An adhesion molecule-based colloidal gold immunochromatography assay strip for rapidly and specifically detecting chicken antibodies against Mycoplasma gallisepticum

Zhang, Jinliang; Guo, Yan; Hu, Sishun; Bi, Dingren; Wang, Xiliang; Xiao, Yufei; Gao, Xiaocong; Li, Zili; Liu, Mei; Peng, Fuhu; Xu, Qiang

doi:10.1016/j.rvsc.2010.06.020

Cited by 4 publications

(2 citation statements)

References 14 publications

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“…The accurate and rapid detection of M. gallisepticum is the first and important step for controlling the infection. Serological assays namely plate agglutination and ELISA are routine screening tests and confirmation can be done either by culture or molecular techniques (Ramadass et al, 2006;Zhang et al, 2011). Despite being laborious, expensive and time-consuming cultural isolation is still gold standard for detecting avian mycoplasmas.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Phylogenetic Analysis of Avian Mycoplasmas from Poultry Affected with Respiratory Infections in India

Singh

Tomar

Mahajan

et al. 2020

IJAR

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Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are the two most pathogenic avian mycoplasmas. In the present study, examination of 92 pooled tissue samples from broiler chicken of 92 different poultry flocks of Haryana (India) exhibiting respiratory infections resulted in isolation of 13 (14%) Mollicutes. Based on biochemical reactions, growth inhibition test, PCR and/or sequencing, 8 (8.6%) isolates could be characterized as MG, 1 (1.08%) as MS, 3 (3.24%) as M. gallinarum and 1 (1.08%) as Acholeplasma laidlawii. The phylogenetic analysis using Intergenic spacer region (IGSR) of these MG isolates revealed that they clustered with USA strain whereas the vaccine strains were in different clade. Single locus sequence typing (SLST) revealed considerable nucleotides variation between 8 MG isolates and vaccine strains. Conclusively, Sequencing of IGSR region of MG can be used as a valuable epidemiological investigation tool for the differentiation of wild-type MG strains from vaccine strains.

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Section: Introductionmentioning

confidence: 99%

Isolation and Phylogenetic Analysis of Avian Mycoplasmas from Poultry Affected with Respiratory Infections in India

Singh

Tomar

Mahajan

et al. 2020

IJAR

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“…To date, the accurate and rapid diagnosis for MG is essential to prevent and control the infection by this pathogen. Many detection methods were developed and researched recently, including serology, culture, and molecular identification (Garcia et al, 2005;Ghorashi et al, 2010;Kahya et al 2010;Zhang et al 2011). Although isolation of mycoplasmas is the gold standard way for determining MG, time-consuming for this method limits its wide application within poultry production (Levisohn and Kleven, 2000).…”

Section: Introductionmentioning

confidence: 99%

Development of a novel real-time polymerase chain reaction (PCR) assay by amplification of double target genes for quantitative detection of Mycoplasma gallisepticum

Hu¹,

Li²

2014

Afr. J. Biotechnol.

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Mycoplasma gallisepticum (MG) is the most pathogenic and economically significant pathogen in poultry worldwide. Detection of MG is the first and important step for controlling its transmission. A novel real-time polymerase chain reaction (PCR) assay targeting two conservative genes was developed and evaluated carefully in the study. The 16S rRNA and fMG-2 gene fragments were cloned into PCR 2.1 vectors, and recombinant plasmids (r16S21 and rFmg21) were evaluated by PCR, double digestion and nucleotide sequencing. SYBR green real-time PCR was developed using two purified plasmids as templates, and the amplification conditions and reaction systems of real-time PCR were optimized based on specificity, sensitivity and repeatability. A pair of standard curves were assembled for the realtime PCR by detecting two target genes 16S rRNA and fMG-2. The real-time PCR is highly specific to the target genes, with a detection limit of 9 copies/μl. The result of reproducibility shows that the real-time PCR remained consistent. The result of clinical samples demonstrated that the detection rate of the assay was significantly higher than that of the conventional PCR. The double target genes real-time PCR was highly specific, sensitive, and reproducible and could be used on clinical samples from commercial chickens.

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Mycoplasmosis

Chin

2013

Diseases of Poultry

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An adhesion molecule-based colloidal gold immunochromatography assay strip for rapidly and specifically detecting chicken antibodies against Mycoplasma gallisepticum

Cited by 4 publications

References 14 publications

Isolation and Phylogenetic Analysis of Avian Mycoplasmas from Poultry Affected with Respiratory Infections in India

Isolation and Phylogenetic Analysis of Avian Mycoplasmas from Poultry Affected with Respiratory Infections in India

Development of a novel real-time polymerase chain reaction (PCR) assay by amplification of double target genes for quantitative detection of Mycoplasma gallisepticum

Mycoplasmosis

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