An Acetylcholine Electrochemical Biosensor Based on Bi-Enzyme Functionalized Nanofiber Composites

Abstract

This work offered an electrochemical biosensor for acetylcholine (ACh) detection using the bi-enzyme functionalized nanofiber composites-modified glassy carbon electrode (GE). The nanofiber composites (AuNPs@GCS) prepared by electrospinning and electrodeposition techniques, were systematically characterized from micromorphology, active groups, actual areas, and impedance, and were used to simultaneously decorate the acetylcholinesterase (AChE) and choline oxidase (ChOx). Bi-enzyme functionalized AuNPs@GCS (AChE-ChOx/AuNPs@GCS) electrode demonstrated superior loading capacity and stability, and when used for ACh analysis, the detection limit was 0.067 nM, outperforming previous sensors for linear range and LOD. After systematic methodological evaluation and feasibility verification, this biosensor had good stability, specificity, and reproducibility. In human serum samples, the results detected by the biosensor were compared with results tested by Enzyme-linked immunosorbent assay. T test was performed for statistical comparisons and p value more than 0.05 was considered no statistical significance. It indicated that this biosensor had a good application prospect in the ACh actual sample detection. This work also provided an efficient and accurate method for quantitative detection of neurotransmitters in complex biological samples.

Electrochemical Acetylcholinesterase Sensors for Anti-Alzheimer’s Disease Drug Determination

“…On the other hand, acetylcholine does not produce an electrochemically active product that could be detected by amperometry. The introduction of a second enzyme, ChO, solves the problem (Figure 7) [65,90]. The use of the thioanalog of a natural substrate, acetylcholine, complic comparison of the inhibition effects with the toxic consequences of poisoning beca nature of the substrate influences the kinetics of reversible inhibition.…”

“…On the othe acetylcholine does not produce an electrochemically active product that could be d by amperometry. The introduction of a second enzyme, ChO, solves the problem 7) [65,90]. The design of a bi-enzyme sensor is complicated by the difference in the o pH of the AChE and ChO activity and in the specific enzyme activity of the prep used in the immobilization step.…”

Review—Electrochemical Sensors for Acetylcholine Detection

“…2D. 107 Recently, Jing et al 2023 108 developed an advanced biosensor for detecting ACh. They used nanofiber composites (AuNPs@GCS) on a glassy carbon electrode, fabricated through electrospinning and electrodeposition.…”