Amycolamycins A and B, Two Enediyne-Derived Compounds from a Locust-Associated Actinomycete

Ying, Shi; Xiao, Yong; Zhang, Bo; Shao, Fen Li; Zhong, Guo; Zhang, Juan Juan; Jiao, Rui Hua; Sun, Yang; Xu, Qiang; Tan, Ren Xiang; Ge, Hui

doi:10.1021/acs.orglett.7b03113

Cited by 31 publications

(18 citation statements)

References 26 publications

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“…With ow cytometry and Western blot analysis, it was demonstrated that amycolamycin A suppressed the proliferation of M231 cells through inducing apoptosis through the activation of caspase-3. 10 Li et al isolated two new abscisic acid-type sesquiterpenes (11)(12) and one new ansamycin (13), together with four known ansamycins from Amycolatopsis alba DSM 44262. However, all of these examples showed no evident bacterial inhibition.…”

Section: Introductionmentioning

confidence: 99%

The secondary metabolites of rare actinomycetes: chemistry and bioactivity

et al. 2019

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Section: Introductionmentioning

confidence: 99%

The secondary metabolites of rare actinomycetes: chemistry and bioactivity

et al. 2019

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“…The genome of strain ncl2 T , unlike that of N. vaccinii NBRC 15922 T , contains presumptive NP-BGCs associated with the synthesis of amycolamycin A and B (2% gene similarity), type I polyketides that are cytotoxic for breast cancer cell lines [ 79 ], pepticinnamin E (6% gene similarity) which has the potential to treat cancer and malaria [ 80 ] and echosides A, B, C, D and E (11% gene similarity) and stambomycin A to D (36% gene similarity),which are antitumor antibiotics [ 81 ]. Strain ncl2 T also contains bioclusters predicted to encode for the antitubercular polyketides atratumycin (7% gene similarity) and capreomycin A and B (6% gene similarity), which are produced by ‘ Streptomyces aratus’ and ‘ Streptomyces capreolus’, respectively [ 32 , 82 ].…”

Section: Resultsmentioning

confidence: 99%

Genome insights into the pharmaceutical and plant growth promoting features of the novel species Nocardia alni sp. nov

Nouioui

Baek

et al. 2022

BMC Genomics

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Background Recent studies highlighted the biosynthetic potential of nocardiae to produce diverse novel natural products comparable to that of Streptomyces, thereby making them an attractive source of new drug leads. Many of the 119 Nocardia validly named species were isolated from natural habitats but little is known about the diversity and the potential of the endophytic nocardiae of root nodule of actinorhizal plants. Results The taxonomic status of an actinobacterium strain, designated ncl2T, was established in a genome-based polyphasic study. The strain was Gram-stain-positive, produced substrate and aerial hyphae that fragmented into coccoid and rod-like elements and showed chemotaxonomic properties that were also typical of the genus Nocardia. It formed a distinct branch in the Nocardia 16S rRNA gene tree and was most closely related to the type strains of Nocardia nova (98.6%), Nocardia jiangxiensis (98.4%), Nocardia miyuensis (97.8%) and Nocardia vaccinii (97.7%). A comparison of the draft genome sequence generated for the isolate with the whole genome sequences of its closest phylogenetic neighbours showed that it was most closely related to the N. jiangxiensis, N. miyuensis and N. vaccinii strains, a result underpinned by average nucleotide identity and digital DNA-DNA hybridization data. Corresponding taxogenomic data, including those from a pan-genome sequence analysis showed that strain ncl2T was most closely related to N. vaccinii DSM 43285T. A combination of genomic, genotypic and phenotypic data distinguished these strains from one another. Consequently, it is proposed that strain ncl2T (= DSM 110931T = CECT 30122T) represents a new species within the genus Nocardia, namely Nocardia alni sp. nov. The genomes of the N. alni and N. vaccinii strains contained 36 and 29 natural product-biosynthetic gene clusters, respectively, many of which were predicted to encode for a broad range of novel specialised products, notably antibiotics. Genome mining of the N. alni strain and the type strains of its closest phylogenetic neighbours revealed the presence of genes associated with direct and indirect mechanisms that promote plant growth. The core genomes of these strains mainly consisted of genes involved in amino acid transport and metabolism, energy production and conversion and transcription. Conclusions Our genome-based taxonomic study showed that isolate ncl2T formed a new centre of evolutionary variation within the genus Nocardia. This novel endophytic strain contained natural product biosynthetic gene clusters predicted to synthesize novel specialised products, notably antibiotics and genes associated with the expression of plant growth promoting compounds.

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“…Amycolatopsis sp. HCa4 isolated from the gut of locusts (Locusta migratoria) produces amycolamycin A which is selectively cytotoxic to the M231 breast cancer cell line [204] and rifamorpholines A-E [13]. Rifamorpholines represent the new subclass of rifamycin antibiotics with an unprecedented 5/6/6/6 fused tetracyclic ring system and an unusually modified polyketide chain.…”

Section: Antibiotics Produced By Amycolatopsis Isolated From Poorly Studied Ecological Habitatsmentioning

confidence: 99%

Looking Back to Amycolatopsis: History of the Antibiotic Discovery and Future Prospects

2021

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The emergence of antibiotic-resistant pathogenic bacteria in recent decades leads us to an urgent need for the development of new antibacterial agents. The species of the genus Amycolatopsis are known as producers of secondary metabolites that are used in medicine and agriculture. The complete genome sequences of the Amycolatopsis demonstrate a wide variety of biosynthetic gene clusters, which highlights the potential ability of actinomycetes of this genus to produce new antibiotics. In this review, we summarize information about antibiotics produced by Amycolatopsis species. This knowledge demonstrates the prospects for further study of this genus as an enormous source of antibiotics.

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Amycolamycins A and B, Two Enediyne-Derived Compounds from a Locust-Associated Actinomycete

Cited by 31 publications

References 26 publications

The secondary metabolites of rare actinomycetes: chemistry and bioactivity

The secondary metabolites of rare actinomycetes: chemistry and bioactivity

Genome insights into the pharmaceutical and plant growth promoting features of the novel species Nocardia alni sp. nov

Looking Back to Amycolatopsis: History of the Antibiotic Discovery and Future Prospects

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