An Analysis of Several Slide Preparation Techniques for Human Peripheral Blood Cultures

Abstract

SummarySeveral slide preparation procedures were studied in order to render a semiquantitative indication of an optimum technique or techniques, concerning the over-all cytological quality of the chromosome spreads effected by each technique. A method of scoring for karyotypically analyzable spreads is given. It is concluded that one of the techniques (technique D) be utilized for routine karyotypic studies while a combination of two of the techniques (techniques D and E) should be employed for more extensive studies.

C-Mitosis Without Colchicine in Human Leucocyte Cultures

“…All persons under study were characterized cytogenetically by utilizing sex chromatin incidence from buccal smears and karyo type studies. Short-term leucocyte cultures were processed according to a previ ously reported technique (Jenkins 1970). Morphological analysis was carried out by counting chromosomes from 30 to 40 analyzable metaphase spreads (Jenkins and Weed 1971).…”

“…Short-term leucocyte cultures were processed according to a previ ously reported technique (Jenkins 1970). Morphological analysis was carried out by counting chromosomes from 30 to 40 analyzable metaphase spreads (Jenkins and Weed 1971). Any chromosome number which repeated itself was microscopi cally analyzed to determine whether the chromosome loss or gain contributed to a separate modality.…”