An amperometric biosensor for acetylcholine determination prepared from acetylcholinesterase-choline oxidase immobilized in polypyrrole-polyvinylsulpfonate film

Development of an acetylcholinesterase–choline oxidase based biosensor for acetylcholine determination

“…By taking the electron, oxygen is reduced to H 2 O 2 . ACh determination was made by measuring the anodic current of hydrogen peroxide on electrode surface (Aynacı et al 2014). The parameters affecting the performance of the biosensor and optimum working conditions were investigated.…”

“…After electropolymerization, the mixture solution of 50.0 mL of AChE (5.0 unit/mL), 50.0 mL of ChO (5.0 unit/mL), 1.0 mg of BSA, 50.0 mL of 0.1 M phosphate buffer at pH of 8.0 and 15.0 mL of 2.5% GA was dropped on Pt/PANI-PVS film (Aynacı et al 2014). Electrode was dried at room temperature and washed with buffer solution (0.1 M phosphate buffer of pH 8.0) several times in order to remove the excess nonimmobilized enzyme and GA. Immobilized enzyme electrode was kept in a refrigerator at +4 C in phosphate buffer, when not in use.…”

“…The difference in pH values depends on the used polymer and immobilization type. Also, immobilized enzymes can show high activities under different pH conditions when compared with free enzyme (Aynacı et al 2014, Erduran et al 2013). …”

Cholinesterase-based biosensors

“…Moreover this technique could increase the attachment of the enzyme to the transducer and thus, the electron exchanges could occur more directly 10 . It is possible to use for enzyme immobilization glutaraldehyde only 44,139 , or combine it with BSA to maintain the stability of AChE 51,[140][141][142] . The main disadvantage of this method is the possibility of the activity decline caused by the distortion of the conformation of enzyme and the chemical changes of the active site during cross-linking 127 .…”

A polyaniline–zeolite nanocomposite material based acetylcholinesterase biosensor for the sensitive detection of acetylcholine and organophosphates

“…[3][4][5] Therefore, the development of analytical methods for the determination of ACh is very important. [7][8][9][10][11][12] Several reports are available in the literature for the electrochemical detection of ACh based on poly(m-(1,3)-phenylenediamine) (detection limit 0.66 mM), ceramic (detection limit 0.2 mM), copper nanoparticles (detection limit 39 mM), and poly(pyrrole)/poly(2-naphthol) (detection limit 100 nM). 2,6 Acetylcholinesterase immobilized on conducting polymers, carbon nanotubes, graphene, and carbon paste based materials have been explored for the detection of ACh.…”