Hydrazide-assisted directional antibody conjugation of gold nanoparticles to enhance immunochromatographic assay

Zhou, Shu Feng; Hu, Jing; Chen, Xirui; Duan, Hong; Shao, Yanna; Lin, Tongyu; Li, Xiangmin; Huang, Xiaolin; Xiong, Yonghua

doi:10.1016/j.aca.2021.338623

Cited by 24 publications

(17 citation statements)

References 56 publications

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“…The oriented chemical attachment of antibodies to the functionalized nanoparticles is achieved by first oxidizing the carbohydrate residue in the antibody's Fc region into aldehyde groups using periodate-based oxidation, followed by chemical condensation with nanoparticles functionalized with semicarbazide groups. 55 Thus, the oriented anti-M6PR antibodies expose their antigen binding sites ( i.e. , Fab fragments) towards the outside, making it easier for them to bind to cell surface mannose 6-phosphate receptors (M6PR).…”

Section: Resultsmentioning

confidence: 99%

Super-resolution imaging of antibody-conjugated biodegradable periodic mesoporous organosilica nanoparticles for targeted chemotherapy of prostate cancer

Das¹,

Pujals²,

Ali³

et al. 2023

Nanoscale

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Section: Resultsmentioning

confidence: 99%

Super-resolution imaging of antibody-conjugated biodegradable periodic mesoporous organosilica nanoparticles for targeted chemotherapy of prostate cancer

Das¹,

Pujals²,

Ali³

et al. 2023

Nanoscale

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“…This may be because of the antibody orientation. In the directional method, the Fab region of the antibodies is oriented outward . With antibodies stacking next to each other as they bind the NS surface, the specific orientation may create steric hindrance in which only a limited amount of Abs can physically fit next to each other on the NS surface.…”

Section: Resultsmentioning

confidence: 99%

“…In the directional method, the Fab region of the antibodies is oriented outward. 29 With antibodies stacking next to each other as they bind the NS surface, the specific orientation may create steric hindrance in which only a limited amount of Abs can physically fit next to each other on the NS surface. Using the non-directional method, the antibody orientation is random, which allows the antibodies to pack more densely by taking on more favorable positions, owing to linker flexibility and folding.…”

Section: Quantifying the Influence Of Mpeg-sh Concentration On Ns Zet...mentioning

confidence: 99%

E-Selectin Targeted Gold Nanoshells to Inhibit Breast Cancer Cell Binding to Lung Endothelial Cells

Fereshteh

Dang

Wenck

et al. 2023

ACS Appl. Nano Mater.

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Extravasation of circulating tumor cells (CTCs) from the vasculature is a key step in cancer metastasis. CTCs bind to cell adhesion molecules (CAMs) expressed by endothelial cells (ECs) for flow arrest prior to extravasation. While a number of EC-expressed CAMs have been implicated in facilitating CTC binding, this work investigated the efficacy of inhibiting cancer cell binding to human lung microvascular ECs via antibody blocking of E-selectin using antibody-functionalized gold nanoshells (NS). The antibody-functionalized gold NS were synthesized using both directional and non-directional antibody conjugation techniques with variations in synthesis parameters (linker length, amount of passivating agents, and ratio of antibodies to NS) to gain a better understanding of these properties on the resultant hydrodynamic diameter, zeta potential, and antibody loading density. We quantified the ability of E-selectin antibody-functionalized NS to bind human lung microvascular endothelial cells (HMVEC-Ls) under non-inflamed and inflamed (TNF-α) conditions to inhibit binding of triple-negative MDA-MB-231s. E-selectin-targeted NS prepared using non-directional conjugation had higher antibody loading than those prepared via directional conjugation, resulting in the conjugates having similar overall binding to HMVEC-Ls at a given antibody concentration. E-selectin-targeted NS reduced MDA-MB-231 binding to HMVEC-Ls by up to 41% as determined using an in vitro binding assay. These results provide useful insights into the characteristics of antibody-functionalized NS prepared under different conditions while also demonstrating proof of concept that these conjugates hold potential to inhibit CTC binding to ECs, a critical step in extravasation during metastasis.

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“…Recent years have witnessed an extensive increase in investigations on metallic nanoparticles (MNPs) as drug delivery agents. This is because the MNPs are highly amenable for size and shape tuneablility, − including an ability to enable surface conjugation of a wide variety of targeting moieties, such as monoclonal antibodies and peptides. − Among MNPs, gold (AuNPs) and silver nanoparticles (AgNPs) are under rigorous investigation because of their inert nature. In addition, several investigations highlight the photothermal ability of AuNPs − and AgNPs, rendering them attractive agents in thernostics. − …”

Section: Introductionmentioning

confidence: 99%

Glutathione-Capped Hollow Silver Nanoparticles: Optimization of Surface Plasmon Resonance, Photothermal Effect, and In Vitro and In Vivo Biocompatibility

Bhavsar

Joshi

Fernandes

et al. 2023

ACS Appl. Nano Mater.

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Hollow metallic nanoparticles (HNPs) serve as excellent agents for biomedical applications because they possess surface plasmon resonance (SPR) and drug-loading capacity. These HNPs offer a high surface area for drug loading and show superior antitumour activity compared to their spherical counterparts. However, the precursors used to synthesize these HNPs, particularly hollow gold nanoparticles (HAuNPs), are expensive and highly toxic. Therefore, this article proposes an alternative class of nanoparticles�hollow silver nanoparticles (HAgNPs)�that employ nontoxic and inexpensive precursors. For the first time, this article investigates several parameters involved in the synthesis to obtain uniform HAgNPs, including the effect of the stabilizer, the concentration of the reducing agent, and the reaction temperature. This article further demonstrates the photothermally induced cytotoxicity of HAgNPs in cancer cells with a 532 nm Nd:YAG 300 mW continuous-wave (CW) laser. Moreover, this article demonstrates the biocompatibility of these HAgNPs by employing in vivo and ex vivo models. Finally, we propose future studies exploring the in vivo antitumor activity of these nanoparticles to establish their role in tumor drug delivery.

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Hydrazide-assisted directional antibody conjugation of gold nanoparticles to enhance immunochromatographic assay

Cited by 24 publications

References 56 publications

Super-resolution imaging of antibody-conjugated biodegradable periodic mesoporous organosilica nanoparticles for targeted chemotherapy of prostate cancer

Super-resolution imaging of antibody-conjugated biodegradable periodic mesoporous organosilica nanoparticles for targeted chemotherapy of prostate cancer

E-Selectin Targeted Gold Nanoshells to Inhibit Breast Cancer Cell Binding to Lung Endothelial Cells

Glutathione-Capped Hollow Silver Nanoparticles: Optimization of Surface Plasmon Resonance, Photothermal Effect, and In Vitro and In Vivo Biocompatibility

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