Amylose content controls the V-type structural formation and in vitro digestibility of maize starch-resveratrol complexes and their effect on human gut microbiota

Chlorogenic Acid/Linoleic Acid-Fortified Wheat-Resistant Starch Ameliorates High-Fat Diet-Induced Gut Barrier Damage by Modulating Gut Metabolism

“…Solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR) analysis was performed on a Bruker AVANCE III HD 400 spectrometer equipped with a 4 mm MAS solid Magnet 600 probe (Bruker, Germany). 20 The spectra of the C1 peak included single helix (102−103 ppm) and double-helix (99−101 ppm) conformations. By subtracting a scaled amorphous starch spectrum until zero intensity was attained at 84 ppm, the total spectra were deconvoluted and used to calculate the contents of the helical structures, which included the amorphous structure, the single-helix structure, and the double-helix structure.…”

“…Identical to the in vitro digestion experiment, the simulated digestion formula was used. At scheduled time intervals (5,10,20,60, and 120 min), 0.5 mL of sample was sampled from the release medium and mixed with hexane, and then the extracted LA was analyzed spectrophotometrically at 211 nm. 26 CA was measured by the Folin-Ciocalteu method according to the previous study.…”

“…Solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR) analysis was performed on a Bruker AVANCE III HD 400 spectrometer equipped with a 4 mm MAS solid Magnet 600 probe (Bruker, Germany) . The spectra of the C1 peak included single helix (102–103 ppm) and double-helix (99–101 ppm) conformations.…”