An Alternative Approach to Wound Healing Field with Polypodium Vulgare

Abstract

Objective In this study, we examined the effects of Polypodium vulgare L. (Polypodiaceae) as a candidate to be used for wound healing scarred area. We investigated the antibacterial, and antioxidant activity of P. Vulgare on both in vivo, and in vitro wound healing using an excisional wound model in mice Method We used 32 Balb-c mice equally divided into four groups: Group 1 control, Group 2 vehicle, Group 3 Polypodium vulgare, and Group 4 Centella asiatica extract (CAE). All treatments were applied topically once in a day. The scar area, percentage wound closure and epithelization time were measured. PDGF, VEGF, and collagen immunohistochemical staining were used for evaluation. Results CAE and P. vulgare extract groups were observed to be more effective than the control and vehicle groups in terms of new vascular, epidermal and granulation tissue organization. PDGF, VEGF, and collagen immunohistochemical staining was stronger in the P.vulgare extract and CAE groups compared to the control and vehicle groups. In the P. vulgare and CAE groups, PDGF staining intensity was stronger than the control and vehicle groups, but VEGF and collagen staining in P. vulgare group was not different from the control group. Conclusion P. vulgare had an effect on the injured area by regenerating the epidermis and increasing vascularization. P. vulgare extract with known antioxidant, and antimicrobial activities may be helpful as a supportive treatment in wound healing.

UHPLC–PDA–Q‐TOF–MS–α‐amylase–FLD activity detection system and molecular docking

“…Based on the peak profile, the eluent was further combined and analyzed, resulting in six fractions (Fr. [1][2][3][4][5][6], and the results are shown in Figure S1. The eluent of each section was spin-dried.…”

“…Natural products have always been ideal sources for the development of clinical antidiabetic drugs because of their natural origin and low toxicity. To date, some active ingredients in plant‐derived plants have been found to inhibit specific targets, such as the main active ingredients in Crataegus pinnatifida , which inhibit cholinesterase, including flavonoids, glycosides, and organic acids 4–6 . Artemisinin in Artemisia annua inhibits the enzymes of malaria parasites 7 .…”

“…To date, some active ingredients in plant-derived plants have been found to inhibit specific targets, such as the main active ingredients in Crataegus pinnatifida, which inhibit cholinesterase, including flavonoids, glycosides, and organic acids. [4][5][6] Artemisinin in Artemisia annua inhibits the enzymes of malaria parasites. 7 Curcumin in Curcumae longae inhibits 5-lipoxygenase.…”