An Analysis of Distant Metastases in Oral Squamous Cell Carcinoma

Yasui, A.; Okada, Yasuo; Mataga, Izumi; Katagiri, Masataka

doi:10.2485/jhtb.19.27

Cited by 5 publications

(2 citation statements)

References 20 publications

(29 reference statements)

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“…In cancers of the oral cavity, high histopathological malignancy grade is associated with increased risks of metastases to cervical lymph nodes, lung and bone, which greatly impact the prognosis [1][2][3][4][5][6] . Moreover, early detection of these cancers is desirable so that treatment can aim at conservation of oral functions and improvement of prognosis.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical Study of Differential Expressions of Cytokeratin-13, -14, -17 and p53 in Epithelial Dysplasia and Carcinoma of the Tongue

Okada

Moride

2010

J. Hard Tissue Biology.

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In cancers of the oral cavity, high histological malignancy grade is associated with higher risk of cervical lymph nodes, lung and bone metastases, which have great impact on the prognosis. Furthermore, early detection of these cancers is desirable so that treatment can aim at conserving oral functions and improving prognosis. It is known that several percent of leukoplakia that develop in the tongue become cancerous. Leukoplakia is a clinical diagnostic term, and histopathologically most cases show hyperkeratosis with epithelial dysplasia or hyperkeratosis without epithelial dysplasia. On the other hand, difficulties in histopathological diagnosis of severe epithelial dysplasia and carcinoma in situ are often encountered. In recent years, immunohistochemical staining of cytokeratins (CK), p53 and Ki-67 for precancerous lesions and squamous cell carcinoma (SCC) of the oral cavity has been reported. However, few reports have applied a combination of several immunohistochemical stainings to diagnosis. In the present study, with the objective to achieve a higher precision in histopathological diagnosis, we conducted and compared the immunohistochemical stainings of CK13, CK17, CK14 and p53 in 10 cases each of hyperkeratosis without epithelial dysplasia, hyperkeratosis with epithelial dysplasia, carcinoma in situ, and SCC in the tongue. The following results were obtained. CK13 was positive in all 10 cases of hyperkeratosis without epithelial dysplasia, and negative in all cases of hyperkeratosis with epithelial dysplasia, carcinoma in situ, and SCC. CK17 was negative in all 10 cases of hyperkeratosis without epithelial dysplasia, and was positive in all 10 cases of hyperkeratosis with epithelial dysplasia as well as 10 cases of carcinoma in situ and 9 of 10 cases of SCC. CK14 was negative or positive only in the basal cell layer or a few rows of cells in spinous layer adjacent to the basal cell layer in cases of hyperkeratosis without epithelial dysplasia, but was positive in all the epithelial layers in all 10 cases of hyperkeratosis with epithelial dysplasia, and also positive in the tumor in all 10 cases each of carcinoma in situ and SCC. For p53, positive reaction was observed in some cells of the basal cell layer in 2 of 10 cases of hyperkeratosis without epithelial dysplasia, while high positive rates were found in hyperkeratosis with epithelial dysplasia (10 of 10 cases) and SCC (9 of 10 cases). On the other hand, only 5 of 10 cases of carcinoma in situ were positive for p53. In conclusion, although the histopathological diagnosis of epithelial dysplasia and carcinoma in situ is based on hematoxylin-eosin stained sections, immunostaining and evaluation of CK13, CK17, CK14 and p53 are useful as an adjunct to histopathological diagnosis.

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Section: Introductionmentioning

confidence: 99%

Immunohistochemical Study of Differential Expressions of Cytokeratin-13, -14, -17 and p53 in Epithelial Dysplasia and Carcinoma of the Tongue

Okada

Moride

2010

J. Hard Tissue Biology.

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“…Oral squamous cell carcinoma (OSCC) is the most common malignancy in the head and neck, accounting for over 90% of all oral malignancies 1) . Nearly 50% of OSCC patients, with the exception of those with distant metastases, have clinical or pathological lymph node metastases, and metastases to the lungs and bones have also been reported 2,3) . Several tongue-derived squamous cell carcinoma cell lines have been described, and there is an association between tongue cancer and excessive alcohol consumption, smoking history, and human papillomavirus infection [4][5][6][7][8] .…”

Section: Introductionmentioning

confidence: 99%

Establishment and Characterization of the Human Tongue Squamous Cell Carcinoma Cell Line NOKT-1

Sakuma

Takahashi

Kii

et al. 2021

J. Hard Tissue Biology.

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The squamous cell carcinoma cell line NOKT-1 was successfully established from the right tongue of a 74-yearold Japanese man. Pathological diagnosis of the original tumor was moderately differentiated squamous cell carcinoma. NOKT-1 cells were transplanted subcutaneously into nude mice and xenograft was formed. In addition, the NOKT-1-XG cell line was established from the transplanted tumor of NOKT-1 cells. NOKT-1 cells and NOKT-1-XG cells were epithelial neoplastic and pleomorphic cells, which were similar. Immunocytochemistry revealed that NOKT-1 and NOKT-1-XG cells were CK17 and human mitochondria positive. To authenticate the NOKT-1 cell line and NOKT-1-XG cell line, we examined cross-contamination with other cell lines using short tandem repeat analysis, the results of which showed that NOKT-1 and NOKT-1-XG are new cell lines. Four of the 16 loci, corresponding to 25%, were different between these two cell lines, which indicates that the NOKT-1 genome was altered by transplantation. Moreover, in AM, NOKT-1 did not have a Y chromosome, whereas NOKT-1-XG had. Despite the genetic differences, a collagen gel droplet-embedded culture drug susceptibility test demonstrated that NOKT-1 cells derived from the original tumor and the NOKT-1-XG cell line had the same sensitivity. This cell line could be very useful for the development of immunotherapy and chemotherapy regimens and research on cancer etiology.

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Establishment and characterization of human lingual squamous cell carcinoma cell lines designated Nialym derived from metastatic foci of lymph node, and Nialymx derived from transplanted tumor of Nialym cells

et al. 2015

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The squamous cell carcinoma cell lines Nialym was successfully established from metastatic foci of lymph nodes from a 48-year-old male Japanese patient with squamous cell carcinoma of the tongue. In addition, the Nialymx cell line was established from a transplanted tumor of Nialym cells in SCID mice. Nialym cells were angular, with neoplastic and pleomorphic features. Two types of Nialym cell were observed by electron microscopy; light cells and dark cells. The dark cells had a number of waved tonofilaments in the cytoplasm, while light cells showed poorly developed organelles. The population doubling times for Nialym and Nialymx cells were approximately, 46 and 42 h at the 10th passage. Nialym cells secreted 4.8 ng/ml VEGF and 5.9 ng/ml HGF, Nialymx cells also secreted 6.7 ng/ml VEGF and 4.3 ng/ml HGF at the 10th passage for 3 days of culture. Histopathological aspects of Nialym and Nialymx cell lines were similar. We believe that these cell lines are valuable tools for elucidating the mechanisms of cancer metastasis and developing immunotherapy and chemotherapy regimens.

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An Analysis of Distant Metastases in Oral Squamous Cell Carcinoma

Cited by 5 publications

References 20 publications

Immunohistochemical Study of Differential Expressions of Cytokeratin-13, -14, -17 and p53 in Epithelial Dysplasia and Carcinoma of the Tongue

Immunohistochemical Study of Differential Expressions of Cytokeratin-13, -14, -17 and p53 in Epithelial Dysplasia and Carcinoma of the Tongue

Establishment and Characterization of the Human Tongue Squamous Cell Carcinoma Cell Line NOKT-1

Establishment and characterization of human lingual squamous cell carcinoma cell lines designated Nialym derived from metastatic foci of lymph node, and Nialymx derived from transplanted tumor of Nialym cells

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