Amputation de membre secondaire à une morsure de vipère

Kouassi, K.J.E.; M’bra, Kouamé Innocent; Sery, Bada Justin Léopold Niaore; Yao, Loukou Blaise; Krah, Koffi Léopold; Lohourou, Grah Franck; Kouassi, A.A.N.; Traoré, Ibrahim Alain; Asséré, Y.A.G.R.A.; Kodo, M

doi:10.1016/j.arcped.2016.12.013

Cited by 6 publications

(4 citation statements)

References 5 publications

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“…In addition, local damage is not well neutralized by conventional therapy and may result in permanent disability and amputation of the affected limb. This occurs mainly due to the rapid action of some toxins, making treatment even more challenging, especially when snakebites occur in hard-to-access regions of the country [4,14,38,39]. Another negative point of therapy with current antivenoms is that their production is a complex process of obtaining the antigens that involves the maintenance of snakes and extraction of the venom, the maintenance and immunization of large mammals, and obtaining and purifying the plasma to obtain the antigens.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Inhibitory Potential of Synthetic Peptides Homologous to CDR3 Regions of a Monoclonal Antibody against Bothropic Venom Serine Proteases

Saladini,

Magalhães-Junior,

da Silva

et al. 2024

IJMS

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Snakebite accidents, neglected tropical diseases per the WHO, pose a significant public health threat due to their severity and frequency. Envenomation by Bothrops genus snakes leads to severe manifestations due to proteolytic enzymes. While the antibothropic serum produced by the Butantan Institute saves lives, its efficacy is limited as it fails to neutralize certain serine proteases. Hence, developing new-generation antivenoms, like monoclonal antibodies, is crucial. This study aimed to explore the inhibitory potential of synthetic peptides homologous to the CDR3 regions of a monoclonal antibody targeting a snake venom thrombin-like enzyme (SVTLE) from B. atrox venom. Five synthetic peptides were studied, all stable against hydrolysis by venoms and serine proteases. Impressively, four peptides demonstrated uncompetitive SVTLE inhibition, with Ki values ranging from 10−6 to 10−7 M. These findings underscore the potential of short peptides homologous to CDR3 regions in blocking snake venom toxins, suggesting their promise as the basis for new-generation antivenoms. Thus, this study offers potential advancements in combatting snakebites, addressing a critical public health challenge in tropical and subtropical regions.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the Inhibitory Potential of Synthetic Peptides Homologous to CDR3 Regions of a Monoclonal Antibody against Bothropic Venom Serine Proteases

Saladini,

Magalhães-Junior,

da Silva

et al. 2024

IJMS

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“…Causa um número elevado de vítimas que podem evoluir para morte, principalmente nas áreas rurais, onde as comunidades são as que mais sofrem por viverem longe dos serviços de saúde que geralmente estão, inadequadamente, equipados para atender estas emergências médicas (Ainsworth et al, 2018). A amputação de membros constitui uma das consequências graves dos envenenamentos, sendo as complicações locais e sistémicas mais frequentes em crianças do que em adultos (Kouassi et al, 2017). Estas resultam em debilidades permanentes e morte (Tahis et al, 2015) que afetam cerca de 3% das vítimas (Guidlolin et al, 2010).…”

Section: Introductionunclassified

Envenenamento fatal por mordedura de Naja Nigricollis em Angola e uso da terapia tradicional: um caso clínico em Benguela

Oliveira

Kilembeketa²,

Bardaji³

et al. 2020

RevSALUS

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Introdução: O envenenamento por mordedura de serpentes constitui um importante problema de Saúde Pública e uma emergência médico-cirúrgica frequente, que afecta as comunidades rurais de África. É responsável por elevado número de vítimas que podem evoluir para morte, principalmente nas áreas rurais, onde estas comunidades são as que mais sofrem por viverem longe dos serviços de saúde, acabando por submeterem-se a tratamentos tradicionais impedindo o manuseio precoce e adequado dos casos em ambiente hospitalar. O presente artigo tem como objectivo descrever o primeiro caso clínico fatal ocorrido numa criança na Província de Benguela - Angola com envenenamento por naja nigricollis, a espécie que é considerada a segunda mais perigosa de África a seguir à Dendroaspis polylepsis. Trata-se de uma criança que foi mordida de noite no pescoço e pé direito. Durante cinco dias os pais submeteram-na a tratamento tradicional fitoterapêutico com um curandeiro, tendo a mesma acorrido ao Hospital Geral de Benguela com um quadro séptico de fasceíte necrotizante grave, anemia severa tendo-se realizado a necrectomia das regiões afectadas e tratamento adequado da anemia. Resultados: A morte da criança após 4 dias nos cuidados intensivos do mesmo Hospital. Conclusões: A fatalidade deste caso clínico demonstra que apesar do manuseio clinico cirúrgico tardio adequado o espectro da gravidade do envenenamento por naja nigricollis ainda constitui uma realidade que poderia ser evitada se educássemos as nossas comunidades rurais em relação à crença do uso de terapias tradicionais nos acidentes ofídicos em Angola.

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“…A significant fraction of the snake-bite survivors may have long-term disabilities and poor quality of life [11]. The hemorrhage is promoted by SVMPs which are the venom enzymes responsible for local and systemic disturbance of the hemostatic system [12,13].…”

Section: Introductionmentioning

confidence: 99%

Human Monoclonal scFvs that Neutralize Fribrinogenolytic Activity of Kaouthiagin, a Zinc-Metalloproteinase in Cobra (Naja kaouthia) Venom

Khanongnoi

Phanthong

Reamtong

et al. 2018

Toxins

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Snake venom-metalloproteinases (SVMPs) are the primary factors that disturb hemostasis and cause hemorrhage in the venomous snake bitten subjects. Kaouthiagin is a unique SVMP that binds and cleaves von Willebrand factor (vWF) at a specific peptide bond leading to inhibition of platelet aggregation, which enhances the hemorrhage. Kaouthiagin is a low abundant venom component of Thai cobra (Naja kaouthia); thus, most horse-derived antivenins used for cobra bite treatment do not contain adequate anti-kaouthiagin. This study aimed to produce human single-chain antibody variable fragments (HuscFvs) that bind to and interfere with kaouthiagin activity for further clinical use. Kaouthiagin was purified from N. kaouthia-holovenom by a single-step gel-filtration chromatography. The purified venom component was used in phage-biopanning to select the kaouthiagin-bound HuscFv-displayed-phage clones from a HuscFv-phage display library. The selected phages were used to infect Escherichia coli bacteria. Soluble HuscFvs expressed by three phage-transformed-E. coli clones interfered with cobra kaouthiagin binding to human vWF. Computerized simulation indicated that HuscFv of two phage-transformed E. coli clones formed contact interface with kaouthiagin residues at or near catalytic site and effectively inhibited fibrinogenolytic activity of the kaouthiagin. The HuscFvs have therapeutic potential as an adjunct of antivenins in treatment of bleeding caused by venomous snakebites.

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Amputation de membre secondaire à une morsure de vipère

Cited by 6 publications

References 5 publications

Evaluation of the Inhibitory Potential of Synthetic Peptides Homologous to CDR3 Regions of a Monoclonal Antibody against Bothropic Venom Serine Proteases

Evaluation of the Inhibitory Potential of Synthetic Peptides Homologous to CDR3 Regions of a Monoclonal Antibody against Bothropic Venom Serine Proteases

Envenenamento fatal por mordedura de Naja Nigricollis em Angola e uso da terapia tradicional: um caso clínico em Benguela

Human Monoclonal scFvs that Neutralize Fribrinogenolytic Activity of Kaouthiagin, a Zinc-Metalloproteinase in Cobra (Naja kaouthia) Venom

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