Amycolatopsis suaedae sp. nov., an endophytic actinomycete isolated from Suaeda maritima roots

Chantavorakit, Tanatorn; Suksaard, Paweena; Matsumoto, Atsuko; Duangmal, Kannika

doi:10.1099/ijsem.0.003546

Cited by 11 publications

(9 citation statements)

References 31 publications

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“… Strains: (1) YIM 96748 T ; (2) A. cihanbeyliensis BNT52 T ( Tatar et al, 2013 ); (3) A. jiangsuensis K LBMP 1262 T ( Xing et al, 2013 ); (4) A. suaedae 8-3EHSu T ( Chantavorakit et al, 2019 ). +, positive; -, negative.…”

Section: Resultsmentioning

confidence: 99%

“… Strains: (1) YIM 96748 T ; (2) A. cihanbeyliensis BNT52 T ( Tatar et al, 2013 ); (3) A. jiangsuensis K LBMP 1262 T ( Xing et al, 2013 ); and (4) A. suaedae 8-3EHSu T ( Chantavorakit et al, 2019 ).. –, not present or is a minor component. DPG, diphosphatidylglycerol; L, unidentified polar lipids; PI, phosphatidylinositol; PE, phosphatidylethanolamine; PL, unidentified phospholipids; AL, unidentified amino lipid; PME, phosphatidylmonomethylethanolamine; GL, unknown glycolipid; APL, unknown aminophospholipid; OH-PE, hydroxyphosphatidylethanolamine; NR, not reported.…”

Section: Resultsmentioning

confidence: 99%

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Amycolatopsis aidingensis sp. nov., a Halotolerant Actinobacterium, Produces New Secondary Metabolites

Wang

Ren

et al. 2021

Front. Microbiol.

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A novel actinobacterium, strain YIM 96748T, was isolated from a saline soil sample collected from the south bank of Aiding Lake in Xinjiang Province, Northwest China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 96748T is closely related to Amycolatopsis cihanbeyliensis BNT52T (98.9%) and Amycolatopsis jiangsuensis KLBMP 1262T (97.2%). The DNA–DNA relatedness between strain YIM 96748T and its closest type strain A. cihanbeyliensis BNT52T was 59.6%. The average nucleotide identity between strain YIM 96748T and its neighbor strain was 88.97%. Based on the genotypic and phenotypic characteristics, it is concluded that strain YIM 96748T represents a novel species of the genus Amycolatopsis, whose name was proposed as Amycolatopsis aidingensis sp. nov. The type strain is YIM 96748T. To investigate the biosynthetic potential of producing secondary metabolites, the complete genome of YIM 96748T was sequenced and analyzed. The complete genome sequence of YIM 96748T consists of a 7,657,695-bp circular chromosome, comprising 7,162 predicted genes with a DNA G + C content of 70.21 mol%. Fifty-one putative biosynthetic gene clusters of secondary metabolites were found, including the antibacterial/antitumor agent TLN-05220, the antibacterial agent nocardicin A, the antifungal agent nystatin A1, and the osmolyte ectoine. The investigation of the secondary metabolites of A. aidingensis YIM96748T led to the discovery of two new phenylpropyl acetate enantiomers, amycoletates A (1) and B (2), and five known compounds: 4-hydroxy phenethyl acetate (3), 2-p-acetoxyphenylethanol (4), (S)-ethyl indole-3-lactate (5), (R)-ethyl indole-3-lactate (6), and p-hydroxybenzoic acid (7). One of the gene clusters 14, 36, and 43, which contain a single module of polyketide synthase, might be responsible for the biosynthesis of compounds 1 and 2 from compound 7 as a precursor. Further studies, including the one strain many compounds approach (OSMAC) and genetic modification, are needed to explore novel compounds from this talented halophilic Amycolatopsis strain.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Amycolatopsis aidingensis sp. nov., a Halotolerant Actinobacterium, Produces New Secondary Metabolites

Wang

Ren

et al. 2021

Front. Microbiol.

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“…They have been reported from soil [4], ocean sediment [5], algae [6] and salt mines [7]. Recently, they have been isolated from endophytic environments, including Amycolatopsis suaedae from Suaeda maritima roots [8], Amycolatopsis stemonae from the stem of Stemona sp. [9] and Amycolatopsis anabasis from the roots of Anabasis elatior [10].…”

Section: Full-textmentioning

confidence: 99%

Amycolatopsis dendrobii sp. nov., an endophytic actinomycete isolated from Dendrobium heterocarpum Lindl.

Tedsree

Tanasupawat

Sritularak

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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Three novel actinomycete strains, designated as DR6-1T, DR6-2 and DR6-4, isolated from the roots of Dendrobium heterocarpum Lindl in Thailand were studied using a polyphasic taxonomic approach. The strains grew at 20–37 °C, at pH 5–10 and with 5 % (w/v) NaCl. They contained meso-diaminopimelic acid in the cell-wall peptidoglycan and MK-9(H4) was a major menaquinone. Arabinose and galactose were the major sugars in the cell wall. The predominant cellular fatty acids were iso-C16 : 0 and iso-C15 : 0. The detected polar lipids were diphosphatidylglycerol, hydroxyphosphatidylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylglycerol. Strains DR6-1T, DR6-2 and DR6-4 shared 99.9–100 % 16S rRNA gene sequence similarity and were closely related to Amycolatopsis echigonensis JCM 21831T (98.7-98.8%). The approximate genome size of strain DR6-1T was 9.6 Mb with a G+C content of 69.6 mol%. The ANIb and dDDH values between genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T were 90.55, 92.25, 92.60%, and 47.20, 52.10 and 52.50%, respectively. Based on the phenotypic, chemotaxonomic and genotypic characteristics, it has been concluded that strains DR6-1T, DR6-2 and DR6-4 represent a novel species of the genus Amycolatopsis for which the name Amycolatopsis dendrobii sp. nov. is proposed. The type strain is DR6-1T (=JCM 33742T=KCTC 49546T=TISTR 2840T).

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“…The genus was first proposed by Lechevalier et al [1] and the genus description was emended by Lee [2] in 2009, and then by Tang et al [3] in 2010, further recently by Nouioui et al [4] in 2018. Members of the genus Amycolatopsis have been isolated from a wide variety of environmental sources, such as soil [5], salt mine [6], salt lake [3], cave [7], ocean sediment [8], catacomb [9], human sources [10] and plant tissues [11]. At the time of writing this article, the genus Amycolatopsis encompasses 76 species with validly published names and four subspecies (LPSN, http://www.…”

Section: Introductionmentioning

confidence: 99%

“…html). Among them, five species have been reported to belong endophytic strains: Amycolatopsis samaneae [12] isolated from the roots of Samanea saman, Amycolatopsis suaedae [11] isolated from the roots of Suaeda maritima, Amycolatopsis stemonae [13] isolated from the stems of Stemona sp., Amycolatopsis jiangsuensis [14] isolated from the stems of Dendranthema indicum, and Amycolatopsis endophytica [15] isolated from the seeds of Jatropha curcas. In the course of our research into the diversity of the culturable endophytic bacteria in the halophyte plant Anabasis elatior, strain EGI 650086 T was isolated and purified from this target plant.…”

Section: Introductionmentioning

confidence: 99%

Amycolatopsis anabasis sp. nov., a novel endophytic actinobacterium isolated from roots of Anabasis elatior

Wang

Gao

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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A novel endophytic actinobacterium, designated strain EGI 650086T, was isolated from the roots of Anabasis elatior (C.A.Mey.) Schischk. collected in Xinjiang, north-west China. The taxonomic position of the strain was investigated using a polyphasic taxonomic approach. Growth occurred at 15–40 °C, pH 6.0–8.0 and in the presence of 0–6 % NaCl (w/v). Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %). Sequence similarities with type strains of other species of the genus Amycolatopsis were less than 97.0 %. The average nucleotide identity and DNA–DNA hybridization values between strain EGI 650086T and the reference strains were 78.1–79.8 % and 22.1–23.0 %, respectively. The genome of strain EGI 650086T was 10.9 Mb, with a DNA G+C content of 70.1 mol%. The diagnostic diamino acid in the peptidoglycan was meso-diaminopimelic acid. The major whole-cell sugars contained arabinose, galactose, glucose and ribose. The predominant menaquinones were MK-9 (H4) and MK-9 (H2). Major fatty acids were iso-C16 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B). The polar lipid profile of strain EGI 650086T included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, two unknown phospholipids, an unknown glycolipid and an unknown lipid. Polyphasic taxonomic characteristics indicated that strain EGI 650086T represents a novel species of the genus Amycolatopsis , for which the name Amycolatopsis anabasis sp. nov. is proposed. The type strain is EGI 650086T (=KCTC 49044T=CGMCC 4.7188T).

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Amycolatopsis suaedae sp. nov., an endophytic actinomycete isolated from Suaeda maritima roots

Cited by 11 publications

References 31 publications

Amycolatopsis aidingensis sp. nov., a Halotolerant Actinobacterium, Produces New Secondary Metabolites

Amycolatopsis aidingensis sp. nov., a Halotolerant Actinobacterium, Produces New Secondary Metabolites

Amycolatopsis dendrobii sp. nov., an endophytic actinomycete isolated from Dendrobium heterocarpum Lindl.

Amycolatopsis anabasis sp. nov., a novel endophytic actinobacterium isolated from roots of Anabasis elatior

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