AMV RNA Transcription in Cell-free Systems and Properties of In Vitro Chromatin-directed RNA Synthesis

Monroy, Gladys; Jacquet, Michel; Groner, Yoram; Hurwitz, Jerard

doi:10.1101/sqb.1974.039.01.119

Cited by 7 publications

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“…The viral DNA has been shown to be transcribed by cellular DNA-dependent RNA polymerase II by virtue of its sensitivity to the drug a-amanitin (31). No large precursor of M-MLV genomic RNA has been detected in the infected cells (32,33).…”

Section: Resultsmentioning

confidence: 99%

Structure of Moloney murine leukemia viral DNA: nucleotide sequence of the 5' long terminal repeat and adjacent cellular sequences.

Beveren¹,

Goddard²,

Berns³

et al. 1980

Proc. Natl. Acad. Sci. U.S.A.

124

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Some unintegrated and all integrated forms of murine leukemia viral DNA contain long terminal repeats (LT s). The entire nucleotide sequence of the LTR and adjacent cellular sequences at the 5' end of a cloned integrated proviral DNA obtained from BALB/Mo mouse has been determined. It was compared to the nucleotide sequence of the LTR at the 3' end. The results indicate: (i) a direct 517-nucleotide repeat at the 5' and 3' termini; (ii) 145 nucleotides out of 517 nucleotides represent sequences between the 5'-CAP nucleotide and 3' end of the primer tRNA (strong-stop DNA); (ifi) an li-nucleotide inverted repeat is present at the ends of the 5'-LTR and a total of 17 out of21 nucleotides at the termini are inverted repeats; (iv) sequences CAATAAAAG (at positions -24 to -31) and CAATAAAC (at positions +46 to +53) resembling the hypothetical DNA-dependent RNA polymerase II promoter site can be identified in the 5'-LTR; (v) the sequence GAAA appears to be repeated on both sides of the junction of viral and cellular sequences; and (vi) in analogy with the bacterial transposons, the presence of an inverted reat seuence at the termini of 5'-LTR suggests that M-MLV also has the integration properties of a transposon.

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Section: Resultsmentioning

confidence: 99%