An advanced molecularly imprinted electrochemical sensor for the highly sensitive and selective detection and determination of Human IgG

Liang, Axin; Hou, Baoting; Tang, Cong; Sun, Duanping; Luo, E. Aiqin

doi:10.1016/j.bioelechem.2020.107671

Cited by 34 publications

(17 citation statements)

References 77 publications

(78 reference statements)

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“…In particular, the linear range of the PPE sensor extends to 2 orders of magnitude lower concentrations than the other sensors, which is a record sensitivity at low concentrations for an EIS-based IgG sensor. These research results indicate that PPE-based EIS sensors have highly reproducible and ultrasensitive characteristics. − …”

Section: Resultsmentioning

confidence: 70%

Toward a Practical Impedimetric Biosensor: A Micro-Gap Parallel Plate Electrode Structure That Suppresses Unexpected Device-to-Device Variations

Honda

Kusaka

et al. 2022

ACS Omega

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We propose a rational electrode design concept for affinity biosensors based on electrochemical impedance spectroscopy to substantially suppress unexpected device-to-device variations. On the basis that the uniformity of the current distribution affects the variation, a novel micro-gap parallel plate electrode (PPE) was developed, where two planar electrodes with edges covered with a SiO 2 layer were placed face to face. The structure provides a uniform current distribution over the planar electrode surface and maximizes the contribution of the planar electrode surface to sensing. For a comparative study, we also fabricated a micro-structured interdigitated electrode (IDE) that has been widely adopted for high-sensitivity measurement, although its current is highly concentrated on the electrode edge corner. Protein G (PrG) molecules were immobilized on both electrodes to prepare an immunoglobulin G (IgG) biosensor on which the specific binding of PrG–IgG can occur. We demonstrated that the IgG sensor with the PPE has small device-to-device variations, in strong contrast to the sensor with the IDE having large device-to-device variations. The results indicate that the current distribution on the electrode surface is important to fabricating electrochemical impedance spectroscopy biosensors with small device-to-device variations. Furthermore, it was found that the PPE allows ultrasensitive detection, that is, the sensor exhibited a linear range from 1 × 10 –13 to 1 × 10 –7 mol/L with a detection limit of 1 × 10 –14 mol/L, which is a record sensitivity at low concentrations for EIS-based IgG sensors.

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Section: Resultsmentioning

confidence: 70%

Toward a Practical Impedimetric Biosensor: A Micro-Gap Parallel Plate Electrode Structure That Suppresses Unexpected Device-to-Device Variations

Honda

Kusaka

et al. 2022

ACS Omega

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“…Electrochemical biosensing has an enormous impact on the biosensing of IgG, and a broad range of studies have been conducted to attain the unique, selective, sensitive, and economical characteristics of a biosensor. Liang et al, has reported a molecular imprinted electrochemical biosensor for selective and extremely sensitive detection of IgG [ 57 ]. The GCE (glassy carbon electrode) was modified using a MoS2@N-GQDs-IL (molybdenum disulfide @nitrogen-doped graphene quantum dots with ionic liquid) nanocomposite.…”

Section: Biosensors For the Detection Of Iggmentioning

confidence: 99%

Emerging Methods in Biosensing of Immunoglobin G—A Review

Azam

Bukhari

Liaqat

et al. 2023

Sensors

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Human antibodies are produced due to the activation of immune system components upon exposure to an external agent or antigen. Human antibody G, or immunoglobin G (IgG), accounts for 75% of total serum antibody content. IgG controls several infections by eradicating disease-causing pathogens from the body through complementary interactions with toxins. Additionally, IgG is an important diagnostic tool for certain pathological conditions, such as autoimmune hepatitis, hepatitis B virus (HBV), chickenpox and MMR (measles, mumps, and rubella), and coronavirus-induced disease 19 (COVID-19). As an important biomarker, IgG has sparked interest in conducting research to produce robust, sensitive, selective, and economical biosensors for its detection. To date, researchers have used different strategies and explored various materials from macro- to nanoscale to be used in IgG biosensing. In this review, emerging biosensors for IgG detection have been reviewed along with their detection limits, especially electrochemical biosensors that, when coupled with nanomaterials, can help to achieve the characteristics of a reliable IgG biosensor. Furthermore, this review can assist scientists in developing strategies for future research not only for IgG biosensors but also for the development of other biosensing systems for diverse targets.

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“…Significantly, with relatively low conductivity, it needs to accommodate additional nanomaterials to be operational [ 74 ]. Axin Liang et al [ 75 ] modified GCE with highly conductive MoS 2 @N-GQDs-IL to magnify electrochemical signals for human immunoglobulin G (IgG). MoS 2 was functionalized with nitrogen-doped GQDs (N-GQDs).…”

Section: Applications Of Nanomaterials In Mip Sensorsmentioning

confidence: 99%

Recent Advances of Nanomaterials-Based Molecularly Imprinted Electrochemical Sensors

Dong

Zhang

et al. 2022

Nanomaterials

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Molecularly imprinted polymer (MIP) is illustrated as an analogue of a natural biological antibody-antigen system. MIP is an appropriate substrate for electrochemical sensors owing to its binding sites, which match the functional groups and spatial structure of the target analytes. However, the irregular shapes and slow electron transfer rate of MIP limit the sensitivity and conductivity of electrochemical sensors. Nanomaterials, famous for their prominent electron transfer capacity and specific surface area, are increasingly employed in modifications of MIP sensors. Staying ahead of traditional electrochemical sensors, nanomaterials-based MIP sensors represent excellent sensing and recognition capability. This review intends to illustrate their advances over the past five years. Current limitations and development prospects are also discussed.

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An advanced molecularly imprinted electrochemical sensor for the highly sensitive and selective detection and determination of Human IgG

Abstract: Graphical abstract Schematic 1 Electrochemical determination of human IgG using CuFe 2 O 4 nanospheres molecularly imprinted polymers modified with MoS 2 @N-GQDs-IL.

Cited by 34 publications

References 77 publications

Toward a Practical Impedimetric Biosensor: A Micro-Gap Parallel Plate Electrode Structure That Suppresses Unexpected Device-to-Device Variations

Toward a Practical Impedimetric Biosensor: A Micro-Gap Parallel Plate Electrode Structure That Suppresses Unexpected Device-to-Device Variations

Emerging Methods in Biosensing of Immunoglobin G—A Review

Recent Advances of Nanomaterials-Based Molecularly Imprinted Electrochemical Sensors

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