Establishment and characterization of human lingual squamous cell carcinoma cell lines designated Nialym derived from metastatic foci of lymph node, and Nialymx derived from transplanted tumor of Nialym cells

Abstract

The squamous cell carcinoma cell lines Nialym was successfully established from metastatic foci of lymph nodes from a 48-year-old male Japanese patient with squamous cell carcinoma of the tongue. In addition, the Nialymx cell line was established from a transplanted tumor of Nialym cells in SCID mice. Nialym cells were angular, with neoplastic and pleomorphic features. Two types of Nialym cell were observed by electron microscopy; light cells and dark cells. The dark cells had a number of waved tonofilaments in the cytoplasm, while light cells showed poorly developed organelles. The population doubling times for Nialym and Nialymx cells were approximately, 46 and 42 h at the 10th passage. Nialym cells secreted 4.8 ng/ml VEGF and 5.9 ng/ml HGF, Nialymx cells also secreted 6.7 ng/ml VEGF and 4.3 ng/ml HGF at the 10th passage for 3 days of culture. Histopathological aspects of Nialym and Nialymx cell lines were similar. We believe that these cell lines are valuable tools for elucidating the mechanisms of cancer metastasis and developing immunotherapy and chemotherapy regimens.

Optimal Contact Concentration of Paclitaxel in the Collagen Gel Droplet-Embedded Culture Drug Sensitivity Test for Human Oral Squamous Cell Carcinoma and Evaluation of Combination with Cetuximab

“…This study evaluated 12 human OSCC cell lines: SAT; SAS, OSC-19, OSC-20, HSC-2, HSC-3, HSC-4, SCC-4, and Nialym (tongue squamous cell carcinoma); HO-1-u-1 and KON (oral floor squamous cell carcinoma); and HO-1-N-1 (buccal squamous cell carcinoma). Nialym is a human OSCC cell line that was established in our department and has shown stable growth [16]. The other 11 cell lines were purchased from the Japanese Collection of Research Bioresources (Tsukuba, Japan), Cell Bank at the National Institute of Biomedical Innovation.…”

Optimal Cetuximab Contact Concentration Using a Collagen Gel Droplet- Embedded Culture Drug Chemosensitivity Test in Human Oral Squamous Carcinoma Cell Lines

“…This study evaluated 13 human OSCC cell lines: Ca9-22 gingival SCC, SAS lingual SCC, SAT oral SCC, HSC-2 oral SCC, HSC-3 lingual SCC, HSC-4 lingual SCC, OSC-19 lingual SCC, OSC-20 lingual SCC, HO-1-N-1 buccal SCC, HO-1-u-1 oral floor SCC, KON oral floor SCC, SCC-4 lingual SCC, and Nialym lingual SCC. The Nialym cell line is a stably growing, sub-cultured human OSCC cell line derived from a patient that underwent surgery in our department [15]. The Ca9-22 and SAS cell lines were purchased from the Health Science Research Resources Bank, and the remaining 10 cell lines were purchased from the Japanese Collection of Research Bioresources (JCRB), Cell Bank at the National Institute of Biomedical Innovation.…”

Establishment and Characterization of the Human Tongue Squamous Cell Carcinoma Cell Line NOKT-1

“…Nearly 50% of OSCC patients, with the exception of those with distant metastases, have clinical or pathological lymph node metastases, and metastases to the lungs and bones have also been reported 2,3) . Several tongue-derived squamous cell carcinoma cell lines have been described, and there is an association between tongue cancer and excessive alcohol consumption, smoking history, and human papillomavirus infection [4][5][6][7][8] . Commonly used human tongue squamous cell carcinoma cell lines include the highly invasive SAS cells and less invasive clones as well as the highly metastatic LMF3, LMF4, and LMF5 cell lines derived from human oral tongue squamous cell carcinoma.…”