A Decade of CRISPR-Cas Gnome Editing in C. elegans

Abstract

CRISPR-Cas allows us to introduce desired genome editing, including mutations, epitopes, and deletions, with unprecedented efficiency. The development of CRISPR-Cas has progressed to such an extent that it is now applicable in various fields, with the help of model organisms. C. elegans is one of the pioneering animals in which numerous CRISPR-Cas strategies have been rapidly established over the past decade. Ironically, the emergence of numerous methods makes the choice of the correct method difficult. Choosing an appropriate selection or screening approach is the first step in planning a genome modification. This report summarizes the key features and applications of CRISPR-Cas methods using C. elegans, illustrating key strategies. Our overview of significant advances in CRISPR-Cas will help readers understand the current advances in genome editing and navigate various methods of CRISPR-Cas genome editing.

Advantages and Challenges of CRISPR-Cas9 Applications in Animal Modeling: A Concise Review CRISPR-Cas9 Applications in Animal Modeling

“…Various genetic models, such as zebrafish, Caenorhabditis elegans, and Drosophila, also benefits from CRISPR's technology and genome modification. It is worthwhile to mention that different studies have reported hereditary germinal modification, the introduction of high efficiency of specific mutations, and transgenic, tissue-specific editing in flies (33)(34)(35)(36)(37)(38), fish (39)(40)(41)(42)(43)(44)(45), and worms (46)(47)(48)(49)(50)(51)(52)(53)(54)(55)(56).…”

CRISPR-Cas9 genome editing inSteinernemaentomopathogenic nematodes

“…The CRISPR-Cas9 system has been successfully adopted in various nematode species, including the classical model C. elegans, the necromenic nematode Pristionchus pacificus, the mammalian parasite Strongyloides stercoralis, and other non-model species, such as Aunema , Oscheius, and Panagrolaimus (Adams et al, 2019; Castelletto & Hallem, 2021; Dockendorff et al, 2022; Hellekes et al, 2023; Hiraga et al, 2021; H.-M. Kim et al, 2022; Lok, 2019). In these successful cases, either plasmid encoded Cas9 and guide RNAs or Cas9 RNP were delivered through microinjection into the gonad of a hermaphrodite or a female nematode.…”