An Acylase from Shewanella Putrefaciens Presents a Vibrio Parahaemolyticus Acylhomoserine Lactone-Degrading Activity and Exhibits Temperature-, Ph- and Metal-Dependences

Abstract

Shewanella putrefaciens supernatant was found to increase the virulence factors of Vibrio parahaemolyticus by efficiently degrading its acylhomoserine lactone (AHL). To further reveal the regulation mechanism and its key degrading enzyme, a potential AHL-degrading enzyme acylase (Aac) from S. putrefaciens was cloned, and the influences of temperature, pH, protein modifiers, and metals on Aac were tested. Aac was significantly influenced by temperature and pH, and exhibited the highest AHL-degrading activity at temperatures of 37 °C and pH of 8. Mg2+ and Fe2+ can further increase the AHL-degrading activity. 10 mM EDTA inhibited its activity possibly by chelating the co-factors (metals) required for Aac activity. Tryptophan and arginine were identified as key components for Aac activity that are critical to its AHL-degrading activity. This study provides useful information on Aac and for V. parahaemolyticus control.

Elekti̇f Total Abdomi̇nal Hi̇sterektomi̇ Ve Bi̇lateral Ooferektomi̇ Yapilan Hastalarda İntraoperati̇f Farkli Dozlarda Uygulanan Li̇dokai̇n İnfüzyonun Postoperati̇f Ağri Kontrolüne Ve Analjezi̇ Tüketi̇mi̇ne Etki̇leri̇ni̇n Gözlenmesi̇

“…Jinekolojik cerrahi tipleri için ise kısıtlı veriler bulunmaktadır (6,7). Aynı zamanda yapılan çalışmalar sadece sistemik lidokain infüzyonu ile IV opioid arasında karşılaştırma yapılmıştır (8,9). Bu nedenle biz total abdominal histerektomi ve bilateral ooferektomi (TAH-BSO) hastalarında farklı dozlardaki lidokain infüzyonunun parasetamol tüketimiyle birlikte postoperatif analjezik etkisi ve postoperatif bulantı üzerine olan etkisini araştırmayı amaçladık.…”