The cryogenic preservation of human whole blood inoculum for short-term leucocyte chromosome microculture

Weed, Richard G.; Curcuru, Frances M.; Jenkins, Edmund C.

doi:10.1016/0011-2240(72)90047-8

Cited by 2 publications

(3 citation statements)

References 13 publications

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“…The findings of our original technique (Weed et al, 1972) showed a higher mitotic index in frozen cultures than in fresh cultures. This appears to be due to a selective effect of PHA in the freezing medium (Knight et al, 1972).…”

Section: Discussionmentioning

confidence: 88%

“…Since then, variations of a slow dilution technique have been used in leukocyte preservations (Atkins, 1962;Goodman, 1963;Ashwood-Smith, 1964;Pegg, 1965;Cavins et al, 1968;Chess et al, 1972;Oldham et al, 1976). Fast dilution techniques also have been used for the cryoprotection of leukocytes (Brody et al, 1968;Thomson and O 'Connor, 1971;Knight et al, 1972;Weed et al, 1972;Farrant et al, 1974;Strong et al, \914\Birkeland, 1976;Weiner, 1976).…”

Section: Discussionmentioning

confidence: 99%

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Slow versus Fast Postthaw Dilution for the Recovery of Lymphocytes in Whole Blood Frozen in either Dimethyl Sulfoxide or Glycerol

Weed¹,

Jenkins²

1979

Pathobiology

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It was previously shown by the authors that human whole blood was cryoprotected by dimethyl sulfoxide (DMSO) for use as inoculum in short-term lymphocyte cultures. That procedure utilized a fast postthaw dilution where the cryoprotective agent was diluted in two steps. In this paper it is shown that a five-step ‘slow’ dilution technique is much superior to the two-step ‘fast’ dilution procedure, which significantly revises our original report of 1972. This method has strikingly improved the degree of blastogenic response to phytohemagglutinin as measured by ³H-thymidine incorporation. The increase in amount of tritium incorporation was seen in cultures which were protected with either DMSO or glycerol. A similar effect was observed in unfrozen control cultures exposed to either cryoprotectant.

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Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Slow versus Fast Postthaw Dilution for the Recovery of Lymphocytes in Whole Blood Frozen in either Dimethyl Sulfoxide or Glycerol

Weed¹,

Jenkins²

1979

Pathobiology

Self Cite

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show abstract

“…3, 1981 frozen lymphocytes (Pegg, 1965) or whole blood (Weed et aL, 1972). Similar techniques have already been developed by using directly Vol.…”

Section: Discussionmentioning

confidence: 99%

A simple cryopreservation method of human blood for chromosome study

Kawasaki

Sasaki

1981

Jap J Human Genet

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A method is described of cryopreservation of human lymphocytes for cytogenetic studies. An aliquot of whole blood is pre-incubated in a PHA-added medium for at least one hour at 37~ and then stored frozen at -140~ in Eagle's MEM containing 10~ fetal bovine serum and 10~ DMSO for various periods up to one year. Thawing is made quickly in a water-bath at 37~After thorough washing with MEM, the routine PHA-added blood culture method is applied, and airdried chromosome preparations are made. The results are highly reproducible with a good number of mitotic cells sufficient for karyotyping.

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The cryogenic preservation of human whole blood inoculum for short-term leucocyte chromosome microculture

Cited by 2 publications

References 13 publications

Slow versus Fast Postthaw Dilution for the Recovery of Lymphocytes in Whole Blood Frozen in either Dimethyl Sulfoxide or Glycerol

Slow versus Fast Postthaw Dilution for the Recovery of Lymphocytes in Whole Blood Frozen in either Dimethyl Sulfoxide or Glycerol

A simple cryopreservation method of human blood for chromosome study

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