An advanced centrifugal microsystem toward high-throughput multiplex colloidal nanocrystal synthesis

Park, Byung Hyun; Kim, Dahin; Jung, Jae-Hwan; Oh, Seung Jun; Choi, Goro; Lee, DohChang; Seo, Tae-Seok

doi:10.1016/j.snb.2014.12.067

Cited by 17 publications

(6 citation statements)

References 27 publications

(31 reference statements)

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“…Defining liquid volumes for optimal sample-reagent ratios; blood plasma separation; 6569-73 realtime PCR (aliquoting); nanoparticle synthesis 67,68…”

Section: Resultsmentioning

confidence: 99%

“…65 Volume splitting was implemented by Andersson and Ekstrand, who created a zigzag hydrophilic channel design, geometrically defining a series of volumes before sending each fraction to perform its respective test. 66 This feature can be used to vary the concentrations of the reagents to automatically synthesize different nanoparticles, as shown by Park et al in an application that synthesizes nanoparticles of various colors, 67 and in another application that synthesizes anisotropic metallic nanoparticles. 68 Optimization of this technique with other functional units in a system can yield high-throughput synthesis of a great variety of nanoparticles for the monitoring of biological or chemical assays.…”

Section: Volume Definitionmentioning

confidence: 99%

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Lab-on-a-CD: A Fully Integrated Molecular Diagnostic System

et al. 2016

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The field of centrifugal microfluidics has experienced tremendous growth during the past 15 years, especially in applications such as lab-on-a-disc (LoD) diagnostics. The strength of LoD systems lies in its potential for development into fully integrated sample-to-answer analysis systems. This review highlights the technologies necessary to develop the next generation of these systems. In addition to outlining valving and other fluid-handling operations, we discuss the recent advances and future outlook in four categories of LoD processes: reagent storage, sample preparation, nucleic acid amplification, and analyte detection strategies.

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“…Defining liquid volumes for optimal sample-reagent ratios; blood plasma separation; 6569-73 realtime PCR (aliquoting); nanoparticle synthesis 67,68…”

Section: Resultsmentioning

confidence: 99%

Section: Volume Definitionmentioning

confidence: 99%

Lab-on-a-CD: A Fully Integrated Molecular Diagnostic System

et al. 2016

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“…1C). 26,27 The zigzag-shaped microchannels have the dimensions of 800 μm (width) × 500 μm (depth) × 7445 μm (length) and were filled with 25.6 μL of reagents by capillary force. The dispensed reagents were loaded into each reactor in the bottom layer through the holes and the connecting microchannels at 3000 rpm.…”

Section: Design and Fabrication Of The Centrifugal Direct-rpa Microde...mentioning

confidence: 99%

A centrifugal direct recombinase polymerase amplification (direct-RPA) microdevice for multiplex and real-time identification of food poisoning bacteria

et al. 2016

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In this study, we developed a centrifugal direct recombinase polymerase amplification (direct-RPA) microdevice for multiplex and real-time identification of food poisoning bacteria contaminated milk samples. The microdevice was designed to contain identical triplicate functional units and each unit has four reaction chambers, thereby making it possible to perform twelve direct-RPA reactions simultaneously. The integrated microdevice consisted of two layers: RPA reagents were injected in the top layer, while spiked milk samples with food poisoning bacteria were loaded into sample reservoirs in the bottom layer. For multiplex bacterial detection, the target gene-specific primers and probes were dried in each reaction chamber. The introduced samples and reagents could be equally aliquoted and dispensed into each reaction chamber by centrifugal force, and then the multiplex direct-RPA reaction was executed. The target genes of bacteria spiked in milk could be amplified at 39 °C without a DNA extraction step by using the direct-RPA cocktails, which were a combination of a direct PCR buffer and RPA enzymes. As the target gene amplification proceeded, the increased fluorescence signals coming from the reaction chambers were recorded in real-time at an interval of 2 min. The entire process, including the sample distribution, the direct-RPA reaction, and the real-time analysis, was accomplished with a custom-made portable genetic analyzer and a miniaturized optical detector. Monoplex, duplex, and triplex food poisoning bacteria (Salmonella enterica, Escherichia coli O157:H7, and Vibrio parahaemolyticus) detection was successfully performed with a detection sensitivity of 4 cells per 3.2 μL of milk samples within 30 min. By implementing the direct-PRA on the miniaturized centrifugal microsystem, the on-site food poisoning bacteria analysis would be feasible with high speed, sensitivity, and multiplicity.

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“…A wide range of platforms have been developed, using a variety of actuation principles as well as different detection methods Chin et al, 2012;W. Jung et al, 2015;Park et al, 2015;. Among the multitude of available actuation principles, centrifugal microfluidics, i.e.…”

Section: Introductionmentioning

confidence: 99%