An Algal-Derived <scp>DL</scp>-Galactan Hybrid is an Efficient Preventing Agent for <i>in vitro</i> Dengue Virus Infection

Talarico, Laura; Duarte, Maria Eugênia R.; Mello, Rosiane Guetter; Noseda, Miguel D.; Damonte, Elsa B.

doi:10.1055/s-2007-990241

Cited by 59 publications

(40 citation statements)

References 16 publications

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“…our results agree with other studies testing distinct natural products against DENV. Carbohydrate-binding agents originated from plant lectins can affect the binding of the virus to cell and subsequently inhibit viral replication (Talarico et al 2007, Alen et al 2009). Inhibitory activities by both extract and alkaloidal fraction of Uncaria tomentosa on infection rates and cytokine levels in treated monocytes-DENV infected have been reported (Reis et al 2008).…”

Section: Resultsmentioning

confidence: 99%

Virucidal activity of Colombian Lippia essential oils on dengue virus replication in vitro

Ocazionez

Meneses

Torres

et al. 2010

Mem. Inst. Oswaldo Cruz

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The inhibitory effect of Lippia alba and Lippia citriodora essential oils on dengue virus serotypes replication in vitro was investigated. The cytotoxicity (CC 50 ) was evaluated by the MTT assay and the mode of viral inhibitory effect was investigated with a plaque reduction assay. The virus was treated with the essential oil for 2 h at 37 o C before cell adsorption and experiments were conducted to evaluate inhibition of untreated-virus replication in the presence of oil. Antiviral activity was defined as the concentration of essential oil that caused 50% reduction of the virus plaque number (IC 50 ). L. alba oil resulted in less cytotoxicity than L. citriodora oil (CC 50 : 139.5 vs. 57.6 µg/mL). Virus plaque reduction for all four dengue serotypes was observed by treatment of the virus before adsorption on cell. The IC 50 values for L. alba oil were between 0. 4-32.6 µg/mL and between 1.9-33.7 µg/mL for L. citriodora oil. No viral inhibitory effect was observed by addition of the essential oil after virus adsorption. The inhibitory effect of the essential oil seems to cause direct virus inactivation before adsorption on host cell.Key words: essential oil -antiviral -virucidal activity -dengue virus -Lippia sp.Dengue is an acute illness characterised by the hemorrhagic fever caused by dengue virus (DENV) that is transmitted to humans by Aedes mosquitoes. DENV infection results in a spectrum of disease ranging from a mild febrile illness or dengue fever to a severe disease or dengue hemorrhagic fever/dengue shock syndrome. Among the human arboviral diseases, dengue is responsible for causing most illness and death. It has been estimated that 50-100 million dengue infections occur each year in tropical urban areas around the world with 20,000-25,000 deaths (Gubler 2006).DENV is a member of the Flaviviridae family and it exists as four related serotypes (DENV-1 2, 3 and 4). The virus possesses an icosahedral nucleocapsid core surrounded by a host-derived lipid membrane (envelope), in which the envelope (E) protein and membrane (M) protein are embedded. The Flavivirus enters into the host cell by receptor-mediated endocytosis into coated vesicles, a process in which the E protein plays the major role. The E protein mediates interactions within the virus and the components of the host cell membrane involved in virus adsorption and the E protein interacts with the membrane cell during fusion (Lindenbach & Rice 2007). Compounds that interact with sites on the E protein that binds to structures on the cellular membrane affect the adsorption and fusion process. That interferes There is no antiviral drug for treatment for any of the Flavivirus and an effective vaccine for human use is not yet available to prevent dengue. Consequently, the development of antiviral drugs licensed for treatment of patients remains an urgent need to prevent dengue fatalities. Compounds obtained from traditional medicinal plants and herbs species have been reported to have antiviral activity and a wide variety of active phytochemicals hav...

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Section: Resultsmentioning

confidence: 99%

Virucidal activity of Colombian Lippia essential oils on dengue virus replication in vitro

Ocazionez

Meneses

Torres

et al. 2010

Mem. Inst. Oswaldo Cruz

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“…Charged polyanions such as heparin and other sulfated polysaccharides act as HS-mimicking molecules and interfere with E protein-HS DIII target peptides Impairment of virus cell recognition [107] DIII target mAbs Impairment of virus cell recognition [25,108,109] CBAs: lectins HHA, GNA and UDA Impairment of virus cell recognition [111,112] Glycomimetic DC-SIGN ligands Impairment of virus cell recognition [113] Teicoplanin aglycon analog: LCTA-949 Impairment of virus cell recognition and probable of other entry stage [115] Sulfated galactans Inhibition of virus adsorption and penetration [46,117,118,120] Fucoidans Inhibition of virus adsorption and penetration [119,120] …”

Section: • • Inhibitors Of Virus Adsorption and Penetration Sulfated Pomentioning

confidence: 99%

“…A great variety of polysaccharides such as DL-galactan hybrids, fucoidans and carrageenans, obtained from marine algae, display anti-DENV-2 activity [20,46,[117][118][119]. It has been demonstrated that whereas DENV-2 was the most susceptible serotype to carrageenan G3d and DL-galactan hybrid C2S-3, DENV-1 was resistant to both polysaccharides.…”

Section: Carrageenans Inhibition Of Virus Adsorption and Penetrationmentioning

confidence: 99%

“…These polysulfates display anti-DENV activity in mammalian cells, but were totally inactive in mosquito cells [46]. C2S-3 and carrageenans inhibit DENV-2 adsorption and penetration, and exhibit higher effectiveness than heparin [20,118]. The antiviral activity of carrageenans is influenced by their extent of sulfation, the position of sulfate groups, the sugar composition and the number of repeated units [46].…”

Section: Carrageenans Inhibition Of Virus Adsorption and Penetrationmentioning

confidence: 99%

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Dengue Virus Entry and Trafficking: Perspectives as Antiviral Target for Prevention and Therapy

2015

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Dengue virus (DENV) is the etiological agent of the most important human viral infection transmitted by mosquitoes in the world. In spite of the serious health threat that dengue represents, at present there are no vaccine or antiviral agents available and treatment of patients consists of supportive therapy. This review will focus on the process of DENV entry into the host cell as a potential target for antiviral therapy. The recent advances in the knowledge of viral and cellular molecules and mechanisms involved in binding, internalization and trafficking of DENV into the host cell until virion uncoating are discussed, together with an overview of the strategies and compounds evaluated for development of antiviral agents targeted to DENV entry. KeywordsDengue is currently the most widespread arbovirosis in the world, with a particularly high prevalence in diverse tropical and subtropical regions of America and Asia [1]. The WHO estimates an occurrence of 50-100 million annual infections, but more recent modelling evaluations increased the number of new possible infections to 390 million per year [2]. There are four serotypes of dengue viruses (DENV), designated DENV-1 to DENV-4, that cocirculate and are transmitted to humans by the bites of the mosquitoes Aedes aegypti and Aedes albopictus. Precisely, the failure in the programs for control of the mosquito vector is one of the reasons for the explosive re-emergence and global spread of dengue in the last few decades in >100 countries, resulting in a serious public health challenge.All serotypes can produce either an inapparent infection or a wide spectrum of clinical outcomes ranging from a mild febrile illness known as dengue fever (DF) to the more severe and life-threatening forms of dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) [3]. The initial infection with one DENV serotype leads to lifelong protection against homologous reinfection, but only brief and partial protection against infection with other serotypes. In fact, the secondary infection with a heterologous serotype is considered a risk factor for developing DHF and DSS. These severe clinical manifestations have been associated to the phenomenon known as antibody-dependent enhancement (ADE) [3]. In this process, the antibodies elicited by the primary infection bind to the heterotypic virus without neutralization of viral infectivity, and these immune complexes are opsonized into Fc-receptor positive cells leading to an increase in DENV replication and pathogenesis [4,5].DENV is a member of the genus Flavivirus in the family Flaviviridae. The virion is a small spherical particle, 40-50 nm in diameter, containing a single-stranded positive sense RNA included in an inner nucleocapsid and surrounded by a lipid envelope. The virus genome codes for a single polyprotein that is cleaved into three structural proteins (the capsid protein C,

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“…Several studies have demonstrated activity of several species of algae against HIV-1 [1,2], HSV-1 [3][4][5][6], HSV-2 [4,5] and dengue type 2 strains [7]. Thus, marine algae provide a rich source for the discovery of antiviral drugs.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Zika Virus by Marine Algae

Cirne-Santos

Barros

Nogueira

et al. 2017

Preprint

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For many years marine algae has been subject of numerous researches and as a source of natural products with antiviral activity, such as terpenes, alkaloids and sulphated polysaccharides. However, the anti-Zika virus (ZIKV) potential of algae has not been studied. In this study we evaluated extracts seven species of the three major classes of seaweeds (Phaeophyceae, Chlorophyceae and Rhodophyceae) against ZIKV. All seaweeds tested are native of the Brazilian coast, except for Kappaphycus alvarezii that can be cultivated. ZIKV a mosquito-borne flavivirus, has become a public health problem. In recent years there has been an increase in the number of cases and a strong association between ZIKV outbreak and the spread of cases of Guillain-Barré Syndrome and microcephaly. All seaweed extracts tested in this work inhibits ZIKV replication in a dose-dependent manner. Caulerpa racemosa, Kappaphycus alvarezii and Osmundaria obtusiloba extracts were able to inhibit viral replication at low concentrations with EC50 values ranging from 1.38 to 1.98 µg/mL. We observed that O. obtusiloba presented a significant virucidal effect. Our results suggest that extracts of C. racemosa, K. alvarezii and O. obtusiloba presented very promising results, being excellent candidates for further studies, demonstrating that marine algae are an interesting source for the development of novel anti-ZIKV agents.

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An Algal-Derived DL-Galactan Hybrid is an Efficient Preventing Agent for in vitro Dengue Virus Infection

Cited by 59 publications

References 16 publications

Virucidal activity of Colombian Lippia essential oils on dengue virus replication in vitro

Virucidal activity of Colombian Lippia essential oils on dengue virus replication in vitro

Dengue Virus Entry and Trafficking: Perspectives as Antiviral Target for Prevention and Therapy

Inhibition of Zika Virus by Marine Algae

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