An alternate method for extracting DNA from environmentally challenged teeth for improved DNA analysis

Hughes‐Stamm, Sheree; Warnke, Frauke; Daal, Angela van

doi:10.1016/j.legalmed.2015.11.008

Cited by 11 publications

(4 citation statements)

References 19 publications

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“…The retrograde method that had the advantage of being minimally invasive to the tooth has been compared to grinding and spraying of the root or tooth. Although this technique created a hole for endodontic access, the amount of material retrieved was less important than with the grinding method, but the amount of amplifiable DNA per milligram of powder was significantly higher (Hughes‐Stamm, Warnke, & van Daal, 2016).…”

Section: Resultsmentioning

confidence: 99%

Ancient dental pulp: Masterpiece tissue for paleomicrobiology

Anh

Drancourt

Aboudharam

2020

Molec Gen & Gen Med

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Introduction: Dental pulp with special structure has become a good reference sample in paleomicrobiology-related blood-borne diseases, many pathogens were detected by different methods based on the diagnosis of nucleic acids and proteins. Objectives: This review aims to propose the preparation process from ancient teeth collection to organic molecule extraction of dental pulp and summary, analyze the methods that have been applied to detect septicemic pathogens through ancient dental pulps during the past 20 years following the first detection of an ancient microbe. Methods: The papers used in this review with two main objectives were obtained from PubMed and Google scholar with Results: The analysis of ancient dental pulp should have a careful preparation process with many different steps to give highly accurate results, each step complies with the rules in archaeology and paleomicrobiology. After the collection of organic molecules from dental pulp, they were investigated for pathogen identification based on the analysis of DNA and protein. Actually, DNA approach takes a principal role in diagnosis while the protein approach is more and more used. A total of seven techniques was used and ten bacteria (Yersinia pestis, Bartonella quintana, Salmonella enterica serovar Typhi, Salmonella enterica serovar Paratyphi C, Mycobacterium leprae, Mycobacterium tuberculosis, Rickettsia prowazeki, Staphylococcus aureus, Borrelia recurrentis, Bartonella henselae) and one virus (Anelloviridae) were identified. Y. pestis had the most published in quantity and all methods were investigated for this pathogen, S. aureus and B. recurrentis were identified by three different methods and others only by one. The combining methods interestingly increase the positive rate with ELISA, PCR and iPCR in Yersinia pestis diagnosis. Twenty-seven ancient genomes of Y. pestis and one ancient genome of B. recurrentis were reconstructed.Comparing to the ancient bone, ancient teeth showed more advantage in septicemic diagnosis. Beside pathogen identification, ancient pulp help to distinguish species.

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Section: Resultsmentioning

confidence: 99%

Ancient dental pulp: Masterpiece tissue for paleomicrobiology

Anh

Drancourt

Aboudharam

2020

Molec Gen & Gen Med

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“…Os métodos de extração do DNA presente no elemento dentário, pode ser através da técnica de acesso retrógrado minimamente invasivo, direto a cavidade pulpar ou o método padrão de moagem e pulverização, que se baseia no fato de que o arcabouço formado pelos tecidos de esmalte, dentina e cemento, propicia um meio estável para o DNA junto à polpa do dente, de modo que tal composto possa ser isolado e analisado, em quantidades suficientes para auxiliar na identificação humana, por fim, o reconhecimento do DNA é feito pela Reação em Cadeia da Polimerase (PCR) (KRISHAN, KANCHAN, GARG, 2015; HUGHES-STAMM, WARNKE, VAN-DAAL, 2016). No que se referem às desvantagens associadas a tais técnicas de extração do DNA dentário, evidenciam-se os altos custos operacionais e a disponibilidade de recursos tecnológicos.…”

Section: Resultsunclassified

“…No que se referem às desvantagens associadas a tais técnicas de extração do DNA dentário, evidenciam-se os altos custos operacionais e a disponibilidade de recursos tecnológicos. Aliado a isso, destaca-se a realidade de que a remoção efetiva do DNA é alcançada mediante destruição total ou parcial de todo o dente, de modo que são eliminadas as chances de identificação radiográfica, morfologia e/ou restauradora, que podem ser necessárias para identificação (HUGHES-STAMM, WARNKE, VAN-DAAL, 2016).…”

Section: Resultsunclassified

A importância da odontologia legal na identificação humana

Viana¹,

Silva²,

Lima³

et al. 2020

RSD

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“…Therefore, in an intact fresh tooth, the dental pulp is considered the best source of DNA [ 110 ]. Another preferred source for DNA extraction is dentine which is a hard dense bony tissue protected by cementum and enamel [ 111 ]. Dentine is generated from odontoblasts present in the peripheral layer in a columnar form that is extended toward the thickness of the dentin [ 112 ].…”

Section: Medical Biotechnology Techniquesmentioning

confidence: 99%

Dental Radiographic/Digital Radiography Technology along with Biological Agents in Human Identification

et al. 2022

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The heavy casualties associated with mass disasters necessitate substantial resources to be managed. The unexpectedly violent nature of such occurrences usually remains a problematic amount of victims that urgently require to be identified by a reliable and economical method. Conventional identification methods are inefficient in many cases such as plane crashes and fire accidents that have damaged the macrobiometric features such as fingerprints or faces. An appropriate recognition method for such cases should use features more resistant to destruction. Forensic dentistry provides the most appropriate available method for the successful identification of victims using careful techniques and precise data interpretation. Since bones and teeth are the most persistent parts of the demolished bodies in sudden mass disasters, scanning and radiographs are unrepeatable parts of forensic dentistry. Forensic dentistry as a scientific method of human remain identification has been considerably referred to be efficient in disasters. Forensic dentistry can be used for either “sex and age estimation,” “Medical biotechnology techniques,” or “identification with dental records,” etc. The present review is aimed at discussing the development and implementation of forensic dentistry methods for human identification. For this object, the literature from the last decade has been searched for the innovations in forensic dentistry for human identification based on the PubMed database.

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An alternate method for extracting DNA from environmentally challenged teeth for improved DNA analysis

Cited by 11 publications

References 19 publications

Ancient dental pulp: Masterpiece tissue for paleomicrobiology

Ancient dental pulp: Masterpiece tissue for paleomicrobiology

A importância da odontologia legal na identificação humana

Dental Radiographic/Digital Radiography Technology along with Biological Agents in Human Identification

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