An amperometric immunosensor with a DNA polyion complex membrane/gold nanoparticles-backbone for antibody immobilisation

Li, Na; Zhao, Hongwen; Yuan, Ruo; Peng, Kanfu; Chai, Yaqin

doi:10.1016/j.electacta.2008.08.015

Cited by 54 publications

(22 citation statements)

References 37 publications

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“…Furthermore, the Rct of anti-chlorpyrifos/AuNPs/PANI/MWCNTs/CS/GCE immunosensor (Fig. 4(d)) showed an apparent increase than the former, for that antibody acted as an inert electron layer and hindered the electron transfer [30]. At last, the Rct increased again in a similar way after BSA (Fig.…”

Section: Xrd Analysissupporting

confidence: 59%

A Novel Immunosensor Based on Au Nanoparticles and Polyaniline/Multiwall Carbon Nanotubes/Chitosan Nanocomposite Film Functionalized Interface

2013

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Abstract:A novel multilayer film based on Au nanoparticles (AuNPs) and polyaniline/carboxylated multiwall carbon nanotubes-chitosan nanocomposite (PANI/MWCNTs/CS) was exploited to fabricate a highly sensitive immunosensor for detecting chlorpyrifos. PANI-coated MWCNTs were prepared by in situ chemical polymerization and carboxylated MWCNTs played an important role in obtaining the thin and uniform coating of PANI resulting in the improved immunosensor response. AuNPs were used as a linker to immobilize chlorpyrifos antibody. The performance of the immunosensor was characterized by means of cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM), respectively. All variables involved in the preparation process and analytical performance of the immunosensor were optimized. Under optimal conditions (antibody concentration: 5 μg/mL, working buffer pH: 6.5, incubation time: 40 min, incubation temperature: 25℃), the immunosensor exhibited a wide linear range from 0.1 to 40 × 10 −6 mg/mL and from 40 × 10 −6 mg/mL to 500 × 10 −6 mg/mL, and with a detection limit of 0.06 × 10 −6 mg/mL, which provided a valuable tool for the chlorpyrifos detection in real samples.

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Section: Xrd Analysissupporting

confidence: 59%

A Novel Immunosensor Based on Au Nanoparticles and Polyaniline/Multiwall Carbon Nanotubes/Chitosan Nanocomposite Film Functionalized Interface

2013

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“…Antibodies were immobilized on gold nanoparticles and the antibodies were used for electrochemical immunosensing. 81 For the immobilization of biomolecules in polyelectrolyte complexes, it has been reported that immobilized enzymes retain higher activities than native enzymes, 82,83 and were stable. 82,84 From these reports, it is also evident that polyelectrolyte complexes are suitable materials for immobilizing biomolecules.…”

Section: ·5 Other Properties and Applicationsmentioning

confidence: 99%

Polyelectrolyte Complex Membranes for Immobilizing Biomolecules, and Their Applications to Bio-analysis

Yabuki

2011

ANAL. SCI.

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The immobilization of biomolecules is an important technique for bio-analysis, and can be applied to biosensors with both high selectivity and high sensitivity. Many researchers have developed immobilization techniques to optimize these characteristics. In the last two decades, an immobilization technique that meets the desired requirements was developed by using polyelectrolytes to form complexes, based on the electrostatic binding between polycations and polyanions. This review summarizes the techniques used for the immobilization of biomolecules by polyelectrolyte complexes; it also discusses related subjects.

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“…The water used throughout all experiments was puri¯ed through a Millipore system. Phosphate bu®er saline (PBS) was prepared by mixing the stock solutions of NaH 2 PO 4 and Na 2 HPO 4 . Human blood samples were kindly provided by the Hospital of Southeast University.…”

Section: Reagentsmentioning

confidence: 99%

“…The Chitosan-stabilized AuNPs (CHIT-AuNPs) were prepared via reduction of HAuCl 4 by NaBH 4 . Brie°y, 100 L HAuCl 4 aqueous solution with concentration of 24.3 mM was added into 10 mL (0.5%) chitosan solution (in 1% of acetic acid aqueous solution) under constant stirring for 1 h. Then, 100 L of NaBH 4 (0.1 M) was added into the resulting mixture and stirred for 1 h. The¯nal dispersion of CHIT-AuNPs was stored at 4 C for further use.…”

Section: Synthesis Of Chitosan-stabilized Aunps Compositesmentioning

confidence: 99%

“…Carcinoembryonic antigen (CEA), as a kind of membrane cellular adhesion molecule, plays a crucial role in participating in cellular adhesion to endothelium. 4,5 Moreover, CEA is commonly used as a clinical cancer marker for the diagnosis of breast cancers, colon cancers, ovarian carcinoma and cervical carcinomas. 6 A level of CEA is signi¯cantly lower in colon tissue of adults about 2.5 Â 10 3 ng/mL, but it can be increased to 5.0 ng/mL for a long-term smoker.…”

Section: Introductionmentioning

confidence: 99%

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Two Types of Immunoassay Based on Nile Blue Labeling Polydopamine Nanospheres

Liu

et al. 2017

NANO

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The sandwich-type immunoassays have been developed by using electrochemical and surfaceenhanced Raman scattering (SERS) techniques for the detection of carcinoembryonic antigen (CEA). Nile blue as a kind of Raman dye has been decorated on nanospheres with polydopamine resin (PDR) via -stacking interaction. The Nile blue displays the strong SERS signals as well as a characteristic electrochemical reduction peak at À0.33 V (versus Ag/AgCl). The implementation of the PDR nanospheres mixing with Au nanoparticles (AuNPs/PDR) exhibits a better electrical conductivity and large SERS enhancement. The immunoassays based on Nile bluelabeled AuNPs/PDR nanospheres have been fabricated by using electrochemical and SERS techniques for the detection of CEA. The proposed immunoassay shows higher sensitivity, high selectivity, lower detection limit and long-term stability. The performances of the electrochemical immunoassay are better than that of SERS immunoassay. For the electrochemical immunoassay, the linear range occurs from 1 pg/mL to 100 ng/mL (R ¼ 0:995) with a detection limit of 0.68 pg/ mL and signal-to-noise ratio of 3 in the detection of CEA. The data for the analysis of human serum samples by using the electrochemical method are acceptably consistent with those obtained from the enzyme-linked immunosorbent assay (ELISA). The proposed immunoassay exhibits a promising potential for application in clinical diagnosis.

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An amperometric immunosensor with a DNA polyion complex membrane/gold nanoparticles-backbone for antibody immobilisation

Cited by 54 publications

References 37 publications

A Novel Immunosensor Based on Au Nanoparticles and Polyaniline/Multiwall Carbon Nanotubes/Chitosan Nanocomposite Film Functionalized Interface

A Novel Immunosensor Based on Au Nanoparticles and Polyaniline/Multiwall Carbon Nanotubes/Chitosan Nanocomposite Film Functionalized Interface

Polyelectrolyte Complex Membranes for Immobilizing Biomolecules, and Their Applications to Bio-analysis

Two Types of Immunoassay Based on Nile Blue Labeling Polydopamine Nanospheres

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