Amylostereum laevigatum associated with the Japanese horntail, Urocerus japonicus

Abstract

The fungus associated with the Japanese horntail, Urocerus japonicus, in Kochi, Kagawa and Ehime Prefectures was studied. Cultures isolated from the mycangia of 113 adult females of the horntail showed the same cultural characteristics. Four of basidiocarps found on felled logs of Cryptomeriajaponica were identified as Amylostereum laevigatum based on morphological characteristics. This was the first record of A. laevigatum from Japan. The cultures isolated from the basidiocarps had the same cultural characteristics as those from the mycangia of U.japonicus. One mycangial isolate produced basidiocarps on artificially inoculated stem segments of Cr. japonica after a 6-too incubation and was identified as A. laevigatum. One isolate from the basidiocarps of A. laevigatum and one from the mycangium of U. japonicus were artificially inoculated into five trees each of Chamaecyparis obtusa and Cr. japonica. The wood of all inoculated trees showed discoloration, with no difference in shape and pattern of discoloration between the two isolates. The inoculated fungi were reisolated from the areas of discoloration in the inoculated trees.

Host specificity and diversity of Amylostereum associated with Japanese siricids

“…The collection data from this and other studies in Japan and Europe (Tabata and Abe, 1997;Vasiliauskas et al, 1998;Tabata and Abe, 1999;Vasiliauskas, 1999;Vasiliauskas and Stenlid, 1999;Tabata et al, 2000) appears to suggest that the tree host may be a non-trivial driver of the fungal and wasp species association. For example, A. chailletii always appears to be associated with tree hosts in the Pinaceae, but outside the genus Pinus (i.e.…”

Amylostereum laevigatum associated with a horntail, Urocerus antennatus

“…Inoculation of stem segments and trees was performed following the procedures described by Tabata and Abe (1997). One mycangial isolate (FD-166, Table 1 ) was cultured on PDA at 25~ in darkness for 3 wk and inoculated into four stem segments of Cr.…”

“…The horntails that were trapped on the sticky paper were collected twice a month between May and August. The symbiotic fungus was isolated from the mycangia following the method of Tabata and Abe (1997). The fungus taken from the mycangia was mounted in lactophenol and observed under a light microscope.…”

An Olfactometer Testing the Olfactory Response of the Japanese Horntail Urocerus japonicus to Volatiles.

“…Urocerus japonicus; Hymenoptera: Siri cidae 1989 1992 1997 Knight andHeikkenen, 1980 1992 Amylostereum laevigatum Tabata and Abe, 1997 Haack and Slansky, 1987Hanks, 19991997Simpson 1976 Sirex noctilio EAG Electron Antenno Gram;…”