An amphiphilic-ligand-modified gold nanoflower probe for enhancing the stability of lateral flow immunoassays in dried distillers grains

Ma, Tongtong; Duan, Hong; Zhang, Wenjing; Shao, Yanna; Hao, Liangwen; Chen, Xirui; Leng, Yuankui; Huang, Xiaolin; Xiong, Yonghua

doi:10.1039/c9ra06690j

Cited by 5 publications

(1 citation statement)

References 43 publications

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“…Refinement of packaging and storage conditions may improve the long-term stability of this test kit. Of note, there have been other studies which showed stability of up to 5 years with minimal impact on performance [ 38 ]. Finally, although the study was able to analyse the test line intensity using mobile phone photography for objective recording, comparisons of the test intensities in participants may be difficult, as the lighting conditions varied between different isolation rooms.…”

Section: Limitationsmentioning

confidence: 99%

Amplified parallel antigen rapid test for point-of-care salivary detection of SARS-CoV-2 with improved sensitivity

et al. 2021

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In the ongoing COVID-19 pandemic, simple, rapid, point-of-care tests not requiring trained personnel for primary care testing are essential. Saliva-based antigen rapid tests (ARTs) can fulfil this need, but these tests require overnight-fasted samples; without which independent studies have demonstrated sensitivities of only 11.7 to 23.1%. Herein, we report an Amplified Parallel ART (AP-ART) with sensitivity above 90%, even with non-fasted samples. The virus was captured multimodally, using both anti-spike protein antibodies and Angiotensin Converting Enzyme 2 (ACE2) protein. It also featured two parallel flow channels. The first contained spike protein binding gold nanoparticles which produced a visible red line upon encountering the virus. The second contained signal amplifying nanoparticles that complex with the former and amplify the signal without any linker. Compared to existing dual gold amplification techniques, a limit of detection of one order of magnitude lower was achieved (0.0064 ng·mL–1). AP-ART performance in detecting SARS-CoV-2 in saliva of COVID-19 patients was investigated using a case–control study (139 participants enrolled and 162 saliva samples tested). Unlike commercially available ARTs, the sensitivity of AP-ART was maintained even when non-fasting saliva was used. Compared to the gold standard reverse transcription-polymerase chain reaction testing on nasopharyngeal samples, non-fasting saliva tested on AP-ART showed a sensitivity of 97.0% (95% CI: 84.7–99.8); without amplification, the sensitivity was 72.7% (95% CI: 83.7–94.8). Thus, AP-ART has the potential to be developed for point-of-care testing, which may be particularly important in resource-limited settings, and for early diagnosis to initiate newly approved therapies to reduce COVID-19 severity. Graphical abstract

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Section: Limitationsmentioning

confidence: 99%

Amplified parallel antigen rapid test for point-of-care salivary detection of SARS-CoV-2 with improved sensitivity

et al. 2021

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Hydrazide-assisted directional antibody conjugation of gold nanoparticles to enhance immunochromatographic assay

Zhou

Chen

et al. 2021

Analytica Chimica Acta

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Recent advances in determination applications of emerging films based on nanomaterials

Zhang

et al. 2023

Advances in Colloid and Interface Science

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An amphiphilic-ligand-modified gold nanoflower probe for enhancing the stability of lateral flow immunoassays in dried distillers grains

Abstract: An amphiphilic ligand-capped gold nanoflower (AuNF) was proposed as a novel lateral flow immunoassay (LFA) reporter for zearalenone (ZEN) detection in distillers dried grains solubles (DDGS).

Cited by 5 publications

References 43 publications

Amplified parallel antigen rapid test for point-of-care salivary detection of SARS-CoV-2 with improved sensitivity

Amplified parallel antigen rapid test for point-of-care salivary detection of SARS-CoV-2 with improved sensitivity

Hydrazide-assisted directional antibody conjugation of gold nanoparticles to enhance immunochromatographic assay

Recent advances in determination applications of emerging films based on nanomaterials

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