Amylase Activity of Starch Degrading Bacteria Isolated from Soil

Nimisha, Patel; Moksha, Shah; Gangawane, Ajit K.

doi:10.20546/ijcmas.2019.804.071

Cited by 59 publications

(68 citation statements)

References 3 publications

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“…The results of the research showed data in accordance with Mishra and Behera (2008), who reported that bacterial isolates exhibiting amylase activity have high amylase activity at 40-45°C, especially Bacillus species. The results also concurred with a study by Gebreselema (2015), which found the optimum temperature for amylase activity to be 40°C in Bacillus and 37°C in Streptomyces.…”

Section: Effect Of Temperature On Amylase Ac Vitysupporting

confidence: 86%

Amylolytic ability of bacteria isolated from termite (Coptotermes sp.) gut

Mulyani

Hamid

Janatunaim

et al. 2018

Indones. J. Biotechnol.

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BSR 2, BSR 3, BSR 8, and BSR 9, different bacteria isolated from the termite gut, have been shown to possess cellulolytic activities, but their amylolytic ability has heretofore been unknown. This study attempted to fill in this knowledge gap. The formation of a clear zone using the iodine test showed that the bacteria were able to produce and secrete amylase. Based on the results, the best cultivation times for strains BSR 2, BSR 3, BSR 8, and BSR 9 were 6, 3, 2, and 2 d, respectively, yielding amylase activities of 2.59 ± 0.13 U/mg, 2.00 ± 0.08 U/mg, 1.67 ± 0.10 U/mg, and 1.55 ± 0.12 U/mg, respectively. BSR 2 had the highest amylase activity compared with the other bacterial isolates. The optimum ph for bacterial amylase activity of BSR 2 was 7.0, and the optimum temperature was 40°C. The molecular characterization of isolates BSR 2, BSR 3, BSR 8, and BSR 9 was based on 16S rRNA gene sequences. Isolates BSR 8 and BSR 9 were thus identified as Brevibacillus parabrevis and Brevibacillus sp. With similarities amounting to 92.48% and 95.91%, while the BSR 3 isolate was identified as Pseudomonas alcaligenes with a similarity of 94.29%, and the BSR 2 isolate could not be identified yet.

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Section: Effect Of Temperature On Amylase Ac Vitysupporting

confidence: 86%

Amylolytic ability of bacteria isolated from termite (Coptotermes sp.) gut

Mulyani

Hamid

Janatunaim

et al. 2018

Indones. J. Biotechnol.

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“…Soil samples of various origins were collected from starchy fields of Lahore, Pakistan. Alpha amylase producing bacteria were screened from the local soil habitat by plating the samples on Luria Bertani agar containing starch as a source of substrate [17][18][19]. One gram of the soil sample was added in 10 ml of sterilized distilled water, followed by shaking and incubation 15 min at 80 o C. 50µl from above sample was then plated on the agar medium supplemented with starch.…”

Section: Materials and Methods Sample Collection Isolation And Screementioning

confidence: 99%

Comparative study of alpha amylase producing mutated and unmutated Ba-cillus strains by taking cost effective measures

Ramzan

Chaudhry²,

Bashir³

et al. 2019

PAB

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Bacillus species is considered as most appropriate bacterial specie for extracellular production of alpha amylase at commercial level. The alpha amylase is in high demand in a lot of food, beverage, and textile industries. In recent years, researchers have made many attempts to scale up the production of alpha amylase by using various strategies. The current study deals with the isolation, identification of Bacillus species and evaluation of their alpha amylase activity by random mutagenesis. Amylase producing bacteria were isolated from soil sample and further categorized via 16S rRNA technique. Random mutagenesis technique (MNNG and UV radiation) was employed to enhance the production of an enzyme. Mutants were then assessed for alpha amylase production via solid state fermentation using different agro-industrial waste, i.e. potato peels, apple peels, banana peels, wheat bran and rice husk. Different parameters such as incubation temperature, incubation period and pH were optimized to obtain maximum yield. Optimum amylase production (207.84U/ml) was obtained after 48 hours of incubation at 37°C by using MNNG mutant and potato peels. This study reveals that Bacillus cereus has the potential of alpha amylase production. It further suggests that secretion of alpha amylase can further be maximized by improving the strain genetically and optimizing the fermentation conditions.

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“…Some species are included in the clade B is known as a bacteria with high amylase activity, namely B. subtilis (Espino et al 1997;Halami 2008), B. amylolyquefaciens (Apun et al 2000) and B. licheniformis (Mishra & Behera 2008). PPK5 isolate which is a bacterial isolate with the highest amylase activity between the two other amylolytic bacterial isolates (Table 1), joined with clade B and it is closely related to B. subtilis.…”

Section: Identification Of the Amylolytic Bacteria Producing Of Phb Bmentioning

confidence: 99%

Polyphasic Identification of Amylolytic Bacteria Producing Bioplastic Poly-β-hydroxybutyrate (PHB)

Yanti¹,

Sembiring²,

Margino³

et al. 2019

JSM

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The final goal of this study is to make a modern systematic-based inventory of amylolytic bacterial isolates producing of bioplastic Poly-β-hydroxybutyrate (PHB) from sago starch substrate. The identity of three local bacterial isolates was examined in this study, using a polyphasic approach. A data set based on phenotypic characteristics, namely morphological, physiological, biochemical and chemical character, namely whole cells protein profiles using SDS-PAGE method, together with phylogenetic studies based on 16S rRNA sequences was used to identified by polyphasic approach. Phenotypic characteristics of 3 local bacterial isolates and 4 reference strains to members of genus Bacillus was analyzed by numerical analysis using MVSP 3,1 program to determine the value of similarity. Based on the preliminary characterization of the profile matching method showed that the three isolates of bacteria producing PHB namely PSA10, PPK5 and PPK6 are members of the genus Bacillus. The results of numerical analysis based on phenotypic characteristic and chemical character of the three bacterial isolates producing PHB with reference strains showed that the PSA10 isolate bacterial identical with Bacillus megaterium, PPK5 isolate identical with Bacillus subtilis and PPK6 isolate identical with Bacillus cereus, and these results also support by the molecular phylogenetic analysis. Therefore, the polyphasic taxonomy is an effective approach to uncover the identity of the novel bacterial isolates. ABSTRAKMatlamat akhir kajian ini adalah untuk membuat inventori berasaskan sistematik moden daripada pencilan bakteria amilolisis yang menghasilkan bioplastik Poli-β-hidroksibutirat (PHB) daripada substrat kanji sagu. Identiti tiga pencilan bakteria tempatan telah diperiksa dalam kajian ini, menggunakan pendekatan polifasa. Satu set data berdasarkan ciriciri fenotip, iaitu sifat morfologi, fisiologi, biokimia dan kimia, iaitu profil protein sel keseluruhan menggunakan kaedah SDS-PAGE, bersama-sama dengan kajian filogenetik berdasarkan urutan 16S rRNA digunakan untuk mengenal pasti dengan pendekatan polifasa. Ciri-ciri fenotip 3 pencilan bakteria tempatan dan 4 rujukan kepada ahli genus Bacillus dianalisis dengan analisis berangka menggunakan MVSP 3,1 program untuk menentukan nilai keserupaan. Berdasarkan pencirian permulaan kaedah pencocokan profil menunjukkan bahawa tiga pencilan bakteria yang menghasilkan PHB iaitu PSA10, PPK5 dan PPK6 adalah ahli genus Bacillus. Hasil analisis berangka berdasarkan pencirian fenotip dan sifat kimia ketiga pencilan bakteria yang menghasilkan PHB dengan strain rujukan menunjukkan bahawa PSA10 seiras dengan Bacillus megaterium, PPK5 seiras dengan Bacillus subtilis dan PPK6 seiras dengan Bacillus cereus, dan keputusan ini juga disokong oleh analisis filogenetik molekul. Oleh itu, taksonomi polifasa adalah pendekatan yang berkesan untuk mendedahkan identiti novel pencilan bakteria.

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Amylase Activity of Starch Degrading Bacteria Isolated from Soil

Cited by 59 publications

References 3 publications

Amylolytic ability of bacteria isolated from termite (Coptotermes sp.) gut

Amylolytic ability of bacteria isolated from termite (Coptotermes sp.) gut

Comparative study of alpha amylase producing mutated and unmutated Ba-cillus strains by taking cost effective measures

Polyphasic Identification of Amylolytic Bacteria Producing Bioplastic Poly-β-hydroxybutyrate (PHB)

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