Detection methods for centrifugal microfluidic platforms

Burger, Robert; Amato, Letizia; Boisen, Anja

doi:10.1016/j.bios.2015.06.075

Cited by 71 publications

(58 citation statements)

References 85 publications

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“…Acoustic‐based detection methods also have high sensitivity and can be relatively easily integrated into the microchip, but the working frequency range is different for different samples. To overcome these disadvantages, applications of multiple detection methods have been reported in recent years . The composite method can achieve high precision and multiparameter detection.…”

Section: Detection In Microfluidic Flow Cytometrymentioning

confidence: 99%

New advances in microfluidic flow cytometry

Gong

Fan

et al. 2018

Electrophoresis

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In recent years, researchers are paying the increasing attention to the development of portable microfluidic diagnostic devices including microfluidic flow cytometry for the point‐of‐care testing. Microfluidic flow cytometry, where microfluidics and flow cytometry work together to realize novel functionalities on the microchip, provides a powerful tool for measuring the multiple characteristics of biological samples. The development of a portable, low‐cost, and compact flow cytometer can benefit the health care in underserved areas such as Africa or Asia. In this article, we review recent advancements of microfluidics including sample pumping, focusing and sorting, novel detection approaches, and data analysis in the field of flow cytometry. The challenge of microfluidic flow cytometry is also examined briefly.

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Section: Detection In Microfluidic Flow Cytometrymentioning

confidence: 99%

New advances in microfluidic flow cytometry

Gong

Fan

et al. 2018

Electrophoresis

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“…However, while the LoaD technique has simplified basic operations, solutions to other arising needs, such as the integration of (active) operations, or the readout of a bioassay result, has proven more challenging to achieve when the platform is under continuous spinning motion. Without the possibility of a direct connection of sensors and/or actuators to a stationary power supply, stroboscopic observation and excitation has remained the most widely means of detection and actuation in LoaD systems (Burger et al, 2016). These include absorbance (colorimetric), fluorescence and chemiluminescence detection methods for many biological and chemical assays, as well as laser scanning microscope systems, imaging based detectors.…”

Section: Introductionmentioning

confidence: 99%

“…These include absorbance (colorimetric), fluorescence and chemiluminescence detection methods for many biological and chemical assays, as well as laser scanning microscope systems, imaging based detectors. Even resonance detection via vibration and deflection sensing techniques for mechanical (cantilever-based) sensors have been reported (Burger et al, 2016;Kong et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

The eLoaD platform endows centrifugal microfluidics with on-disc power and communication

Delgado

Korvink

Mager

2018

Biosensors and Bioelectronics

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In this paper we present a comprehensive description of the design, fabrication and operation of an electrified Lab-on-a-Disc (eLoaD) system. The smart platform is developed to extend conventional Lab-on-a-Disc applications with an electronic interface, providing additional flow control and sensing capabilities to centrifugal microfluidics platforms. Wireless power is transferred from a Qi-compliant transmitter to the eLoaD platform during rotation. An Arduino-based microcontroller, a Bluetooth communication module, and an on-board SD-card are integrated into the platform. This generalises the applicability of the eLoaD and its modules for performing a wide range of laboratory unit operations, procedures, or diagnostic assays, all controlled wirelessly during spinning. The lightweight platform is fully reusable and modular in design and construction. An interchangeable and non-disposable application disc is fitted with the necessary sensors and/or actuators for a specific assay or experiment to be performed. A particular advantage is the ability to continuously monitor and interact with LoaD experiments, overcoming the limitations of stroboscopy. We demonstrate the applicability of the platform for two sensing experiments involving optical, electrochemical, and temperature detection, and one actuation experiment involving controlled heating/cooling, and flow control with active valves. The complete electronic designs and example programming codes are extensively documented in the supplementary material for easy adaptation.

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“…These systems can be further sub-divided into 'Bioanalytical Screening CDs' and 'Lab-on-a-Disc (LoaD)'. Bioanalytical screening CDs focus on adapting optical disc drive (ODD) technology (e.g., Compact Disc, DVD) for biological detection [9]. This technology takes advantage of the high-density data storage capability as well as the favorable pricing for its main constituents, i.e., the optical pickup unit, the spindle drive, and the optical disc substrates [10][11][12][13][14].The LoaD [15][16][17][18][19][20] is typically used to automate the standard laboratory steps conducted in a biology lab to enable a fully automated sample-to-answer system.…”

mentioning

confidence: 99%

Siphon-Induced Droplet Break-Off for Enhanced Mixing on a Centrifugal Platform

Burger¹,

Kinahan

Cayron

et al. 2019

Inventions

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We present a powerful and compact batch-mode mixing and dilution technique for centrifugal microfluidic platforms. Siphon structures are designed to discretize continuous flows into a sequence of droplets of volumes as low as 100 nL. Using a passive, self-regulating 4-step mechanism, discrete volumes of two fluids are alternatingly issued into a common intermediate chamber. At its base, a capillary valve acts as a fluidic shift register; a single droplet is held in place while two or more droplets merge and pass through the capillary stop. These merged droplets are advectively mixed as they pass through the capillary valve and into the receiving chamber. Mixing is demonstrated for various combinations of liquids such as aqueous solutions as well as saline solutions and human plasma. The mixing quality is assessed on a quantitative scale by using a colorimetric method based on the mixing of potassium thiocyanate and iron(III) chloride, and in the case of human plasma using a spectroscopic method. For instance, volumes of 5 µL have been mixed in less than 20 s. Single-step dilutions up to 1:5 of plasma in a standard phosphate buffer solution are also demonstrated. This work describes the preliminary development of the mixing method which has since been integrated into a commercially available microfluidic cartridge.Inventions 2020, 5, 1 2 of 14 microfluidic systems constitute a subset which uses the rotation of the chip, which is typically of a similar geometry as a Compact Disc™ or DVD™, to enable most pumping and valving operations. These systems can be further sub-divided into 'Bioanalytical Screening CDs' and 'Lab-on-a-Disc (LoaD)'. Bioanalytical screening CDs focus on adapting optical disc drive (ODD) technology (e.g., Compact Disc, DVD) for biological detection [9]. This technology takes advantage of the high-density data storage capability as well as the favorable pricing for its main constituents, i.e., the optical pickup unit, the spindle drive, and the optical disc substrates [10][11][12][13][14].The LoaD [15][16][17][18][19][20] is typically used to automate the standard laboratory steps conducted in a biology lab to enable a fully automated sample-to-answer system. The LoaD is particularly applicable for deployment in the field (point-of-use) or at the hospital bed-side (point-of-care) as the chips can be loaded at atmospheric pressure (via pipette or syrette) without issues regarding sealing or priming. The use of centrifugation for pumping also reduces the cost and complexity of support instrumentation as just low-cost spindle motors are required rather than specialized micropumps. Similarly, inherent centrifugation of samples [21][22][23] applied to sample preparation is a particular strength.The technological precursor of these systems are the centrifugal analyzers which became popular in the 1970s and 1980s [24] for automating a very limited number of simple assay steps, on rather macroscopic sample volumes, and readout on a rotor-based instrument. Currently the main areas of application are immuno...

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Detection methods for centrifugal microfluidic platforms

Cited by 71 publications

References 85 publications

New advances in microfluidic flow cytometry

New advances in microfluidic flow cytometry

The eLoaD platform endows centrifugal microfluidics with on-disc power and communication

Siphon-Induced Droplet Break-Off for Enhanced Mixing on a Centrifugal Platform

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