An acridine-linked oligodeoxynucleotide targeted to the common 5' end of trypanosome mRNAs kills cultured parasites

UV-induced early-domain binding factor as the limiting component of simian virus 40 DNA amplification in rodent cells.

“…For instance, probes complementary to the splice junction of herpes simplex virus (HSV) immediate-early pre-mRNAs specifically inhibited HSV protein synthesis, replication, and viral growth in Vero cells (37). Antisense oligonucleotides to the common miniexon structure of trypanosome mRNAs killed cultured trypanosomes (44). In those experiments, the oligonucleotides were stabilized by covalently attached acridine or methylphosphonate, and rather high concentrations were required.…”

Inhibition of Plasmodium falciparum malaria using antisense oligodeoxynucleotides.

“…In vitro, AS ODNs have been shown to inhibit replication of both Trypanosoma brucei (6) and Leishmania amazonensis (7). In vivo, AS ODNs have been used to cure ducks of hepatitis B…”

Blockage of AM V reverse transcriptase by antisense oligodeoxynucleotides

“…Acridinelinked oligonucleotides elicit RNase-H activity when bound to the complementary RNA sequence [20]. Moreover, it has been shown that such modified antisense oligomers can be used with intact cells, and prevent the development of the influenza virus [21] and of African trypanosomes in culture [14]. Our results indicate that they might also be of interest in the case of retroviruses.…”

“…The mini-exon sequence present at the 5' end of every mRNA of the parasite was previously chosen as a target for antisense oligonucleotide [12][13][14][15]. We transcribed the T. brucei med-RNA from a primer located immediately downstream of the mini-exon sequence (Fig.…”