An affordable plasmid miniprep suitable for proficient microinjection in Caenorhabditis elegans

Kim, Hyun‐Min; Tian, Sisi; Wang, Shouyue

doi:10.1007/s13205-020-02346-7

Cited by 3 publications

(1 citation statement)

References 14 publications

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“…• Efficient Genome Editing in Caenorhabditis elegans with a Toolkit of Dual-Marker Selection Cassettes [12] • Precision genome editing using CRISPR-Cas9 and linear repair templates in C. elegans [42] • An Efficient Genome Editing Strategy to Generate Putative Null Mutants in Caenorhabditis elegans Using CRISPR/Cas9 [43] • CRISPR-Cas9-Guided Genome Engineering in Caenorhabditis elegans [1] • CRISPR/Cas9 Methodology for the Generation of Knockout Deletions in Caenorhabditis elegans [26] • An affordable plasmid miniprep suitable for proficient microinjection in Caenorhabditis elegans [44] • Design of Repair Templates for CRISPR-Cas9-Triggered Homologous Recombination in Caenorhabditis elegans [45] • Microinjection for precision genome editing in Caenorhabditis elegans [46] • High-efficiency CRISPR gene editing in C. elegans using Cas9 integrated into the genome [47] • Approaches for CRISPR/Cas9 Genome Editing in C. elegans [48] 6. Perspectives…”

Section: Crispr-cas Genome Editing Protocolsmentioning

confidence: 99%

A Decade of CRISPR-Cas Genome Editing in C. elegans

Kim¹,

Hong²,

Chen³

2022

Preprint

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CRISPR-Cas allows us to introduce desired genome editing, including mutations, epitopes, and deletions with unprecedented efficiency. The development of CRISPR-Cas has progressed to such an extent that it is now applicable in various fields with the help of model organisms. C. elegans is one of the pioneering animals in which numerous CRISPR-Cas strategies have been rapidly es-tablished over the past decade. Ironically, the emergence of numerous methods makes the right choice of method difficult. Choosing an appropriate selection or screening approach is the first step in planning a genome modification. This report summarizes the key features and applications of CRISPR-Cas methods using C. elegans and illustrates key strategies. Our overview of significant advances in CRISPR-Cas will help readers to understand current advances in genome editing and navigate various methods of CRISPR-Cas genome editing.

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Section: Crispr-cas Genome Editing Protocolsmentioning

confidence: 99%

A Decade of CRISPR-Cas Genome Editing in C. elegans

Kim¹,

Hong²,

Chen³

2022

Preprint

View full text Add to dashboard Cite

show abstract

Design of Repair Templates for CRISPR-Cas9-Triggered Homologous Recombination in Caenorhabditis elegans

Kim

Zhang

2021

Springer Protocols Handbooks

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A Decade of CRISPR-Cas Gnome Editing in C. elegans

Kim

Hong

Chen

2022

IJMS

Self Cite

View full text Add to dashboard Cite

CRISPR-Cas allows us to introduce desired genome editing, including mutations, epitopes, and deletions, with unprecedented efficiency. The development of CRISPR-Cas has progressed to such an extent that it is now applicable in various fields, with the help of model organisms. C. elegans is one of the pioneering animals in which numerous CRISPR-Cas strategies have been rapidly established over the past decade. Ironically, the emergence of numerous methods makes the choice of the correct method difficult. Choosing an appropriate selection or screening approach is the first step in planning a genome modification. This report summarizes the key features and applications of CRISPR-Cas methods using C. elegans, illustrating key strategies. Our overview of significant advances in CRISPR-Cas will help readers understand the current advances in genome editing and navigate various methods of CRISPR-Cas genome editing.

show abstract

An affordable plasmid miniprep suitable for proficient microinjection in Caenorhabditis elegans

Cited by 3 publications

References 14 publications

A Decade of CRISPR-Cas Genome Editing in C. elegans

A Decade of CRISPR-Cas Genome Editing in C. elegans

Design of Repair Templates for CRISPR-Cas9-Triggered Homologous Recombination in Caenorhabditis elegans

A Decade of CRISPR-Cas Gnome Editing in C. elegans

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