, or birth-associated tissues. In the past 2 decades PnDs have been highly investigated for their multimodal mechanisms of action that have been exploited in various disease settings, including in different cancers and infections. Indeed, there is growing evidence that PnDs possess anticancer and antimicrobial activities, but an urgent issue that needs to be addressed is the reproducible evaluation of efficacy, both in vitro and in vivo. Herein we present the most commonly used functional assays for the assessment of antitumor and antimicrobial properties of PnDs, and we discuss their advantages and disadvantages in assessing the functionality. This review is part of a quadrinomial series on functional assays for the validation of PnDs spanning biological functions such as immunomodulation, anticancer and antimicrobial, wound healing, and regeneration.

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Section: The Effects Of Perinatal Derivatives On the Hallmarks Of Cancermentioning

confidence: 99%

Section: Antimicrobial Effects Of Perinatal Derivativesmentioning

confidence: 99%

Methods and criteria for validating the multimodal functions of perinatal derivatives when used in oncological and antimicrobial applications

Silini

Ramuta

Pires

et al. 2022

Front. Bioeng. Biotechnol.

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“…As the most commonly used perinatal tissue is the hAM, we assume that the poor percentage of the in vitro characterization is due to the fact that the hAM has been characterized and tested many times before. Further, identification and preparation of the hAM are unambiguous and straightforward (Jerman et al, 2014;Poženel et al, 2019;Jerman et al, 2020;Ramuta et al, 2020;Weidinger et al, 2020), whereas cells and cell-derived secretomes or sEV require precise characterization. Since the vast majority of the researchers isolated cells from the perinatal tissues by themselves, adequate characterization is mandatory to assure that a pure cell population has been isolated.…”

Section: In Vitro Characterization and Functional Testing Of Pnd Before Their Application In Preclinical Studiesmentioning

confidence: 99%

Systematic Review of the Application of Perinatal Derivatives in Animal Models on Cutaneous Wound Healing

Pichlsberger

Jerman

Obradović

et al. 2021

Front. Bioeng. Biotechnol.

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Knowledge of the beneficial effects of perinatal derivatives (PnD) in wound healing goes back to the early 1900s when the human fetal amniotic membrane served as a biological dressing to treat burns and skin ulcerations. Since the twenty-first century, isolated cells from perinatal tissues and their secretomes have gained increasing scientific interest, as they can be obtained non-invasively, have anti-inflammatory, anti-cancer, and anti-fibrotic characteristics, and are immunologically tolerated in vivo. Many studies that apply PnD in pre-clinical cutaneous wound healing models show large variations in the choice of the animal species (e.g., large animals, rodents), the choice of diabetic or non-diabetic animals, the type of injury (full-thickness wounds, burns, radiation-induced wounds, skin flaps), the source and type of PnD (placenta, umbilical cord, fetal membranes, cells, secretomes, tissue extracts), the method of administration (topical application, intradermal/subcutaneous injection, intravenous or intraperitoneal injection, subcutaneous implantation), and the type of delivery systems (e.g., hydrogels, synthetic or natural biomaterials as carriers for transplanted cells, extracts or secretomes). This review provides a comprehensive and integrative overview of the application of PnD in wound healing to assess its efficacy in preclinical animal models. We highlight the advantages and limitations of the most commonly used animal models and evaluate the impact of the type of PnD, the route of administration, and the dose of cells/secretome application in correlation with the wound healing outcome. This review is a collaborative effort from the COST SPRINT Action (CA17116), which broadly aims at approaching consensus for different aspects of PnD research, such as providing inputs for future standards for the preclinical application of PnD in wound healing.

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“…The anticancer capacity of amnion, even more, confirms the safe potential of amnion in regenerative medicine. 75 Janev et al evaluated the anticancer effect of amnion homogenate on 2D and 3D models of various human cancer and normal cells, including papillary cancer urothelial RT4 cells, muscle-invasive bladder cancer urothelial T24 cells, normal porcine urothelial NPU cells, and low-metastatic breast cancer MCF7 cells as well as human non-tumorigenic mammary gland MCF10A cells. Their result demonstrated that amnion homogenate decreased the growth, adhesion, and proliferation of cancer cells, with the most pronounced effect on T24 and RT4 cells.…”

Section: Introductionmentioning

confidence: 99%

Human amniotic membrane as a multifunctional biomaterial: recent advances and applications

et al. 2022

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The developing fetus is wrapped by a human amniotic membrane or amnion. Amnion is a promising human tissue allograft in clinical application because of its chemical composition, collagen-based, and mechanical properties of the extracellular matrix. In addition, amnion contains cells and growth factors; therefore, meets the essential parameters of tissue engineering. No donor morbidity, easy processing and storage, fewer ethical issue, anti-inflammatory, antioxidant, antibacterial, and non-immunogenic properties are other advantages of amnion usage. For these reasons, amnion can resolve some bottlenecks in the regenerative medicine issues such as tissue engineering and cell therapy. Over the last decades, biomedical applications of amnion have evolved from a simple sheet for skin or cornea repair to high-technology applications such as amnion nanocomposite, powder, or hydrogel for the regeneration of cartilage, muscle, tendon, and heart. Furthermore, amnion has anticancer as well as drug/cell delivery capacity. This review highlights various ancient and new applications of amnion in research and clinical applications, from regenerative medicine to cancer therapy, focusing on articles published during the last decade that also revealed information regarding amnion-based products. Challenges and future perspectives of the amnion in regenerative medicine are also discussed.

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The Cells and Extracellular Matrix of Human Amniotic Membrane Hinder the Growth and Invasive Potential of Bladder Urothelial Cancer Cells

Cited by 11 publications

References 99 publications

Methods and criteria for validating the multimodal functions of perinatal derivatives when used in oncological and antimicrobial applications

Methods and criteria for validating the multimodal functions of perinatal derivatives when used in oncological and antimicrobial applications

Systematic Review of the Application of Perinatal Derivatives in Animal Models on Cutaneous Wound Healing

Human amniotic membrane as a multifunctional biomaterial: recent advances and applications

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