Amylase hyperproduction by deregulated mutants of the thermophilic fungus Thermomyces lanuginosus

Rubinder, K; Chadha, B. S.; Singh, Narpinder; Saini, Harvinder Singh; Singh, Surendra

doi:10.1038/sj.jim.7000270

Cited by 23 publications

(11 citation statements)

References 27 publications

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“…(2) where, X 1 -pH, X 2 -temperature in 0 C and X 3 -inoculum size in %. The larger the magnitudes of F-value, the smaller the pvalue, the more significant value is the corresponding coefficient (Akhnazarova and Kafarov, 1982;Rubinder et al, 2002). The results of the second order response surface model fitting in the form of analysis of variance (ANOVA) are in Table. 6.…”

Section: Resultsmentioning

confidence: 99%

Optimization of amylase production from Bacillus sp. using statistics<br>based experimental design

Zambare¹

2011

Emir. J. Food Agric

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Production of amylase under submerged fermentation Bacillus sp. was investigated using wheat bran, soybean meal and CaCO 3 (WSC) medium. Response surface methodology (RSM) was used to evaluate the effect of the main variables, i.e., pH (11.35), temperature (35.16°C) and inoculum size (2.95%) on amylase production by applying a full factorial central composite design (CCD). The mutual interaction between these variables resulted into 4.64 fold increase in amylase activity as compared to the non-optimized environmental factors in the basal medium.

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Section: Resultsmentioning

confidence: 99%

Optimization of amylase production from Bacillus sp. using statistics<br>based experimental design

Zambare¹

2011

Emir. J. Food Agric

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“…Phutela et al (2005) reported an optimum temperature of 60°C for thermophilus A. fumigatus pectinase. Other thermophilic fungi have been reported to optimally produce enzymes at 50°C (Rubinder et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

A comparative study of pectinolytic enzyme production by Bacillus species

Torimiro¹,

Okonji²

2013

Afr. J. Biotechnol.

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The economic and ecological function of pectinase enzymes in industries is gaining much attention with the need of highly productive strains of microorganisms to reduce production cost. The present investigation is a comparative evaluation of Bacillus sp. pectinases. The three isolates isolated from agro-waste, Bacillus stearothermophilus, Bacillus cereus and Bacillus subtilis, showed very high pectinase activities with pectin as the substrate. The three isolates also showed varying degree of preference for banana peels and wheat bran but their activity towards orange peel was very low compared to other substrate. The effect of temperature on the pectinase of the three isolates showed that B. stearothermophilus had optimum temperature at 60°C while B. cereus and B. subtilis both showed optimum activity at 50°C. On the effect of pH, B. stearothermophilus, B. cereus and B. subtilis showed optimum pectinase activities at pH 7.5, 8.0 and 9.0, respectively. Metal ions enhanced the activity of pectinase produced by the three isolates. The study exhibited that B. stearothermophilus gave the most pectinase activity, optimum temperature and a moderate alkaline pH, possibly making it a better source for industrial purpose.

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“…Using semi-quantitative plate assay approach, a thermophilic fungal strain TF3 (Aspergillus fumigatus MTCC 4163) isolated from decomposing orange peel waste, was selected as the best producer of pectinase and PG. The plate assay approach has been previously employed in our lab for isolating hyper amylase producing mutants/fusants of thermophilic fungus Thermomyces lanuginosus (22,23).…”

Section: Discussionmentioning

confidence: 99%

“…The potency index of each isolate was calculated as the ratio of zone diameter to colony diameter. The cultures showing high potency index were further screened by semiquantitative plate assay method (22,23). The cultures were individually plated on YPSS medium (9) containing pectin in place of starch and incubated at 50ºC.…”

Section: Screening Of Thermophilic Fungal Isolates For Pectinolytic Amentioning

confidence: 99%

Pectinase and polygalacturonase production by a thermophilic Aspergillus fumigatus isolated from decomposting orange peels

Phutela¹,

Dhuna²,

Sandhu³

et al. 2005

Braz. J. Microbiol.

110

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A thermophilic fungal strain producing both pectinase and polygalacturonase was isolated after primary screening of 120 different isolates. The fungus was identified as Aspergillus fumigatus Fres. MTCC 4163. Using solid-state cultivation, the optimum levels of variables for pectinase and polygalacturonase (PG) production were determined. Maximal levels of enzyme activities were achieved upon growing the culture in a medium containing wheat bran, sucrose, yeast extract and (NH4)2SO4 after 2-3 days of incubation at a temperature of 50ºC. Highest enzyme activities of 1116 Ug -1 for pectinase and 1270 Ug -1 for polygalacturonase were obtained at pH 4.0 and 5.0, respectively.

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Amylase hyperproduction by deregulated mutants of the thermophilic fungus Thermomyces lanuginosus

Cited by 23 publications

References 27 publications

Optimization of amylase production from Bacillus sp. using statistics<br>based experimental design

Optimization of amylase production from Bacillus sp. using statistics<br>based experimental design

A comparative study of pectinolytic enzyme production by Bacillus species

Pectinase and polygalacturonase production by a thermophilic Aspergillus fumigatus isolated from decomposting orange peels

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