An alternative method for irones quantification in iris rhizomes using headspace solid‐phase microextraction

Abstract

Irone quantification in iris rhizome can be achieved using HS-SPME-GC. This method can thus be used for the quality control of the iris rhizomes. It offers the advantage of combining extraction and analysis with an automated device and thus allows a large number of rhizome batches to be analysed and compared in a limited amount of time.

Development of a Headspace Solid‐Phase Microextraction Gas Chromatography‐Mass Spectrometry Method to Study Volatile Organic Compounds (VOCs) Emitted by Lavender Roots

“…Furthermore, between each headspace extractions, fibers were reconditioned for 5 min at 250°C. [39] 0.2 � 0.01 g of ground or intact roots was placed in a 20 mL glass vial closed with a silicone septum. Studying ground roots allows better storage of the plant material and the possibility to reproduce the experiments on the same matrix.…”

“…SPME insert (Supelco, Bellefonte, PA, USA) for both polar and non-polar analyses. [39,40] Gas Chromatography-Mass Spectrometry Analysis (GC/MS) Volatile compounds emitted from lavender roots were analyzed with a gas chromatograph model Agilent 6890 (Agilent Technologies, Palo Alto, CA, USA) coupled to a mass-selective detector (5973) and connected to a multifunction automatic sampler (Combi-Pal, CTC Analytics, Zwingen, Switzerland). Two columns were used for chemical analyses: an HP-5MS non-polar column ((5 %-phenyl)methylpolysiloxane, 30 m × 250 μm, 0.25 μm film thickness, Agilent), and an HP-INNOWax polar column (polyethylene glycol (PEG) stationary phase, 50 m × 200 μm, 0.40 μm film thickness, Agilent).…”

Screening for Changes on Iris germanica L. Rhizomes Following Inoculation with Arbuscular Mycorrhiza Using Fourier Transform Infrared Spectroscopy

“…rhizomes harvested are either decorticated and slowly dried according to traditional method up to several years or sliced and subjected to biotechnological process for a fast-induced maturation [2,5,6]. Next, "aged" orris is milled and steam distillated to obtain orris butter rich in irone and myristic acid [3,4,7,8] sold at 14640€ for 1 kg [9].…”

Efficient production of Pseudoionone with multipathway engineering in Escherichia coli

“…; Roger et al . ). Currently, pseudoionone is commercially produced via the aldol condensation of citral with acetone (Gradeff ).…”

“…Pseudoionone is a valuable acyclic intermediate for the synthesis of ionones or irones, which are extensively used as fragrances. Specifically, a-, b-ionone, and c-irone are in high demand in the fragrance and flavour industry because of their violet and woody-fruity scent (Lalko et al 2007;Roger et al 2010). Currently, pseudoionone is commercially produced via the aldol condensation of citral with acetone (Gradeff 1974).…”